Early detection using dual-specific probe technique for genetic mutation of congenital cataract lens protein
AIM: To screen congenital cataract in the early stage using dual-specific probe technique composed of DNA hybridization and chromogenic probe. <p>METHODS: The mRNA was isolated from venous blood of 14 congenital cataract patients with autosomal dominant inheritance in 3 chromosomes. Based on g...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2018-06-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/cn_publish/2018/6/201806006.pdf |
| _version_ | 1818982667359617024 |
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| author | Yu-Juan Cao Jun Shao Ying-Qing Yu Yong Yao Jing Zhu Yu-Jing Tian Jia Cao |
| author_facet | Yu-Juan Cao Jun Shao Ying-Qing Yu Yong Yao Jing Zhu Yu-Jing Tian Jia Cao |
| author_sort | Yu-Juan Cao |
| collection | DOAJ |
| description | AIM: To screen congenital cataract in the early stage using dual-specific probe technique composed of DNA hybridization and chromogenic probe. <p>METHODS: The mRNA was isolated from venous blood of 14 congenital cataract patients with autosomal dominant inheritance in 3 chromosomes. Based on genetic mutats of CRYAB gene, DNA probe, capture probe and signal probe were designed. The sandwich structures containing capture probe, DNA probe and signal probe was used to detect genetic mutants in 8 samples from one family; and ELISA was used to detect the contents fluctuation of Crystallin alpha B. <p>RESULTS: The dual-specific probe technique detected the minimum genetic mutation of Crystallin alpha B in congenital cataract samples, various mutations detection rate was between 99.5% and 99.7%. In ELISA detection, serum Crystallin alpha B level increased, and detection rate was 85.9%. Compared with ELISA assay, this novel assay was more sensitive. <p>CONCLUSION: The novel dual-specific probe method is quite convenient for detection of genetic mutants of congenital cataract; and for its high sensitivity and repeatability, it is of great potential in clinically prenatal diagnosis, and it might play significant roles in eugenic and superior nurture. |
| first_indexed | 2024-12-20T17:50:51Z |
| format | Article |
| id | doaj.art-7a676152de734895906e05824c3e9de9 |
| institution | Directory Open Access Journal |
| issn | 1672-5123 1672-5123 |
| language | English |
| last_indexed | 2024-12-20T17:50:51Z |
| publishDate | 2018-06-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-7a676152de734895906e05824c3e9de92022-12-21T19:30:51ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232018-06-011861004100910.3980/j.issn.1672-5123.2018.6.06Early detection using dual-specific probe technique for genetic mutation of congenital cataract lens proteinYu-Juan Cao0Jun Shao1Ying-Qing Yu2Yong Yao3Jing Zhu4Yu-Jing Tian5Jia Cao6Department of Ophthalmology, Wuxi Children's Hospital, Wuxi 214023, Jiangsu Province, ChinaDepartment of Ophthalmology, Wuxi People's Hospital, Wuxi 214023, Jiangsu Province, ChinaDepartment of Ophthalmology, Wuxi Children's Hospital, Wuxi 214023, Jiangsu Province, ChinaDepartment of Ophthalmology, Wuxi People's Hospital, Wuxi 214023, Jiangsu Province, ChinaDepartment of Ophthalmology, Wuxi People's Hospital, Wuxi 214023, Jiangsu Province, ChinaDepartment of Ophthalmology, Wuxi Children's Hospital, Wuxi 214023, Jiangsu Province, ChinaDepartment of Ophthalmology, Wuxi Children's Hospital, Wuxi 214023, Jiangsu Province, ChinaAIM: To screen congenital cataract in the early stage using dual-specific probe technique composed of DNA hybridization and chromogenic probe. <p>METHODS: The mRNA was isolated from venous blood of 14 congenital cataract patients with autosomal dominant inheritance in 3 chromosomes. Based on genetic mutats of CRYAB gene, DNA probe, capture probe and signal probe were designed. The sandwich structures containing capture probe, DNA probe and signal probe was used to detect genetic mutants in 8 samples from one family; and ELISA was used to detect the contents fluctuation of Crystallin alpha B. <p>RESULTS: The dual-specific probe technique detected the minimum genetic mutation of Crystallin alpha B in congenital cataract samples, various mutations detection rate was between 99.5% and 99.7%. In ELISA detection, serum Crystallin alpha B level increased, and detection rate was 85.9%. Compared with ELISA assay, this novel assay was more sensitive. <p>CONCLUSION: The novel dual-specific probe method is quite convenient for detection of genetic mutants of congenital cataract; and for its high sensitivity and repeatability, it is of great potential in clinically prenatal diagnosis, and it might play significant roles in eugenic and superior nurture.http://ies.ijo.cn/cn_publish/2018/6/201806006.pdfdual-specific probe techniquecongenital cataractlens protein genelens protein gene |
| spellingShingle | Yu-Juan Cao Jun Shao Ying-Qing Yu Yong Yao Jing Zhu Yu-Jing Tian Jia Cao Early detection using dual-specific probe technique for genetic mutation of congenital cataract lens protein Guoji Yanke Zazhi dual-specific probe technique congenital cataract lens protein gene lens protein gene |
| title | Early detection using dual-specific probe technique for genetic mutation of congenital cataract lens protein |
| title_full | Early detection using dual-specific probe technique for genetic mutation of congenital cataract lens protein |
| title_fullStr | Early detection using dual-specific probe technique for genetic mutation of congenital cataract lens protein |
| title_full_unstemmed | Early detection using dual-specific probe technique for genetic mutation of congenital cataract lens protein |
| title_short | Early detection using dual-specific probe technique for genetic mutation of congenital cataract lens protein |
| title_sort | early detection using dual specific probe technique for genetic mutation of congenital cataract lens protein |
| topic | dual-specific probe technique congenital cataract lens protein gene lens protein gene |
| url | http://ies.ijo.cn/cn_publish/2018/6/201806006.pdf |
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