Continuous bioreactor production of polyhydroxyalkanoates in Haloferax mediterranei
In this work, the viability of continuous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) production with controlled composition in Haloferax mediterranei when fed volatile fatty acids is demonstrated. Continuous fermentations showed to greatly outperform batch fermentations with continuous feed...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1220271/full |
| _version_ | 1797687126061481984 |
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| author | Mariana Parroquin-Gonzalez James Winterburn |
| author_facet | Mariana Parroquin-Gonzalez James Winterburn |
| author_sort | Mariana Parroquin-Gonzalez |
| collection | DOAJ |
| description | In this work, the viability of continuous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) production with controlled composition in Haloferax mediterranei when fed volatile fatty acids is demonstrated. Continuous fermentations showed to greatly outperform batch fermentations with continuous feeding. Operating the bioreactor continuously allowed for PHBV productivity normalised by cell density to increase from 0.29 to 0.38 mg L−1 h−1, in previous continuously fed-fed batch fermentations, to 0.87 and 1.43 mg L−1 h−1 in a continuous mode of operation for 0.1 and 0.25 M carbon concentrations in the media respectively. Continuous bioreactor experiments were carried out for 100 h, maintaining control over the copolymer composition at around 30 mol% 3-hydroxyvalerate 3HV. This work presents the first continuous production of PHBV in Haloferax mediterranei which continuously delivers polymer at a higher productivity, compared to fed-batch modes of operation. Operating bioreactors continuously whilst maintaining control over copolymer composition brings new processing opportunities for increasing biopolymer production capacity, a crucial step towards the wider industrialisation of polyhydroxyalkanoates (PHAs). |
| first_indexed | 2024-03-12T01:13:47Z |
| format | Article |
| id | doaj.art-7a682f1d80c840f3bb7a33f4453e3c4a |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-12T01:13:47Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-7a682f1d80c840f3bb7a33f4453e3c4a2023-09-13T21:51:31ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-09-011110.3389/fbioe.2023.12202711220271Continuous bioreactor production of polyhydroxyalkanoates in Haloferax mediterraneiMariana Parroquin-GonzalezJames WinterburnIn this work, the viability of continuous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) production with controlled composition in Haloferax mediterranei when fed volatile fatty acids is demonstrated. Continuous fermentations showed to greatly outperform batch fermentations with continuous feeding. Operating the bioreactor continuously allowed for PHBV productivity normalised by cell density to increase from 0.29 to 0.38 mg L−1 h−1, in previous continuously fed-fed batch fermentations, to 0.87 and 1.43 mg L−1 h−1 in a continuous mode of operation for 0.1 and 0.25 M carbon concentrations in the media respectively. Continuous bioreactor experiments were carried out for 100 h, maintaining control over the copolymer composition at around 30 mol% 3-hydroxyvalerate 3HV. This work presents the first continuous production of PHBV in Haloferax mediterranei which continuously delivers polymer at a higher productivity, compared to fed-batch modes of operation. Operating bioreactors continuously whilst maintaining control over copolymer composition brings new processing opportunities for increasing biopolymer production capacity, a crucial step towards the wider industrialisation of polyhydroxyalkanoates (PHAs).https://www.frontiersin.org/articles/10.3389/fbioe.2023.1220271/fullbioreactorfermentationcontinuousHaloferax mediterraneipolyhydroxyalkanoatesPoly(3-hydroxybutryrate-co-3-hydroxyvalerate) |
| spellingShingle | Mariana Parroquin-Gonzalez James Winterburn Continuous bioreactor production of polyhydroxyalkanoates in Haloferax mediterranei Frontiers in Bioengineering and Biotechnology bioreactor fermentation continuous Haloferax mediterranei polyhydroxyalkanoates Poly(3-hydroxybutryrate-co-3-hydroxyvalerate) |
| title | Continuous bioreactor production of polyhydroxyalkanoates in Haloferax mediterranei |
| title_full | Continuous bioreactor production of polyhydroxyalkanoates in Haloferax mediterranei |
| title_fullStr | Continuous bioreactor production of polyhydroxyalkanoates in Haloferax mediterranei |
| title_full_unstemmed | Continuous bioreactor production of polyhydroxyalkanoates in Haloferax mediterranei |
| title_short | Continuous bioreactor production of polyhydroxyalkanoates in Haloferax mediterranei |
| title_sort | continuous bioreactor production of polyhydroxyalkanoates in haloferax mediterranei |
| topic | bioreactor fermentation continuous Haloferax mediterranei polyhydroxyalkanoates Poly(3-hydroxybutryrate-co-3-hydroxyvalerate) |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1220271/full |
| work_keys_str_mv | AT marianaparroquingonzalez continuousbioreactorproductionofpolyhydroxyalkanoatesinhaloferaxmediterranei AT jameswinterburn continuousbioreactorproductionofpolyhydroxyalkanoatesinhaloferaxmediterranei |