Transcriptional Regulation of the Outer Membrane Protein A in <i>Acinetobacter baumannii</i>
<i>Acinetobacter baumannii</i> is known for its virulence in severely ill, hospitalized patients and for exhibiting multidrug resistance. <i>A. baumannii</i> infection treatment poses a serious problem in clinical environments. The outer membrane protein A (<i>OmpA</...
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MDPI AG
2020-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/5/706 |
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| author | Kyu-Wan Oh Kyeongmin Kim Md. Maidul Islam Hye-Won Jung Daejin Lim Je Chul Lee Minsang Shin |
| author_facet | Kyu-Wan Oh Kyeongmin Kim Md. Maidul Islam Hye-Won Jung Daejin Lim Je Chul Lee Minsang Shin |
| author_sort | Kyu-Wan Oh |
| collection | DOAJ |
| description | <i>Acinetobacter baumannii</i> is known for its virulence in severely ill, hospitalized patients and for exhibiting multidrug resistance. <i>A. baumannii</i> infection treatment poses a serious problem in clinical environments. The outer membrane protein A (<i>OmpA</i>) of the Acinetobacter genus is involved in bacterial virulence. Regulatory factors of <i>OmpA</i> in the post-transcriptional stage have been previously identified. However, the regulatory factors that act before the transcriptional stage remain unclear. We investigated the <i>A1S_0316</i> gene that encodes a putative transcription factor for <i>OmpA</i> expression in <i>A. baumannii</i>. A1S_0316 was purified and examined using size-exclusion chromatography, which revealed that it forms an oligomer. The binding affinity of A1S_0316 to the <i>OmpA</i> promoter region was also examined. We compared the binding affinity to the <i>OmpA</i> promotor region between A1S_0316 and the AbH-NS protein. A1S_0316 showed higher binding affinity to the <i>OmpA</i> promotor region than did H-NS. We examined the regulatory effect of these proteins on <i>OmpA</i> expression in <i>A. baumannii</i> using real-time qPCR and various in vitro tools. Our results indicated that A1S_0316 acts as an anti-repressor on the promotor region of the <i>OmpA</i> gene by inhibiting the binding of the AbH-NS protein. This study was the first demonstration of the transcriptional regulation of <i>OmpA</i> expression. |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T19:54:33Z |
| publishDate | 2020-05-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-7a6ac55e6c1f49c795f9556584d4f1192023-11-20T00:04:46ZengMDPI AGMicroorganisms2076-26072020-05-018570610.3390/microorganisms8050706Transcriptional Regulation of the Outer Membrane Protein A in <i>Acinetobacter baumannii</i>Kyu-Wan Oh0Kyeongmin Kim1Md. Maidul Islam2Hye-Won Jung3Daejin Lim4Je Chul Lee5Minsang Shin6Department of Microbiology, School of Medicine, Kyungpook National University, 680 Gukchaebosang-ro, Jung-gu, Daegu 41944, KoreaDepartment of Microbiology, School of Medicine, Kyungpook National University, 680 Gukchaebosang-ro, Jung-gu, Daegu 41944, KoreaDepartment of Microbiology, School of Medicine, Kyungpook National University, 680 Gukchaebosang-ro, Jung-gu, Daegu 41944, KoreaDepartment of Microbiology, School of Medicine, Kyungpook National University, 680 Gukchaebosang-ro, Jung-gu, Daegu 41944, KoreaDepartment of Microbiology, School of Medicine, Chonnam National University, Gwangju 61468, KoreaDepartment of Microbiology, School of Medicine, Kyungpook National University, 680 Gukchaebosang-ro, Jung-gu, Daegu 41944, KoreaDepartment of Microbiology, School of Medicine, Kyungpook National University, 680 Gukchaebosang-ro, Jung-gu, Daegu 41944, Korea<i>Acinetobacter baumannii</i> is known for its virulence in severely ill, hospitalized patients and for exhibiting multidrug resistance. <i>A. baumannii</i> infection treatment poses a serious problem in clinical environments. The outer membrane protein A (<i>OmpA</i>) of the Acinetobacter genus is involved in bacterial virulence. Regulatory factors of <i>OmpA</i> in the post-transcriptional stage have been previously identified. However, the regulatory factors that act before the transcriptional stage remain unclear. We investigated the <i>A1S_0316</i> gene that encodes a putative transcription factor for <i>OmpA</i> expression in <i>A. baumannii</i>. A1S_0316 was purified and examined using size-exclusion chromatography, which revealed that it forms an oligomer. The binding affinity of A1S_0316 to the <i>OmpA</i> promoter region was also examined. We compared the binding affinity to the <i>OmpA</i> promotor region between A1S_0316 and the AbH-NS protein. A1S_0316 showed higher binding affinity to the <i>OmpA</i> promotor region than did H-NS. We examined the regulatory effect of these proteins on <i>OmpA</i> expression in <i>A. baumannii</i> using real-time qPCR and various in vitro tools. Our results indicated that A1S_0316 acts as an anti-repressor on the promotor region of the <i>OmpA</i> gene by inhibiting the binding of the AbH-NS protein. This study was the first demonstration of the transcriptional regulation of <i>OmpA</i> expression.https://www.mdpi.com/2076-2607/8/5/706<i>Acinetobacter baumannii</i><i>OmpA</i><i>A1S_0316</i><i>H-NS</i>transcription regulator |
| spellingShingle | Kyu-Wan Oh Kyeongmin Kim Md. Maidul Islam Hye-Won Jung Daejin Lim Je Chul Lee Minsang Shin Transcriptional Regulation of the Outer Membrane Protein A in <i>Acinetobacter baumannii</i> Microorganisms <i>Acinetobacter baumannii</i> <i>OmpA</i> <i>A1S_0316</i> <i>H-NS</i> transcription regulator |
| title | Transcriptional Regulation of the Outer Membrane Protein A in <i>Acinetobacter baumannii</i> |
| title_full | Transcriptional Regulation of the Outer Membrane Protein A in <i>Acinetobacter baumannii</i> |
| title_fullStr | Transcriptional Regulation of the Outer Membrane Protein A in <i>Acinetobacter baumannii</i> |
| title_full_unstemmed | Transcriptional Regulation of the Outer Membrane Protein A in <i>Acinetobacter baumannii</i> |
| title_short | Transcriptional Regulation of the Outer Membrane Protein A in <i>Acinetobacter baumannii</i> |
| title_sort | transcriptional regulation of the outer membrane protein a in i acinetobacter baumannii i |
| topic | <i>Acinetobacter baumannii</i> <i>OmpA</i> <i>A1S_0316</i> <i>H-NS</i> transcription regulator |
| url | https://www.mdpi.com/2076-2607/8/5/706 |
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