Expression of NEDD4L and ENaC in Urinary Extracellular Vesicles in Pre-eclampsia
Objective To assess changes in expression of renal epithelial sodium channel (ENaC) and NEDD4L, a ubiquitin ligase, in urinary extracellular vesicles (UEV) of pre-eclamptic women compared to normal pregnant controls. Methods Urine was collected from pre-eclamptic women (PE, n = 20) or during normal...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
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| Series: | Hypertension in Pregnancy |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/10641955.2023.2232029 |
| Summary: | Objective To assess changes in expression of renal epithelial sodium channel (ENaC) and NEDD4L, a ubiquitin ligase, in urinary extracellular vesicles (UEV) of pre-eclamptic women compared to normal pregnant controls. Methods Urine was collected from pre-eclamptic women (PE, n = 20) or during normal pregnancy (NP, n = 20). UEV were separated by differential ultracentrifugation. NEDD4L, α-ENaC and γ-ENaC were identified by immunoblotting. Results There was no difference in the expression of NEDD4L (p = 0.17) and α-ENaC (p = 0.10). PE subjects showed increased expression of γ-ENaC by 6.9-fold compared to NP (p < 0.0001). Conclusion ENaC expression is upregulated in UEV of pre-eclamptic subjects but was not associated with changes in NEDD4L. |
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| ISSN: | 1064-1955 1525-6065 |