Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures
A phenotypic screening of 12 industrial yeast strains and the well-studied laboratory strain CEN.PK113-7D at cultivation temperatures between 12 °C and 40 °C revealed significant differences in maximum growth rates and temperature tolerance. From those 12, two strains, one performing best at 12 °C a...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-06-01
|
| Series: | Biotechnology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X20300175 |
| _version_ | 1819157989430394880 |
|---|---|
| author | Ka Ying Florence Lip Estéfani García-Ríos Carlos E. Costa José Manuel Guillamón Lucília Domingues José Teixeira Walter M. van Gulik |
| author_facet | Ka Ying Florence Lip Estéfani García-Ríos Carlos E. Costa José Manuel Guillamón Lucília Domingues José Teixeira Walter M. van Gulik |
| author_sort | Ka Ying Florence Lip |
| collection | DOAJ |
| description | A phenotypic screening of 12 industrial yeast strains and the well-studied laboratory strain CEN.PK113-7D at cultivation temperatures between 12 °C and 40 °C revealed significant differences in maximum growth rates and temperature tolerance. From those 12, two strains, one performing best at 12 °C and the other at 40 °C, plus the laboratory strain, were selected for further physiological characterization in well-controlled bioreactors. The strains were grown in anaerobic chemostats, at a fixed specific growth rate of 0.03 h−1 and sequential batch cultures at 12 °C, 30 °C, and 39 °C. We observed significant differences in biomass and ethanol yields on glucose, biomass protein and storage carbohydrate contents, and biomass yields on ATP between strains and cultivation temperatures. Increased temperature tolerance coincided with higher energetic efficiency of cell growth, indicating that temperature intolerance is a result of energy wasting processes, such as increased turnover of cellular components (e.g. proteins) due to temperature induced damage. |
| first_indexed | 2024-12-22T16:17:31Z |
| format | Article |
| id | doaj.art-7a7100306cea43f59f89b6880418798e |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-22T16:17:31Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-7a7100306cea43f59f89b6880418798e2022-12-21T18:20:21ZengElsevierBiotechnology Reports2215-017X2020-06-0126e00462Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperaturesKa Ying Florence Lip0Estéfani García-Ríos1Carlos E. Costa2José Manuel Guillamón3Lucília Domingues4José Teixeira5Walter M. van Gulik6Department of Biotechnology, Delft University of Technology, Delft 2629HZ, the Netherlands; Corresponding authors.Food Biotechnology Department, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Valencia, SpainCentre of Biological Engineering, University of Minho, Braga 4710-057, PortugalFood Biotechnology Department, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Valencia, SpainCentre of Biological Engineering, University of Minho, Braga 4710-057, PortugalCentre of Biological Engineering, University of Minho, Braga 4710-057, PortugalDepartment of Biotechnology, Delft University of Technology, Delft 2629HZ, the Netherlands; Corresponding authors.A phenotypic screening of 12 industrial yeast strains and the well-studied laboratory strain CEN.PK113-7D at cultivation temperatures between 12 °C and 40 °C revealed significant differences in maximum growth rates and temperature tolerance. From those 12, two strains, one performing best at 12 °C and the other at 40 °C, plus the laboratory strain, were selected for further physiological characterization in well-controlled bioreactors. The strains were grown in anaerobic chemostats, at a fixed specific growth rate of 0.03 h−1 and sequential batch cultures at 12 °C, 30 °C, and 39 °C. We observed significant differences in biomass and ethanol yields on glucose, biomass protein and storage carbohydrate contents, and biomass yields on ATP between strains and cultivation temperatures. Increased temperature tolerance coincided with higher energetic efficiency of cell growth, indicating that temperature intolerance is a result of energy wasting processes, such as increased turnover of cellular components (e.g. proteins) due to temperature induced damage.http://www.sciencedirect.com/science/article/pii/S2215017X20300175ChemostatEnergetic efficiencyTemperature toleranceSaccharomycesSBR |
| spellingShingle | Ka Ying Florence Lip Estéfani García-Ríos Carlos E. Costa José Manuel Guillamón Lucília Domingues José Teixeira Walter M. van Gulik Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures Biotechnology Reports Chemostat Energetic efficiency Temperature tolerance Saccharomyces SBR |
| title | Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures |
| title_full | Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures |
| title_fullStr | Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures |
| title_full_unstemmed | Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures |
| title_short | Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures |
| title_sort | selection and subsequent physiological characterization of industrial saccharomyces cerevisiae strains during continuous growth at sub and supra optimal temperatures |
| topic | Chemostat Energetic efficiency Temperature tolerance Saccharomyces SBR |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X20300175 |
| work_keys_str_mv | AT kayingflorencelip selectionandsubsequentphysiologicalcharacterizationofindustrialsaccharomycescerevisiaestrainsduringcontinuousgrowthatsubandsupraoptimaltemperatures AT estefanigarciarios selectionandsubsequentphysiologicalcharacterizationofindustrialsaccharomycescerevisiaestrainsduringcontinuousgrowthatsubandsupraoptimaltemperatures AT carlosecosta selectionandsubsequentphysiologicalcharacterizationofindustrialsaccharomycescerevisiaestrainsduringcontinuousgrowthatsubandsupraoptimaltemperatures AT josemanuelguillamon selectionandsubsequentphysiologicalcharacterizationofindustrialsaccharomycescerevisiaestrainsduringcontinuousgrowthatsubandsupraoptimaltemperatures AT luciliadomingues selectionandsubsequentphysiologicalcharacterizationofindustrialsaccharomycescerevisiaestrainsduringcontinuousgrowthatsubandsupraoptimaltemperatures AT joseteixeira selectionandsubsequentphysiologicalcharacterizationofindustrialsaccharomycescerevisiaestrainsduringcontinuousgrowthatsubandsupraoptimaltemperatures AT waltermvangulik selectionandsubsequentphysiologicalcharacterizationofindustrialsaccharomycescerevisiaestrainsduringcontinuousgrowthatsubandsupraoptimaltemperatures |