Molecular subdivision of the marine diatom <it>Thalassiosira rotula</it> in relation to geographic distribution, genome size, and physiology
<p>Abstract</p> <p>Background</p> <p>Marine phytoplankton drift passively with currents, have high dispersal potentials and can be comprised of morphologically cryptic species. To examine molecular subdivision in the marine diatom <it>Thalassiosira rotula,</it&...
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| Format: | Article |
| Language: | English |
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BMC
2012-10-01
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| Series: | BMC Evolutionary Biology |
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| Online Access: | http://www.biomedcentral.com/1471-2148/12/209 |
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| author | Whittaker Kerry A Rignanese Dayna R Olson Robert J Rynearson Tatiana A |
| author_facet | Whittaker Kerry A Rignanese Dayna R Olson Robert J Rynearson Tatiana A |
| author_sort | Whittaker Kerry A |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Marine phytoplankton drift passively with currents, have high dispersal potentials and can be comprised of morphologically cryptic species. To examine molecular subdivision in the marine diatom <it>Thalassiosira rotula,</it> variations in rDNA sequence, genome size, and growth rate were examined among isolates collected from the Atlantic and Pacific Ocean basins. Analyses of rDNA included <it>T. gravida</it> because morphological studies have argued that <it>T. rotula</it> and <it>T. gravida</it> are conspecific.</p> <p>Results</p> <p>Culture collection isolates of <it>T. gravida</it> and <it>T. rotula</it> diverged by 7.0 ± 0.3% at the ITS1 and by 0.8 ± 0.03% at the 28S. Within <it>T. rotula</it>, field and culture collection isolates were subdivided into three lineages that diverged by 0.6 ± 0.3% at the ITS1 and 0% at the 28S. The predicted ITS1 secondary structure revealed no compensatory base pair changes among lineages. Differences in genome size were observed among isolates, but were not correlated with ITS1 lineages. Maximum acclimated growth rates of isolates revealed genotype by environment effects, but these were also not correlated with ITS1 lineages. In contrast, intra-individual variation in the multi-copy ITS1 revealed no evidence of recombination amongst lineages, and molecular clock estimates indicated that lineages diverged 0.68 Mya. The three lineages exhibited different geographic distributions and, with one exception, each field sample was dominated by a single lineage.</p> <p>Conclusions</p> <p>The degree of inter- and intra-specific divergence between <it>T. gravida</it> and <it>T. rotula</it> suggests they should continue to be treated as separate species. The phylogenetic distinction of the three closely-related <it>T. rotula</it> lineages was unclear. On the one hand, the lineages showed no physiological differences, no consistent genome size differences and no significant changes in the ITS1 secondary structure, suggesting there are no barriers to interbreeding among lineages. In contrast, analysis of intra-individual variation in the multicopy ITS1 as well as molecular clock estimates of divergence suggest these lineages have not interbred for significant periods of time. Given the current data, these lineages should be considered a single species. Furthermore, these <it>T. rotula</it> lineages may be ecologically relevant, given their differential abundance over large spatial scales.</p> |
| first_indexed | 2024-12-19T16:46:10Z |
| format | Article |
| id | doaj.art-7a74966137734b7fa9c39e35a4535391 |
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| issn | 1471-2148 |
| language | English |
| last_indexed | 2024-12-19T16:46:10Z |
| publishDate | 2012-10-01 |
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| spelling | doaj.art-7a74966137734b7fa9c39e35a45353912022-12-21T20:13:39ZengBMCBMC Evolutionary Biology1471-21482012-10-0112120910.1186/1471-2148-12-209Molecular subdivision of the marine diatom <it>Thalassiosira rotula</it> in relation to geographic distribution, genome size, and physiologyWhittaker Kerry ARignanese Dayna ROlson Robert JRynearson Tatiana A<p>Abstract</p> <p>Background</p> <p>Marine phytoplankton drift passively with currents, have high dispersal potentials and can be comprised of morphologically cryptic species. To examine molecular subdivision in the marine diatom <it>Thalassiosira rotula,</it> variations in rDNA sequence, genome size, and growth rate were examined among isolates collected from the Atlantic and Pacific Ocean basins. Analyses of rDNA included <it>T. gravida</it> because morphological studies have argued that <it>T. rotula</it> and <it>T. gravida</it> are conspecific.</p> <p>Results</p> <p>Culture collection isolates of <it>T. gravida</it> and <it>T. rotula</it> diverged by 7.0 ± 0.3% at the ITS1 and by 0.8 ± 0.03% at the 28S. Within <it>T. rotula</it>, field and culture collection isolates were subdivided into three lineages that diverged by 0.6 ± 0.3% at the ITS1 and 0% at the 28S. The predicted ITS1 secondary structure revealed no compensatory base pair changes among lineages. Differences in genome size were observed among isolates, but were not correlated with ITS1 lineages. Maximum acclimated growth rates of isolates revealed genotype by environment effects, but these were also not correlated with ITS1 lineages. In contrast, intra-individual variation in the multi-copy ITS1 revealed no evidence of recombination amongst lineages, and molecular clock estimates indicated that lineages diverged 0.68 Mya. The three lineages exhibited different geographic distributions and, with one exception, each field sample was dominated by a single lineage.</p> <p>Conclusions</p> <p>The degree of inter- and intra-specific divergence between <it>T. gravida</it> and <it>T. rotula</it> suggests they should continue to be treated as separate species. The phylogenetic distinction of the three closely-related <it>T. rotula</it> lineages was unclear. On the one hand, the lineages showed no physiological differences, no consistent genome size differences and no significant changes in the ITS1 secondary structure, suggesting there are no barriers to interbreeding among lineages. In contrast, analysis of intra-individual variation in the multicopy ITS1 as well as molecular clock estimates of divergence suggest these lineages have not interbred for significant periods of time. Given the current data, these lineages should be considered a single species. Furthermore, these <it>T. rotula</it> lineages may be ecologically relevant, given their differential abundance over large spatial scales.</p>http://www.biomedcentral.com/1471-2148/12/209PhytoplanktonPhylogeographyDispersalPhysiologyIntraspecific diversity |
| spellingShingle | Whittaker Kerry A Rignanese Dayna R Olson Robert J Rynearson Tatiana A Molecular subdivision of the marine diatom <it>Thalassiosira rotula</it> in relation to geographic distribution, genome size, and physiology BMC Evolutionary Biology Phytoplankton Phylogeography Dispersal Physiology Intraspecific diversity |
| title | Molecular subdivision of the marine diatom <it>Thalassiosira rotula</it> in relation to geographic distribution, genome size, and physiology |
| title_full | Molecular subdivision of the marine diatom <it>Thalassiosira rotula</it> in relation to geographic distribution, genome size, and physiology |
| title_fullStr | Molecular subdivision of the marine diatom <it>Thalassiosira rotula</it> in relation to geographic distribution, genome size, and physiology |
| title_full_unstemmed | Molecular subdivision of the marine diatom <it>Thalassiosira rotula</it> in relation to geographic distribution, genome size, and physiology |
| title_short | Molecular subdivision of the marine diatom <it>Thalassiosira rotula</it> in relation to geographic distribution, genome size, and physiology |
| title_sort | molecular subdivision of the marine diatom it thalassiosira rotula it in relation to geographic distribution genome size and physiology |
| topic | Phytoplankton Phylogeography Dispersal Physiology Intraspecific diversity |
| url | http://www.biomedcentral.com/1471-2148/12/209 |
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