SGK1 is essential for meiotic resumption in mammalian oocytes
In mammalian females, oocytes are stored in the ovary and meiosis is arrested at the diplotene stage of prophase I. When females reach puberty oocytes are selectively recruited in cycles to grow, overcome the meiotic arrest, complete the first meiotic division and become mature (ready for fertilizat...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | European Journal of Cell Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0171933522000139 |
| _version_ | 1818572084510457856 |
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| author | Edgar del Llano Rajan Iyyappan Daria Aleshkina Tomas Masek Michal Dvoran Zongliang Jiang Martin Pospisek Michal Kubelka Andrej Susor |
| author_facet | Edgar del Llano Rajan Iyyappan Daria Aleshkina Tomas Masek Michal Dvoran Zongliang Jiang Martin Pospisek Michal Kubelka Andrej Susor |
| author_sort | Edgar del Llano |
| collection | DOAJ |
| description | In mammalian females, oocytes are stored in the ovary and meiosis is arrested at the diplotene stage of prophase I. When females reach puberty oocytes are selectively recruited in cycles to grow, overcome the meiotic arrest, complete the first meiotic division and become mature (ready for fertilization). At a molecular level, the master regulator of prophase I arrest and meiotic resumption is the maturation-promoting factor (MPF) complex, formed by the active form of cyclin dependent kinase 1 (CDK1) and Cyclin B1. However, we still do not have complete information regarding the factors implicated in MPF activation.In this study we document that out of three mammalian serum-glucocorticoid kinase proteins (SGK1, SGK2, SGK3), mouse oocytes express only SGK1 with a phosphorylated (active) form dominantly localized in the nucleoplasm. Further, suppression of SGK1 activity in oocytes results in decreased CDK1 activation via the phosphatase cell division cycle 25B (CDC25B), consequently delaying or inhibiting nuclear envelope breakdown. Expression of exogenous constitutively active CDK1 can rescue the phenotype induced by SGK1 inhibition. These findings bring new insights into the molecular pathways acting upstream of MPF and a better understanding of meiotic resumption control by presenting a new key player SGK1 in mammalian oocytes. |
| first_indexed | 2024-12-14T18:52:41Z |
| format | Article |
| id | doaj.art-7a74b538a21346588f6ae9f0b1c05714 |
| institution | Directory Open Access Journal |
| issn | 0171-9335 |
| language | English |
| last_indexed | 2024-12-14T18:52:41Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | European Journal of Cell Biology |
| spelling | doaj.art-7a74b538a21346588f6ae9f0b1c057142022-12-21T22:51:11ZengElsevierEuropean Journal of Cell Biology0171-93352022-04-011012151210SGK1 is essential for meiotic resumption in mammalian oocytesEdgar del Llano0Rajan Iyyappan1Daria Aleshkina2Tomas Masek3Michal Dvoran4Zongliang Jiang5Martin Pospisek6Michal Kubelka7Andrej Susor8Laboratory of Biochemistry and Molecular Biology of Germ Cells, Institute of Animal Physiology and Genetics, CAS, Libechov, Czech Republic; Corresponding authors.Laboratory of Biochemistry and Molecular Biology of Germ Cells, Institute of Animal Physiology and Genetics, CAS, Libechov, Czech RepublicLaboratory of Biochemistry and Molecular Biology of Germ Cells, Institute of Animal Physiology and Genetics, CAS, Libechov, Czech RepublicLaboratory of RNA Biochemistry, Department of Genetics and Microbiology, Faculty of Science, Charles University, Viničná 5, Prague 128 44, Czech RepublicLaboratory of Biochemistry and Molecular Biology of Germ Cells, Institute of Animal Physiology and Genetics, CAS, Libechov, Czech RepublicSchool of Animal Sciences, AgCenter, Louisiana State University, Baton Rouge, LA 70803, United StatesLaboratory of RNA Biochemistry, Department of Genetics and Microbiology, Faculty of Science, Charles University, Viničná 5, Prague 128 44, Czech RepublicLaboratory of Biochemistry and Molecular Biology of Germ Cells, Institute of Animal Physiology and Genetics, CAS, Libechov, Czech RepublicLaboratory of Biochemistry and Molecular Biology of Germ Cells, Institute of Animal Physiology and Genetics, CAS, Libechov, Czech Republic; Corresponding authors.In mammalian females, oocytes are stored in the ovary and meiosis is arrested at the diplotene stage of prophase I. When females reach puberty oocytes are selectively recruited in cycles to grow, overcome the meiotic arrest, complete the first meiotic division and become mature (ready for fertilization). At a molecular level, the master regulator of prophase I arrest and meiotic resumption is the maturation-promoting factor (MPF) complex, formed by the active form of cyclin dependent kinase 1 (CDK1) and Cyclin B1. However, we still do not have complete information regarding the factors implicated in MPF activation.In this study we document that out of three mammalian serum-glucocorticoid kinase proteins (SGK1, SGK2, SGK3), mouse oocytes express only SGK1 with a phosphorylated (active) form dominantly localized in the nucleoplasm. Further, suppression of SGK1 activity in oocytes results in decreased CDK1 activation via the phosphatase cell division cycle 25B (CDC25B), consequently delaying or inhibiting nuclear envelope breakdown. Expression of exogenous constitutively active CDK1 can rescue the phenotype induced by SGK1 inhibition. These findings bring new insights into the molecular pathways acting upstream of MPF and a better understanding of meiotic resumption control by presenting a new key player SGK1 in mammalian oocytes.http://www.sciencedirect.com/science/article/pii/S0171933522000139OocyteMPFCDK1SGK1MeiosisNuclear envelope breakdown |
| spellingShingle | Edgar del Llano Rajan Iyyappan Daria Aleshkina Tomas Masek Michal Dvoran Zongliang Jiang Martin Pospisek Michal Kubelka Andrej Susor SGK1 is essential for meiotic resumption in mammalian oocytes European Journal of Cell Biology Oocyte MPF CDK1 SGK1 Meiosis Nuclear envelope breakdown |
| title | SGK1 is essential for meiotic resumption in mammalian oocytes |
| title_full | SGK1 is essential for meiotic resumption in mammalian oocytes |
| title_fullStr | SGK1 is essential for meiotic resumption in mammalian oocytes |
| title_full_unstemmed | SGK1 is essential for meiotic resumption in mammalian oocytes |
| title_short | SGK1 is essential for meiotic resumption in mammalian oocytes |
| title_sort | sgk1 is essential for meiotic resumption in mammalian oocytes |
| topic | Oocyte MPF CDK1 SGK1 Meiosis Nuclear envelope breakdown |
| url | http://www.sciencedirect.com/science/article/pii/S0171933522000139 |
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