In vitro isolation and expansion of neural stem cells NSCs
Neural stem cells (NSCs) are progenitor cells which have the ability to self‑renewal and potential for differentiating into neurons, oligodendrocytes, and astrocytes. The in vitro isolation, culturing, identification, cryopreservation were investigated to produce neural stem cells in culture as...
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| Format: | Article |
| Language: | Arabic |
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College of Science for Women, University of Baghdad
2023-06-01
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| Series: | Baghdad Science Journal |
| Subjects: | |
| Online Access: | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7280 |
| _version_ | 1797813943005085696 |
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| author | Maeda H. Mohammad Aous Kahtan Almzaien Ahmad A. Al-Joubory Ahmed M. Al-Shammari Ayser A. Ahmed Hiba K. Shaker Aseel K. Abedalsattar |
| author_facet | Maeda H. Mohammad Aous Kahtan Almzaien Ahmad A. Al-Joubory Ahmed M. Al-Shammari Ayser A. Ahmed Hiba K. Shaker Aseel K. Abedalsattar |
| author_sort | Maeda H. Mohammad |
| collection | DOAJ |
| description |
Neural stem cells (NSCs) are progenitor cells which have the ability to self‑renewal and potential for differentiating into neurons, oligodendrocytes, and astrocytes. The in vitro isolation, culturing, identification, cryopreservation were investigated to produce neural stem cells in culture as successful sources for further studies before using it for clinical trials. In this study, mouse bone marrow was the source of neural stem cells. The results of morphological study and immunocytochemistry of isolated cells showed that NSCs can be produced successfully and maintaining their self‑renewal and successfully forming neurosphere for multiple passages. The spheres preserved their morphology in culture and cryopreserved to be a ready source for use in experiments as a model for neurological disorders.
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| first_indexed | 2024-03-13T08:00:11Z |
| format | Article |
| id | doaj.art-7a757b838a244f3f98af13aa314565bd |
| institution | Directory Open Access Journal |
| issn | 2078-8665 2411-7986 |
| language | Arabic |
| last_indexed | 2024-03-13T08:00:11Z |
| publishDate | 2023-06-01 |
| publisher | College of Science for Women, University of Baghdad |
| record_format | Article |
| series | Baghdad Science Journal |
| spelling | doaj.art-7a757b838a244f3f98af13aa314565bd2023-06-01T19:45:01ZaraCollege of Science for Women, University of BaghdadBaghdad Science Journal2078-86652411-79862023-06-0120310.21123/bsj.2022.7280In vitro isolation and expansion of neural stem cells NSCsMaeda H. Mohammad 0Aous Kahtan Almzaien1Ahmad A. Al-Joubory2Ahmed M. Al-Shammari3Ayser A. Ahmed4Hiba K. Shaker5Aseel K. Abedalsattar6Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetics Research, Al-Mustansiriyah University, Baghdad, Iraq.Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetics Research, Al-Mustansiriyah University, Baghdad, Iraq.Neuroscience Hospital, Baghdad, Iraq. Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetics Research, Al-Mustansiriyah University, Baghdad, Iraq.Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetics Research, Al-Mustansiriyah University, Baghdad, Iraq.Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetics Research, Al-Mustansiriyah University, Baghdad, Iraq.Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetics Research, Al-Mustansiriyah University, Baghdad, Iraq. Neural stem cells (NSCs) are progenitor cells which have the ability to self‑renewal and potential for differentiating into neurons, oligodendrocytes, and astrocytes. The in vitro isolation, culturing, identification, cryopreservation were investigated to produce neural stem cells in culture as successful sources for further studies before using it for clinical trials. In this study, mouse bone marrow was the source of neural stem cells. The results of morphological study and immunocytochemistry of isolated cells showed that NSCs can be produced successfully and maintaining their self‑renewal and successfully forming neurosphere for multiple passages. The spheres preserved their morphology in culture and cryopreserved to be a ready source for use in experiments as a model for neurological disorders. https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7280Cryopreservation, Isolation, Nestin, Neural stem cells, Neurosphere, Stem cells |
| spellingShingle | Maeda H. Mohammad Aous Kahtan Almzaien Ahmad A. Al-Joubory Ahmed M. Al-Shammari Ayser A. Ahmed Hiba K. Shaker Aseel K. Abedalsattar In vitro isolation and expansion of neural stem cells NSCs Baghdad Science Journal Cryopreservation, Isolation, Nestin, Neural stem cells, Neurosphere, Stem cells |
| title | In vitro isolation and expansion of neural stem cells NSCs |
| title_full | In vitro isolation and expansion of neural stem cells NSCs |
| title_fullStr | In vitro isolation and expansion of neural stem cells NSCs |
| title_full_unstemmed | In vitro isolation and expansion of neural stem cells NSCs |
| title_short | In vitro isolation and expansion of neural stem cells NSCs |
| title_sort | in vitro isolation and expansion of neural stem cells nscs |
| topic | Cryopreservation, Isolation, Nestin, Neural stem cells, Neurosphere, Stem cells |
| url | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7280 |
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