Biochar stimulates tomato roots to recruit a bacterial assemblage contributing to disease resistance against Fusarium wilt
Abstract Biochar amendment is acknowledged to favor plant resistance against soil‐borne diseases. Although plant‐beneficial bacteria enrichment in the rhizosphere is often proposed to be associated with this protection, the mechanism behind this stimulating effect remains unelucidated. Here, we test...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-09-01
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| Series: | iMeta |
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| Online Access: | https://doi.org/10.1002/imt2.37 |
| _version_ | 1798029441410007040 |
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| author | Xue Jin Yang Bai Muhammad Khashi u Rahman Xiaojun Kang Kai Pan Fengzhi Wu Thomas Pommier Xingang Zhou Zhong Wei |
| author_facet | Xue Jin Yang Bai Muhammad Khashi u Rahman Xiaojun Kang Kai Pan Fengzhi Wu Thomas Pommier Xingang Zhou Zhong Wei |
| author_sort | Xue Jin |
| collection | DOAJ |
| description | Abstract Biochar amendment is acknowledged to favor plant resistance against soil‐borne diseases. Although plant‐beneficial bacteria enrichment in the rhizosphere is often proposed to be associated with this protection, the mechanism behind this stimulating effect remains unelucidated. Here, we tested whether biochar promotes plants to recruit beneficial bacteria to the rhizosphere, and thus develop a disease‐suppressive rhizosphere microbiome. In a pot experiment, biochar amendment decreased tomato Fusarium wilt disease severity. Using a transplanting rhizosphere microbiome experiment, we showed that biochar enhanced the suppressiveness of tomato rhizosphere microbiome against Fusarium wilt disease. High‐throughput sequencing of 16S ribosomal RNA gene and in vitro cultures further indicated that the recruited suppressive rhizosphere microbiome was associated with the increase of plant‐beneficial bacteria, such as Pseudomonas sp. This amendment also enhanced the in vitro chemoattraction and biofilm promotion activity of tomato root exudates. Collectively, our results demonstrate that biochar amendment induces tomato seedlings to efficiently recruit a disease‐suppressive rhizosphere microbiome against Fusarium wilt. |
| first_indexed | 2024-04-11T19:25:25Z |
| format | Article |
| id | doaj.art-7a78d1eb415948788197949a0c9e1604 |
| institution | Directory Open Access Journal |
| issn | 2770-596X |
| language | English |
| last_indexed | 2024-04-11T19:25:25Z |
| publishDate | 2022-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | iMeta |
| spelling | doaj.art-7a78d1eb415948788197949a0c9e16042022-12-22T04:07:11ZengWileyiMeta2770-596X2022-09-0113n/an/a10.1002/imt2.37Biochar stimulates tomato roots to recruit a bacterial assemblage contributing to disease resistance against Fusarium wiltXue Jin0Yang Bai1Muhammad Khashi u Rahman2Xiaojun Kang3Kai Pan4Fengzhi Wu5Thomas Pommier6Xingang Zhou7Zhong Wei8Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Department of Horticulture Northeast Agricultural University Harbin ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Department of Horticulture Northeast Agricultural University Harbin ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Department of Horticulture Northeast Agricultural University Harbin ChinaDepartment of Plant & Microbial Biology University of Minnesota Saint Paul Minnesota USAKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Department of Horticulture Northeast Agricultural University Harbin ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Department of Horticulture Northeast Agricultural University Harbin ChinaUniv Lyon, Université Claude Bernard Lyon 1, CNRS, INRAE, VetAgro Sup, UMR Ecologie Microbienne Villeurbanne FranceKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Department of Horticulture Northeast Agricultural University Harbin ChinaJiangsu Provincial Key Lab for Organic Solid Waste Utilization, Laboratory of Bio‐interactions and Crop Health, National Engineering Research Center for Organic‐based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Nanjing Agricultural University Nanjing ChinaAbstract Biochar amendment is acknowledged to favor plant resistance against soil‐borne diseases. Although plant‐beneficial bacteria enrichment in the rhizosphere is often proposed to be associated with this protection, the mechanism behind this stimulating effect remains unelucidated. Here, we tested whether biochar promotes plants to recruit beneficial bacteria to the rhizosphere, and thus develop a disease‐suppressive rhizosphere microbiome. In a pot experiment, biochar amendment decreased tomato Fusarium wilt disease severity. Using a transplanting rhizosphere microbiome experiment, we showed that biochar enhanced the suppressiveness of tomato rhizosphere microbiome against Fusarium wilt disease. High‐throughput sequencing of 16S ribosomal RNA gene and in vitro cultures further indicated that the recruited suppressive rhizosphere microbiome was associated with the increase of plant‐beneficial bacteria, such as Pseudomonas sp. This amendment also enhanced the in vitro chemoattraction and biofilm promotion activity of tomato root exudates. Collectively, our results demonstrate that biochar amendment induces tomato seedlings to efficiently recruit a disease‐suppressive rhizosphere microbiome against Fusarium wilt.https://doi.org/10.1002/imt2.37bacterial diversitydisease suppressionFusarium wiltmicrobiome recruitment |
| spellingShingle | Xue Jin Yang Bai Muhammad Khashi u Rahman Xiaojun Kang Kai Pan Fengzhi Wu Thomas Pommier Xingang Zhou Zhong Wei Biochar stimulates tomato roots to recruit a bacterial assemblage contributing to disease resistance against Fusarium wilt iMeta bacterial diversity disease suppression Fusarium wilt microbiome recruitment |
| title | Biochar stimulates tomato roots to recruit a bacterial assemblage contributing to disease resistance against Fusarium wilt |
| title_full | Biochar stimulates tomato roots to recruit a bacterial assemblage contributing to disease resistance against Fusarium wilt |
| title_fullStr | Biochar stimulates tomato roots to recruit a bacterial assemblage contributing to disease resistance against Fusarium wilt |
| title_full_unstemmed | Biochar stimulates tomato roots to recruit a bacterial assemblage contributing to disease resistance against Fusarium wilt |
| title_short | Biochar stimulates tomato roots to recruit a bacterial assemblage contributing to disease resistance against Fusarium wilt |
| title_sort | biochar stimulates tomato roots to recruit a bacterial assemblage contributing to disease resistance against fusarium wilt |
| topic | bacterial diversity disease suppression Fusarium wilt microbiome recruitment |
| url | https://doi.org/10.1002/imt2.37 |
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