Small Auxin Up RNAs influence the distribution of indole-3-acetic acid and play a potential role in increasing seed size in Euryale ferox Salisb

Abstract Background Aquatic Euryale ferox Salisb. is an economically important crop in China and India. Unfortunately, low yield limitations seriously hinder market growth. Unveiling the control of seed size is of remarkable importance in improvement of crops. Here, we generated a new hybrid line (H...

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Main Authors: Zhiheng Huang, Ke Bao, Zonghui Jing, Qian Wang, Huifang Duan, Yaying Zhu, Sen Zhang, Qinan Wu
Format: Article
Language:English
Published: BMC 2020-07-01
Series:BMC Plant Biology
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12870-020-02504-2
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author Zhiheng Huang
Ke Bao
Zonghui Jing
Qian Wang
Huifang Duan
Yaying Zhu
Sen Zhang
Qinan Wu
author_facet Zhiheng Huang
Ke Bao
Zonghui Jing
Qian Wang
Huifang Duan
Yaying Zhu
Sen Zhang
Qinan Wu
author_sort Zhiheng Huang
collection DOAJ
description Abstract Background Aquatic Euryale ferox Salisb. is an economically important crop in China and India. Unfortunately, low yield limitations seriously hinder market growth. Unveiling the control of seed size is of remarkable importance in improvement of crops. Here, we generated a new hybrid line (HL) with larger seeds by crossing South Gordon Euryale and North Gordon Euryale (WT) which hasn’t been reported before. However, the functional genes and molecular mechanisms controlling the seed size in Euryale ferox Salisb. remain unclear. In this study, we focused on the differentially expressed genes in the auxin signal transduction pathway during fruit development between HL and WT to explore candidate regulatory genes participated in regulating seed size. Results Both concentration and localization of indole-3-acetic acid (IAA) at two growth stages of fruits of WT and HL were detected by LC-MS and immunofluorescence. Although IAA content between the two lines did not differ, IAA distribution was significantly different. To elucidate the mechanism and to seek the key genes underlying this difference, RNA-seq was performed on young fruits at the two selected growth stages, and differentially expressed genes related to the auxin transduction pathway were selected for further analysis. Conclusion Hybrid Euryale ferox Salisb. expressed significant heterosis, resulting in non-prickly, thin-coated, large seeds, which accounted for the significantly larger yield of HL than that of WT. Our study indicated that Small Auxin Up RNAs (SAURs) -mediated localization of IAA regulates seed size in Euryale ferox Salisb. We found that some SAURs may act as a positive mediator of the auxin transduction pathway, thereby contributing to the observed heterosis.
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spelling doaj.art-7a7e361548ce45a2b7660a27e0086fa62022-12-21T18:11:48ZengBMCBMC Plant Biology1471-22292020-07-0120111310.1186/s12870-020-02504-2Small Auxin Up RNAs influence the distribution of indole-3-acetic acid and play a potential role in increasing seed size in Euryale ferox SalisbZhiheng Huang0Ke Bao1Zonghui Jing2Qian Wang3Huifang Duan4Yaying Zhu5Sen Zhang6Qinan Wu7School of Pharmacy, Nanjing University of Chinese MedicineSchool of Pharmacy, Nanjing University of Chinese MedicineSchool of Pharmacy, Nanjing University of Chinese MedicineSchool of Pharmacy, Nanjing University of Chinese MedicineSchool of Pharmacy, Nanjing University of Chinese MedicineSchool of Pharmacy, Nanjing University of Chinese MedicineSchool of Pharmacy, Nanjing University of Chinese MedicineSchool of Pharmacy, Nanjing University of Chinese MedicineAbstract Background Aquatic Euryale ferox Salisb. is an economically important crop in China and India. Unfortunately, low yield limitations seriously hinder market growth. Unveiling the control of seed size is of remarkable importance in improvement of crops. Here, we generated a new hybrid line (HL) with larger seeds by crossing South Gordon Euryale and North Gordon Euryale (WT) which hasn’t been reported before. However, the functional genes and molecular mechanisms controlling the seed size in Euryale ferox Salisb. remain unclear. In this study, we focused on the differentially expressed genes in the auxin signal transduction pathway during fruit development between HL and WT to explore candidate regulatory genes participated in regulating seed size. Results Both concentration and localization of indole-3-acetic acid (IAA) at two growth stages of fruits of WT and HL were detected by LC-MS and immunofluorescence. Although IAA content between the two lines did not differ, IAA distribution was significantly different. To elucidate the mechanism and to seek the key genes underlying this difference, RNA-seq was performed on young fruits at the two selected growth stages, and differentially expressed genes related to the auxin transduction pathway were selected for further analysis. Conclusion Hybrid Euryale ferox Salisb. expressed significant heterosis, resulting in non-prickly, thin-coated, large seeds, which accounted for the significantly larger yield of HL than that of WT. Our study indicated that Small Auxin Up RNAs (SAURs) -mediated localization of IAA regulates seed size in Euryale ferox Salisb. We found that some SAURs may act as a positive mediator of the auxin transduction pathway, thereby contributing to the observed heterosis.http://link.springer.com/article/10.1186/s12870-020-02504-2Euryale ferox SalisbHybridRNA-seqIAASAUR
spellingShingle Zhiheng Huang
Ke Bao
Zonghui Jing
Qian Wang
Huifang Duan
Yaying Zhu
Sen Zhang
Qinan Wu
Small Auxin Up RNAs influence the distribution of indole-3-acetic acid and play a potential role in increasing seed size in Euryale ferox Salisb
BMC Plant Biology
Euryale ferox Salisb
Hybrid
RNA-seq
IAA
SAUR
title Small Auxin Up RNAs influence the distribution of indole-3-acetic acid and play a potential role in increasing seed size in Euryale ferox Salisb
title_full Small Auxin Up RNAs influence the distribution of indole-3-acetic acid and play a potential role in increasing seed size in Euryale ferox Salisb
title_fullStr Small Auxin Up RNAs influence the distribution of indole-3-acetic acid and play a potential role in increasing seed size in Euryale ferox Salisb
title_full_unstemmed Small Auxin Up RNAs influence the distribution of indole-3-acetic acid and play a potential role in increasing seed size in Euryale ferox Salisb
title_short Small Auxin Up RNAs influence the distribution of indole-3-acetic acid and play a potential role in increasing seed size in Euryale ferox Salisb
title_sort small auxin up rnas influence the distribution of indole 3 acetic acid and play a potential role in increasing seed size in euryale ferox salisb
topic Euryale ferox Salisb
Hybrid
RNA-seq
IAA
SAUR
url http://link.springer.com/article/10.1186/s12870-020-02504-2
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