Phytol-Loaded Solid Lipid Nanoparticles as a Novel Anticandidal Nanobiotechnological Approach
Phytol is a diterpene alcohol and can be found as a product of the metabolism of chlorophyll in plants. This compound has been explored as a potential antimicrobial agent, but it is insoluble in water. In this study, we describe a novel approach for an interesting anticandidal drug delivery system c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/12/9/871 |
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| author | Tábata L. C. Lima Luanda B. F. C. Souza Lannya C. S. Tavares-Pessoa Alaine M. dos Santos-Silva Rômulo S. Cavalcante Raimundo F. de Araújo-Júnior Alianda M. Cornélio Matheus F. Fernandes-Pedrosa Guilherme Maranhão Chaves Arnóbio Antônio da Silva-Júnior |
| author_facet | Tábata L. C. Lima Luanda B. F. C. Souza Lannya C. S. Tavares-Pessoa Alaine M. dos Santos-Silva Rômulo S. Cavalcante Raimundo F. de Araújo-Júnior Alianda M. Cornélio Matheus F. Fernandes-Pedrosa Guilherme Maranhão Chaves Arnóbio Antônio da Silva-Júnior |
| author_sort | Tábata L. C. Lima |
| collection | DOAJ |
| description | Phytol is a diterpene alcohol and can be found as a product of the metabolism of chlorophyll in plants. This compound has been explored as a potential antimicrobial agent, but it is insoluble in water. In this study, we describe a novel approach for an interesting anticandidal drug delivery system containing phytol. Different formulations of phytol-loaded solid lipid nanoparticles (SLN) were designed and tested using a natural lipid, 1,3-distearyl-2-oleyl-glycerol (TG1). Different compositions were considered to obtain three formulations with 1:10, 1:5, and 1:3 <i>w/w</i> phytol/TG1 ratios. All the formulations were prepared by emulsification solvent evaporation method and had their physicochemical properties assessed. The biocompatibility assay was performed in the HEK-293 cell line and the antifungal efficacy was demonstrated in different strains of <i>Candida</i> ssp., including different clinical isolates. Spherical and uniform SLN (<300 nm, PdI < 0.2) with phytol-loading efficiency >65% were achieved. Phytol-loaded SLN showed a dose-dependent cytotoxic effect in the HEK-293 cell line. The three tested formulations of phytol-loaded SLN considerably enhanced the minimal inhibitory concentration of phytol against 15 strains of <i>Candida</i> spp. Considering the clinical isolates, the formulations containing the highest phytol/TG1 ratios showed MICs at 100%. Thus, the feasibility and potential of phytol-loaded SLN was demonstrated in vitro, being a promising nanocarrier for phytol delivery from an anticandidal approach. |
| first_indexed | 2024-03-10T16:21:36Z |
| format | Article |
| id | doaj.art-7a7f465cd3d74b5f9fabfe481788b05d |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T16:21:36Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-7a7f465cd3d74b5f9fabfe481788b05d2023-11-20T13:35:01ZengMDPI AGPharmaceutics1999-49232020-09-0112987110.3390/pharmaceutics12090871Phytol-Loaded Solid Lipid Nanoparticles as a Novel Anticandidal Nanobiotechnological ApproachTábata L. C. Lima0Luanda B. F. C. Souza1Lannya C. S. Tavares-Pessoa2Alaine M. dos Santos-Silva3Rômulo S. Cavalcante4Raimundo F. de Araújo-Júnior5Alianda M. Cornélio6Matheus F. Fernandes-Pedrosa7Guilherme Maranhão Chaves8Arnóbio Antônio da Silva-Júnior9Laboratory of Pharmaceutical Technology and Biotechnology, Department of Pharmacy, Federal University of Rio Grande do Norte (UFRN), Gal. Gustavo Cordeiro de Farias, S/N, Petrópolis, Natal-RN 59072-570, BrazilDepartment of Clinical and Toxicological Analyses, Federal University of Rio Grande do Norte (UFRN), Gal, Gustavo Cordeiro de Faria, S/N, Petrópolis, Natal-RN 59084-100, BrazilLaboratory of Pharmaceutical Technology and Biotechnology, Department of Pharmacy, Federal University of Rio Grande do Norte (UFRN), Gal. Gustavo Cordeiro de Farias, S/N, Petrópolis, Natal-RN 59072-570, BrazilLaboratory of Pharmaceutical Technology and Biotechnology, Department of Pharmacy, Federal University of Rio Grande do Norte (UFRN), Gal. Gustavo Cordeiro de Farias, S/N, Petrópolis, Natal-RN 59072-570, BrazilDepartment of Morphology, Federal University of Rio Grande do Norte (UFRN), Natal-RN 59.078-970, BrazilDepartment of Morphology, Federal University of Rio Grande do Norte (UFRN), Natal-RN 59.078-970, BrazilDepartment of Morphology, Federal University of Rio Grande do Norte (UFRN), Natal-RN 59.078-970, BrazilLaboratory of Pharmaceutical Technology and Biotechnology, Department of Pharmacy, Federal University of Rio Grande do Norte (UFRN), Gal. Gustavo Cordeiro de Farias, S/N, Petrópolis, Natal-RN 59072-570, BrazilDepartment of Clinical and Toxicological Analyses, Federal University of Rio Grande do Norte (UFRN), Gal, Gustavo Cordeiro de Faria, S/N, Petrópolis, Natal-RN 59084-100, BrazilLaboratory of Pharmaceutical Technology and Biotechnology, Department of Pharmacy, Federal University of Rio Grande do Norte (UFRN), Gal. Gustavo Cordeiro de Farias, S/N, Petrópolis, Natal-RN 59072-570, BrazilPhytol is a diterpene alcohol and can be found as a product of the metabolism of chlorophyll in plants. This compound has been explored as a potential antimicrobial agent, but it is insoluble in water. In this study, we describe a novel approach for an interesting anticandidal drug delivery system containing phytol. Different formulations of phytol-loaded solid lipid nanoparticles (SLN) were designed and tested using a natural lipid, 1,3-distearyl-2-oleyl-glycerol (TG1). Different compositions were considered to obtain three formulations with 1:10, 1:5, and 1:3 <i>w/w</i> phytol/TG1 ratios. All the formulations were prepared by emulsification solvent evaporation method and had their physicochemical properties assessed. The biocompatibility assay was performed in the HEK-293 cell line and the antifungal efficacy was demonstrated in different strains of <i>Candida</i> ssp., including different clinical isolates. Spherical and uniform SLN (<300 nm, PdI < 0.2) with phytol-loading efficiency >65% were achieved. Phytol-loaded SLN showed a dose-dependent cytotoxic effect in the HEK-293 cell line. The three tested formulations of phytol-loaded SLN considerably enhanced the minimal inhibitory concentration of phytol against 15 strains of <i>Candida</i> spp. Considering the clinical isolates, the formulations containing the highest phytol/TG1 ratios showed MICs at 100%. Thus, the feasibility and potential of phytol-loaded SLN was demonstrated in vitro, being a promising nanocarrier for phytol delivery from an anticandidal approach.https://www.mdpi.com/1999-4923/12/9/871solid lipid nanoparticlesphytol<i>Candida</i> spp.1,3-distearyl-2-oleyl-glycerol (TG1)anticandidal activity |
| spellingShingle | Tábata L. C. Lima Luanda B. F. C. Souza Lannya C. S. Tavares-Pessoa Alaine M. dos Santos-Silva Rômulo S. Cavalcante Raimundo F. de Araújo-Júnior Alianda M. Cornélio Matheus F. Fernandes-Pedrosa Guilherme Maranhão Chaves Arnóbio Antônio da Silva-Júnior Phytol-Loaded Solid Lipid Nanoparticles as a Novel Anticandidal Nanobiotechnological Approach Pharmaceutics solid lipid nanoparticles phytol <i>Candida</i> spp. 1,3-distearyl-2-oleyl-glycerol (TG1) anticandidal activity |
| title | Phytol-Loaded Solid Lipid Nanoparticles as a Novel Anticandidal Nanobiotechnological Approach |
| title_full | Phytol-Loaded Solid Lipid Nanoparticles as a Novel Anticandidal Nanobiotechnological Approach |
| title_fullStr | Phytol-Loaded Solid Lipid Nanoparticles as a Novel Anticandidal Nanobiotechnological Approach |
| title_full_unstemmed | Phytol-Loaded Solid Lipid Nanoparticles as a Novel Anticandidal Nanobiotechnological Approach |
| title_short | Phytol-Loaded Solid Lipid Nanoparticles as a Novel Anticandidal Nanobiotechnological Approach |
| title_sort | phytol loaded solid lipid nanoparticles as a novel anticandidal nanobiotechnological approach |
| topic | solid lipid nanoparticles phytol <i>Candida</i> spp. 1,3-distearyl-2-oleyl-glycerol (TG1) anticandidal activity |
| url | https://www.mdpi.com/1999-4923/12/9/871 |
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