Biocontrol of Fusarium equiseti using chitosan nanoparticles combined with Trichoderma longibrachiatum and Penicillium polonicum
Abstract A safe and ecofriendly biocontrol of pathogenic Fusarium equiseti was developed based on chitosan nanoparticles (CNPs) combined with Trichoderma longibrachiatum and Penicillium polonicum. Two strains of F. equiseti which were isolated from wilting tomato plant as well as three antagonistic...
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | Fungal Biology and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40694-023-00151-4 |
| _version_ | 1811166066384044032 |
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| author | EL-Sayed M. El-Morsy Yomna S. Elmalahy Mohamed M. A. Mousa |
| author_facet | EL-Sayed M. El-Morsy Yomna S. Elmalahy Mohamed M. A. Mousa |
| author_sort | EL-Sayed M. El-Morsy |
| collection | DOAJ |
| description | Abstract A safe and ecofriendly biocontrol of pathogenic Fusarium equiseti was developed based on chitosan nanoparticles (CNPs) combined with Trichoderma longibrachiatum and Penicillium polonicum. Two strains of F. equiseti which were isolated from wilting tomato plant as well as three antagonistic fungi including Trichoderma longibrachiatum and two strains of Penicillium polonicum were isolated from the surrounding soil. All the isolated pathogenic and antagonistic fungi were identified using genomic DNA sequences. The antifungal activity of the three antagonistic fungi were studied against the two strains of F. equiseti. Also, CNPs which were prepared according to the ionic gelation method using sodium tripolyphosphate anions in acetic acid solution were used to enhance the antifungal activity of the three antagonistic fungi. The results exhibit that, combination of T. longibrachiatum with CNPs and P. polonicum with CNPs achieve high antifungal activity against F. equiseti by an inhibition rate equal to 71.05% and 66.7%, respectively. |
| first_indexed | 2024-04-10T15:46:28Z |
| format | Article |
| id | doaj.art-7a7f87bd9310422f9bb7b9fcc496c019 |
| institution | Directory Open Access Journal |
| issn | 2054-3085 |
| language | English |
| last_indexed | 2024-04-10T15:46:28Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Fungal Biology and Biotechnology |
| spelling | doaj.art-7a7f87bd9310422f9bb7b9fcc496c0192023-02-12T12:03:32ZengBMCFungal Biology and Biotechnology2054-30852023-02-011011810.1186/s40694-023-00151-4Biocontrol of Fusarium equiseti using chitosan nanoparticles combined with Trichoderma longibrachiatum and Penicillium polonicumEL-Sayed M. El-Morsy0Yomna S. Elmalahy1Mohamed M. A. Mousa2Botany and Microbiology Department, Faculty of Science, Damietta UniversityBotany and Microbiology Department, Faculty of Science, Damietta UniversityBotany and Microbiology Department, Faculty of Science, Damietta UniversityAbstract A safe and ecofriendly biocontrol of pathogenic Fusarium equiseti was developed based on chitosan nanoparticles (CNPs) combined with Trichoderma longibrachiatum and Penicillium polonicum. Two strains of F. equiseti which were isolated from wilting tomato plant as well as three antagonistic fungi including Trichoderma longibrachiatum and two strains of Penicillium polonicum were isolated from the surrounding soil. All the isolated pathogenic and antagonistic fungi were identified using genomic DNA sequences. The antifungal activity of the three antagonistic fungi were studied against the two strains of F. equiseti. Also, CNPs which were prepared according to the ionic gelation method using sodium tripolyphosphate anions in acetic acid solution were used to enhance the antifungal activity of the three antagonistic fungi. The results exhibit that, combination of T. longibrachiatum with CNPs and P. polonicum with CNPs achieve high antifungal activity against F. equiseti by an inhibition rate equal to 71.05% and 66.7%, respectively.https://doi.org/10.1186/s40694-023-00151-4Fusarium equisetiChitosan nanoparticlesAntifungalPathogenic fungiAntagonistic fungi |
| spellingShingle | EL-Sayed M. El-Morsy Yomna S. Elmalahy Mohamed M. A. Mousa Biocontrol of Fusarium equiseti using chitosan nanoparticles combined with Trichoderma longibrachiatum and Penicillium polonicum Fungal Biology and Biotechnology Fusarium equiseti Chitosan nanoparticles Antifungal Pathogenic fungi Antagonistic fungi |
| title | Biocontrol of Fusarium equiseti using chitosan nanoparticles combined with Trichoderma longibrachiatum and Penicillium polonicum |
| title_full | Biocontrol of Fusarium equiseti using chitosan nanoparticles combined with Trichoderma longibrachiatum and Penicillium polonicum |
| title_fullStr | Biocontrol of Fusarium equiseti using chitosan nanoparticles combined with Trichoderma longibrachiatum and Penicillium polonicum |
| title_full_unstemmed | Biocontrol of Fusarium equiseti using chitosan nanoparticles combined with Trichoderma longibrachiatum and Penicillium polonicum |
| title_short | Biocontrol of Fusarium equiseti using chitosan nanoparticles combined with Trichoderma longibrachiatum and Penicillium polonicum |
| title_sort | biocontrol of fusarium equiseti using chitosan nanoparticles combined with trichoderma longibrachiatum and penicillium polonicum |
| topic | Fusarium equiseti Chitosan nanoparticles Antifungal Pathogenic fungi Antagonistic fungi |
| url | https://doi.org/10.1186/s40694-023-00151-4 |
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