Effects of Usnic Acid on Apoptosis and Expression of Bax and Bcl-2 Proteins in Hippocampal CA1 Neurons Following Cerebral Ischemia-Reperfusion
Introduction: Cerebral ischemia-reperfusion causes complex pathological mechanisms that lead to tissue damage, such as neuronal apoptosis. Usnic acid is a secondary metabolite of lichen and has various biological properties including antioxidant and anti-inflammatory activities. This study aimed to...
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| Format: | Article |
| Language: | fas |
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Ilam University of Medical Sciences
2022-08-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
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| Online Access: | http://sjimu.medilam.ac.ir/article-1-7149-en.html |
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| author | Sohaila Erfani Tahereh Valadbeigi Mehdi Khaksari Ali Moghimi Nahid Aboutaleb |
| author_facet | Sohaila Erfani Tahereh Valadbeigi Mehdi Khaksari Ali Moghimi Nahid Aboutaleb |
| author_sort | Sohaila Erfani |
| collection | DOAJ |
| description | Introduction: Cerebral ischemia-reperfusion causes complex pathological mechanisms that lead to tissue damage, such as neuronal apoptosis. Usnic acid is a secondary metabolite of lichen and has various biological properties including antioxidant and anti-inflammatory activities. This study aimed to investigate the neuroprotective effects of usnic acid on apoptotic cell death and apoptotic-related proteins (Bax and Bcl-2) in the CA1 area of the hippocampus following cerebral ischemia-reperfusion.
Material & Methods: A total of 42 male Wistar rats were randomly divided into three groups (sham, ischemia-reperfusion, and ischemia-reperfusion+usnic acid). Ischemia was induced by occlusion of both common carotid arteries for 20 min. Usnic acid (25 mg/kg, intraperitoneally) was injected at the beginning of reperfusion time. The expression levels of Bax and Bcl-2 proteins were measured using the immunofluorescence method, and the rate of apoptotic cell death was determined using the TUNEL staining method.
(Ethic code: IR.SHMU.REC.1397.186)
Findings: In this study, cerebral ischemia increased apoptotic cell death in the CA1 area of the hippocampus. It was demonstrated that usnic acid significantly decreased apoptotic cell death by decreasing the expression of Bax pro-apoptotic protein and increasing the expression of Bcl-2 (anti-apoptotic protein).
Discussion & Conclusion: It can be concluded that usnic acid has a neuroprotective effect against cerebral ischemia-reperfusion damages by reducing cell death. |
| first_indexed | 2024-04-12T01:36:18Z |
| format | Article |
| id | doaj.art-7a837cb78e8a4ee29ff071c5f7e2311c |
| institution | Directory Open Access Journal |
| issn | 1563-4728 2588-3135 |
| language | fas |
| last_indexed | 2024-04-12T01:36:18Z |
| publishDate | 2022-08-01 |
| publisher | Ilam University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| spelling | doaj.art-7a837cb78e8a4ee29ff071c5f7e2311c2022-12-22T03:53:18ZfasIlam University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām1563-47282588-31352022-08-01303101111Effects of Usnic Acid on Apoptosis and Expression of Bax and Bcl-2 Proteins in Hippocampal CA1 Neurons Following Cerebral Ischemia-ReperfusionSohaila Erfani0Tahereh Valadbeigi1Mehdi Khaksari2Ali Moghimi3Nahid Aboutaleb4 Dept of Biology, Faculty of Science, Ilam University, Ilam, Iran Dept of Biology, Faculty of Science, Ilam University, Ilam, Iran Faculty of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran Rayan Research Center for Neuroscience and Behavior, Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran Physiology Research Center, Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Introduction: Cerebral ischemia-reperfusion causes complex pathological mechanisms that lead to tissue damage, such as neuronal apoptosis. Usnic acid is a secondary metabolite of lichen and has various biological properties including antioxidant and anti-inflammatory activities. This study aimed to investigate the neuroprotective effects of usnic acid on apoptotic cell death and apoptotic-related proteins (Bax and Bcl-2) in the CA1 area of the hippocampus following cerebral ischemia-reperfusion. Material & Methods: A total of 42 male Wistar rats were randomly divided into three groups (sham, ischemia-reperfusion, and ischemia-reperfusion+usnic acid). Ischemia was induced by occlusion of both common carotid arteries for 20 min. Usnic acid (25 mg/kg, intraperitoneally) was injected at the beginning of reperfusion time. The expression levels of Bax and Bcl-2 proteins were measured using the immunofluorescence method, and the rate of apoptotic cell death was determined using the TUNEL staining method. (Ethic code: IR.SHMU.REC.1397.186) Findings: In this study, cerebral ischemia increased apoptotic cell death in the CA1 area of the hippocampus. It was demonstrated that usnic acid significantly decreased apoptotic cell death by decreasing the expression of Bax pro-apoptotic protein and increasing the expression of Bcl-2 (anti-apoptotic protein). Discussion & Conclusion: It can be concluded that usnic acid has a neuroprotective effect against cerebral ischemia-reperfusion damages by reducing cell death.http://sjimu.medilam.ac.ir/article-1-7149-en.htmlapoptosishippocampusischemia-reperfusionusnic acid |
| spellingShingle | Sohaila Erfani Tahereh Valadbeigi Mehdi Khaksari Ali Moghimi Nahid Aboutaleb Effects of Usnic Acid on Apoptosis and Expression of Bax and Bcl-2 Proteins in Hippocampal CA1 Neurons Following Cerebral Ischemia-Reperfusion Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām apoptosis hippocampus ischemia-reperfusion usnic acid |
| title | Effects of Usnic Acid on Apoptosis and Expression of Bax and Bcl-2 Proteins in Hippocampal CA1 Neurons Following Cerebral Ischemia-Reperfusion |
| title_full | Effects of Usnic Acid on Apoptosis and Expression of Bax and Bcl-2 Proteins in Hippocampal CA1 Neurons Following Cerebral Ischemia-Reperfusion |
| title_fullStr | Effects of Usnic Acid on Apoptosis and Expression of Bax and Bcl-2 Proteins in Hippocampal CA1 Neurons Following Cerebral Ischemia-Reperfusion |
| title_full_unstemmed | Effects of Usnic Acid on Apoptosis and Expression of Bax and Bcl-2 Proteins in Hippocampal CA1 Neurons Following Cerebral Ischemia-Reperfusion |
| title_short | Effects of Usnic Acid on Apoptosis and Expression of Bax and Bcl-2 Proteins in Hippocampal CA1 Neurons Following Cerebral Ischemia-Reperfusion |
| title_sort | effects of usnic acid on apoptosis and expression of bax and bcl 2 proteins in hippocampal ca1 neurons following cerebral ischemia reperfusion |
| topic | apoptosis hippocampus ischemia-reperfusion usnic acid |
| url | http://sjimu.medilam.ac.ir/article-1-7149-en.html |
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