Examination of equine glandular stomach lesions for bacteria, including <it>Helicobacter spp </it>by fluorescence <it>in situ </it>hybridisation
<p>Abstract</p> <p>Background</p> <p>The equine glandular stomach is commonly affected by erosion and ulceration. The aim of this study was to assess whether bacteria, including Helicobacter, could be involved in the aetiology of gastric glandular lesions seen in horses...
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| Format: | Article |
| Language: | English |
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BMC
2010-03-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/10/84 |
| _version_ | 1811299283268272128 |
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| author | Olsen Susanne N Jensen Tim K Husted Louise Mølbak Lars |
| author_facet | Olsen Susanne N Jensen Tim K Husted Louise Mølbak Lars |
| author_sort | Olsen Susanne N |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The equine glandular stomach is commonly affected by erosion and ulceration. The aim of this study was to assess whether bacteria, including Helicobacter, could be involved in the aetiology of gastric glandular lesions seen in horses.</p> <p>Results</p> <p>Stomach lesions, as well as normal appearing mucosa were obtained from horses slaughtered for human consumption. All samples were tested for urease activity using the Pyloritek<sup>® </sup>assay, while mucosal bacterial content was evaluated using Fluorescence <it>In Situ </it>Hybridisation. In selected sub samples, bacteria characterisation was pursued further by cloning and sequencing. Mucosal lesions were found in 36/63 stomachs and included hyperplastic rugae, polypoid structures and focal erosions. None of the samples were tested positive for urease activity or for FISH using the Helicobacter genus specific probe. In samples of lesions, as well as normal samples, clones with 99% similarities to <it>Lactobacillus salivarius </it>and <it>Sarcina ventriculi </it>were found. <it>Escherichia </it>like bacterium clones and Enterococcus clones were demonstrated in one focal erosion. Based on a phylogenetic tree these clones had 100% similarity to <it>Escherichia fergusonii and Enterococcus faecium</it>. The Enterococcus were found colonising the mucosal surface, while <it>E. fergusonii </it>organisms were also demonstrated intraepithelial.</p> <p>Conclusion</p> <p>Gastric Helicobacter spp. could not be verified as being involved in lesions of the glandular stomach of the horse. Since <it>E. fergusonii </it>has been described as an emerging pathogen in both humans and animals, the finding of this bacterium in gastric erosion warrants further clarification to whether gastric infection with this type bacterium is important for horses.</p> |
| first_indexed | 2024-04-13T06:32:57Z |
| format | Article |
| id | doaj.art-7a85c72699054df1bf27a60c5d576920 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-13T06:32:57Z |
| publishDate | 2010-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-7a85c72699054df1bf27a60c5d5769202022-12-22T02:58:02ZengBMCBMC Microbiology1471-21802010-03-011018410.1186/1471-2180-10-84Examination of equine glandular stomach lesions for bacteria, including <it>Helicobacter spp </it>by fluorescence <it>in situ </it>hybridisationOlsen Susanne NJensen Tim KHusted LouiseMølbak Lars<p>Abstract</p> <p>Background</p> <p>The equine glandular stomach is commonly affected by erosion and ulceration. The aim of this study was to assess whether bacteria, including Helicobacter, could be involved in the aetiology of gastric glandular lesions seen in horses.</p> <p>Results</p> <p>Stomach lesions, as well as normal appearing mucosa were obtained from horses slaughtered for human consumption. All samples were tested for urease activity using the Pyloritek<sup>® </sup>assay, while mucosal bacterial content was evaluated using Fluorescence <it>In Situ </it>Hybridisation. In selected sub samples, bacteria characterisation was pursued further by cloning and sequencing. Mucosal lesions were found in 36/63 stomachs and included hyperplastic rugae, polypoid structures and focal erosions. None of the samples were tested positive for urease activity or for FISH using the Helicobacter genus specific probe. In samples of lesions, as well as normal samples, clones with 99% similarities to <it>Lactobacillus salivarius </it>and <it>Sarcina ventriculi </it>were found. <it>Escherichia </it>like bacterium clones and Enterococcus clones were demonstrated in one focal erosion. Based on a phylogenetic tree these clones had 100% similarity to <it>Escherichia fergusonii and Enterococcus faecium</it>. The Enterococcus were found colonising the mucosal surface, while <it>E. fergusonii </it>organisms were also demonstrated intraepithelial.</p> <p>Conclusion</p> <p>Gastric Helicobacter spp. could not be verified as being involved in lesions of the glandular stomach of the horse. Since <it>E. fergusonii </it>has been described as an emerging pathogen in both humans and animals, the finding of this bacterium in gastric erosion warrants further clarification to whether gastric infection with this type bacterium is important for horses.</p>http://www.biomedcentral.com/1471-2180/10/84 |
| spellingShingle | Olsen Susanne N Jensen Tim K Husted Louise Mølbak Lars Examination of equine glandular stomach lesions for bacteria, including <it>Helicobacter spp </it>by fluorescence <it>in situ </it>hybridisation BMC Microbiology |
| title | Examination of equine glandular stomach lesions for bacteria, including <it>Helicobacter spp </it>by fluorescence <it>in situ </it>hybridisation |
| title_full | Examination of equine glandular stomach lesions for bacteria, including <it>Helicobacter spp </it>by fluorescence <it>in situ </it>hybridisation |
| title_fullStr | Examination of equine glandular stomach lesions for bacteria, including <it>Helicobacter spp </it>by fluorescence <it>in situ </it>hybridisation |
| title_full_unstemmed | Examination of equine glandular stomach lesions for bacteria, including <it>Helicobacter spp </it>by fluorescence <it>in situ </it>hybridisation |
| title_short | Examination of equine glandular stomach lesions for bacteria, including <it>Helicobacter spp </it>by fluorescence <it>in situ </it>hybridisation |
| title_sort | examination of equine glandular stomach lesions for bacteria including it helicobacter spp it by fluorescence it in situ it hybridisation |
| url | http://www.biomedcentral.com/1471-2180/10/84 |
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