Polymorphism study of TLR4 gene in crossbred cattle of Kerala
The present study was conducted to identify single nucleotide variations in exon 3 region of the bovine Toll-Like Receptor gene (TLR4) associated with somatic cell score (SCS) in crossbred cattle of Kerala using polymerase chain reaction-single stranded conformational polymorphisms (PCR-SSCP) tec...
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Director of Academics and Research, Kerala Veterinary and Animal Sciences University
2023-03-01
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| Series: | Journal of Veterinary and Animal Sciences |
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| Online Access: | https://jvas.in/public_html/upload/article_file/article_file_rsdity.pdf?t=rsdity |
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| author | G. Chishi Kiyevi Valsalan Jamuna Sadan Tina K. Anilkumar T. V. Aravindakshan K. Radha |
| author_facet | G. Chishi Kiyevi Valsalan Jamuna Sadan Tina K. Anilkumar T. V. Aravindakshan K. Radha |
| author_sort | G. Chishi Kiyevi |
| collection | DOAJ |
| description | The present study was conducted to identify single nucleotide variations in exon 3 region
of the bovine Toll-Like Receptor gene (TLR4) associated with somatic cell score (SCS) in crossbred
cattle of Kerala using polymerase chain reaction-single stranded conformational polymorphisms
(PCR-SSCP) technique. Blood samples were collected from 200 lactating crossbred cattle in
different farms of Kerala Veterinary and Animal Sciences University (KVASU) and field centres of
ICAR- FPT scheme, KVASU. Genomic DNA was isolated and polymorphisms were identified by
SSCP analysis. The 231 bp fragment of exon 3 in TLR4 gene was found to polymorphic and two
genotypes viz. CC and CD were obtained with frequencies 77 per cent and 23 per cent respectively.
The frequencies of C and D allele in crossbred were found to be 0.88 and 0.12. On sequencing,
C to T transition had led to one non-synonymous change at 2021th position of ORF. Cattle with CC
genotype were associated with significantly lower SCS (p≤0.05) as compared to CD genotype. The
association between TLR4 polymorphism and SCS described in this study suggests the possible
role of SNP during mastitis infection. Thus, could be employed as a useful marker for genetic
selection to promote mastitis resistance in dairy cattle. |
| first_indexed | 2024-04-09T18:24:24Z |
| format | Article |
| id | doaj.art-7a89c3374a4249a9b948f536c15fe542 |
| institution | Directory Open Access Journal |
| issn | 0971-0701 2582-0605 |
| language | English |
| last_indexed | 2024-04-09T18:24:24Z |
| publishDate | 2023-03-01 |
| publisher | Director of Academics and Research, Kerala Veterinary and Animal Sciences University |
| record_format | Article |
| series | Journal of Veterinary and Animal Sciences |
| spelling | doaj.art-7a89c3374a4249a9b948f536c15fe5422023-04-12T05:06:02ZengDirector of Academics and Research, Kerala Veterinary and Animal Sciences UniversityJournal of Veterinary and Animal Sciences0971-07012582-06052023-03-015411610.51966/jvas.2023.54.1.1-6Polymorphism study of TLR4 gene in crossbred cattle of KeralaG. Chishi Kiyevi0https://orcid.org/0009-0002-8240-393XValsalan Jamuna 1https://orcid.org/0000-0002-6283-8955Sadan Tina2https://orcid.org/0000-0002-9352-0616K. Anilkumar3https://orcid.org/0000-0002-1763-7433T. V. Aravindakshan4https://orcid.org/0000-0002-5957-1516K. Radha5 MVSc Scholar, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Mannuthy, Thrissur.Assistant Professor, Centre for Advanced Studies in Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Mannuthy, Thrissur.Junior Research Fellow, DST-SERB, Centre for Advanced Studies in Animal Genetics and BreedingProfessor, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Mannuthy, ThrissurProfessor and Head, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Mannuthy, Thrissur.Assistant Professor, Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, Thrissur.The present study was conducted to identify single nucleotide variations in exon 3 region of the bovine Toll-Like Receptor gene (TLR4) associated with somatic cell score (SCS) in crossbred cattle of Kerala using polymerase chain reaction-single stranded conformational polymorphisms (PCR-SSCP) technique. Blood samples were collected from 200 lactating crossbred cattle in different farms of Kerala Veterinary and Animal Sciences University (KVASU) and field centres of ICAR- FPT scheme, KVASU. Genomic DNA was isolated and polymorphisms were identified by SSCP analysis. The 231 bp fragment of exon 3 in TLR4 gene was found to polymorphic and two genotypes viz. CC and CD were obtained with frequencies 77 per cent and 23 per cent respectively. The frequencies of C and D allele in crossbred were found to be 0.88 and 0.12. On sequencing, C to T transition had led to one non-synonymous change at 2021th position of ORF. Cattle with CC genotype were associated with significantly lower SCS (p≤0.05) as compared to CD genotype. The association between TLR4 polymorphism and SCS described in this study suggests the possible role of SNP during mastitis infection. Thus, could be employed as a useful marker for genetic selection to promote mastitis resistance in dairy cattle.https://jvas.in/public_html/upload/article_file/article_file_rsdity.pdf?t=rsditytlr4pcr-sscpsomatic cell scorecrossbred cattle |
| spellingShingle | G. Chishi Kiyevi Valsalan Jamuna Sadan Tina K. Anilkumar T. V. Aravindakshan K. Radha Polymorphism study of TLR4 gene in crossbred cattle of Kerala Journal of Veterinary and Animal Sciences tlr4 pcr-sscp somatic cell score crossbred cattle |
| title | Polymorphism study of TLR4 gene in crossbred cattle of Kerala |
| title_full | Polymorphism study of TLR4 gene in crossbred cattle of Kerala |
| title_fullStr | Polymorphism study of TLR4 gene in crossbred cattle of Kerala |
| title_full_unstemmed | Polymorphism study of TLR4 gene in crossbred cattle of Kerala |
| title_short | Polymorphism study of TLR4 gene in crossbred cattle of Kerala |
| title_sort | polymorphism study of tlr4 gene in crossbred cattle of kerala |
| topic | tlr4 pcr-sscp somatic cell score crossbred cattle |
| url | https://jvas.in/public_html/upload/article_file/article_file_rsdity.pdf?t=rsdity |
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