A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages
Herein, we report on a new type of ethanol biosensor based on a screen-printed electrode modified with poly(allylamine hydrochloride). The alcohol dehydrogenase was immobilized on the surface of the sensor using the sol–gel matrix. Working parameters such as applied potential, pH, NAD<sup>+<...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/21/19/6510 |
| _version_ | 1797515717401116672 |
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| author | Oana Maria Istrate Lucian Rotariu Camelia Bala |
| author_facet | Oana Maria Istrate Lucian Rotariu Camelia Bala |
| author_sort | Oana Maria Istrate |
| collection | DOAJ |
| description | Herein, we report on a new type of ethanol biosensor based on a screen-printed electrode modified with poly(allylamine hydrochloride). The alcohol dehydrogenase was immobilized on the surface of the sensor using the sol–gel matrix. Working parameters such as applied potential, pH, NAD<sup>+</sup> concentration, storage conditions were optimized. A response range between 0.05 and 2 mM was found with a sensitivity of 13.45 ± 0.67 µA/mM·cm<sup>2</sup> and a detection limit of 20 µM. The developed biosensor was used to detect ethanol in commercial beverages with good accuracy. |
| first_indexed | 2024-03-10T06:52:10Z |
| format | Article |
| id | doaj.art-7a8d26cb69e04158acf4cfc9b463af3a |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T06:52:10Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-7a8d26cb69e04158acf4cfc9b463af3a2023-11-22T16:47:11ZengMDPI AGSensors1424-82202021-09-012119651010.3390/s21196510A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in BeveragesOana Maria Istrate0Lucian Rotariu1Camelia Bala2LaborQ, University of Bucharest, 030018 Bucharest, RomaniaLaborQ, University of Bucharest, 030018 Bucharest, RomaniaLaborQ, University of Bucharest, 030018 Bucharest, RomaniaHerein, we report on a new type of ethanol biosensor based on a screen-printed electrode modified with poly(allylamine hydrochloride). The alcohol dehydrogenase was immobilized on the surface of the sensor using the sol–gel matrix. Working parameters such as applied potential, pH, NAD<sup>+</sup> concentration, storage conditions were optimized. A response range between 0.05 and 2 mM was found with a sensitivity of 13.45 ± 0.67 µA/mM·cm<sup>2</sup> and a detection limit of 20 µM. The developed biosensor was used to detect ethanol in commercial beverages with good accuracy.https://www.mdpi.com/1424-8220/21/19/6510alcohol dehydrogenasebiosensorscreen-printed electrodeethanol |
| spellingShingle | Oana Maria Istrate Lucian Rotariu Camelia Bala A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages Sensors alcohol dehydrogenase biosensor screen-printed electrode ethanol |
| title | A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages |
| title_full | A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages |
| title_fullStr | A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages |
| title_full_unstemmed | A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages |
| title_short | A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages |
| title_sort | novel amperometric biosensor based on poly allylamine hydrochloride for determination of ethanol in beverages |
| topic | alcohol dehydrogenase biosensor screen-printed electrode ethanol |
| url | https://www.mdpi.com/1424-8220/21/19/6510 |
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