Identification of Campylobacter jejuni and Campylobacter coli genes contributing to oxidative stress response using TraDIS analysis
Abstract Background Campylobacter jejuni and Campylobacter coli are the major causative agents of bacterial gastroenteritis worldwide and are known obligate microaerophiles. Despite being sensitive to oxygen and its reduction products, both species are readily isolated from animal food products kept...
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| Format: | Article |
| Language: | English |
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BMC
2024-02-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-024-03201-y |
| _version_ | 1797275688728788992 |
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| author | Emily Stoakes Xuanlin Chen Lajos Kalmar Dave Baker Rhiannon Evans Steven Rudder Andrew J. Grant |
| author_facet | Emily Stoakes Xuanlin Chen Lajos Kalmar Dave Baker Rhiannon Evans Steven Rudder Andrew J. Grant |
| author_sort | Emily Stoakes |
| collection | DOAJ |
| description | Abstract Background Campylobacter jejuni and Campylobacter coli are the major causative agents of bacterial gastroenteritis worldwide and are known obligate microaerophiles. Despite being sensitive to oxygen and its reduction products, both species are readily isolated from animal food products kept under atmospheric conditions where they face high oxygen tension levels. Results In this study, Transposon Directed Insertion-site Sequencing (TraDIS) was used to investigate the ability of one C. jejuni strain and two C. coli strains to overcome oxidative stress, using H2O2 to mimic oxidative stress. Genes were identified that were required for oxidative stress resistance for each individual strain but also allowed a comparison across the three strains. Mutations in the perR and ahpC genes were found to increase Campylobacter tolerance to H2O2. The roles of these proteins in oxidative stress were previously known in C. jejuni, but this data indicates that they most likely play a similar role in C. coli. Mutation of czcD decreased Campylobacter tolerance to H2O2. The role of CzcD, which functions as a zinc exporter, has not previously been linked to oxidative stress. The TraDIS data was confirmed using defined deletions of perR and czcD in C. coli 15-537360. Conclusions This is the first study to investigate gene fitness in both C. jejuni and C. coli under oxidative stress conditions and highlights both similar roles for certain genes for both species and highlights other genes that have a role under oxidative stress. |
| first_indexed | 2024-03-07T15:17:05Z |
| format | Article |
| id | doaj.art-7a8da63c09b7402fa7b18d34c11463a3 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-07T15:17:05Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-7a8da63c09b7402fa7b18d34c11463a32024-03-05T17:51:36ZengBMCBMC Microbiology1471-21802024-02-0124111110.1186/s12866-024-03201-yIdentification of Campylobacter jejuni and Campylobacter coli genes contributing to oxidative stress response using TraDIS analysisEmily Stoakes0Xuanlin Chen1Lajos Kalmar2Dave Baker3Rhiannon Evans4Steven Rudder5Andrew J. Grant6Department of Veterinary Medicine, University of CambridgeDepartment of Veterinary Medicine, University of CambridgeMRC Toxicology Unit, University of CambridgeQuadram Institute BioscienceQuadram Institute BioscienceQuadram Institute BioscienceDepartment of Veterinary Medicine, University of CambridgeAbstract Background Campylobacter jejuni and Campylobacter coli are the major causative agents of bacterial gastroenteritis worldwide and are known obligate microaerophiles. Despite being sensitive to oxygen and its reduction products, both species are readily isolated from animal food products kept under atmospheric conditions where they face high oxygen tension levels. Results In this study, Transposon Directed Insertion-site Sequencing (TraDIS) was used to investigate the ability of one C. jejuni strain and two C. coli strains to overcome oxidative stress, using H2O2 to mimic oxidative stress. Genes were identified that were required for oxidative stress resistance for each individual strain but also allowed a comparison across the three strains. Mutations in the perR and ahpC genes were found to increase Campylobacter tolerance to H2O2. The roles of these proteins in oxidative stress were previously known in C. jejuni, but this data indicates that they most likely play a similar role in C. coli. Mutation of czcD decreased Campylobacter tolerance to H2O2. The role of CzcD, which functions as a zinc exporter, has not previously been linked to oxidative stress. The TraDIS data was confirmed using defined deletions of perR and czcD in C. coli 15-537360. Conclusions This is the first study to investigate gene fitness in both C. jejuni and C. coli under oxidative stress conditions and highlights both similar roles for certain genes for both species and highlights other genes that have a role under oxidative stress.https://doi.org/10.1186/s12866-024-03201-yCampylobacter jejuniCampylobacter coliTraDISOxidative stressAerobic stress |
| spellingShingle | Emily Stoakes Xuanlin Chen Lajos Kalmar Dave Baker Rhiannon Evans Steven Rudder Andrew J. Grant Identification of Campylobacter jejuni and Campylobacter coli genes contributing to oxidative stress response using TraDIS analysis BMC Microbiology Campylobacter jejuni Campylobacter coli TraDIS Oxidative stress Aerobic stress |
| title | Identification of Campylobacter jejuni and Campylobacter coli genes contributing to oxidative stress response using TraDIS analysis |
| title_full | Identification of Campylobacter jejuni and Campylobacter coli genes contributing to oxidative stress response using TraDIS analysis |
| title_fullStr | Identification of Campylobacter jejuni and Campylobacter coli genes contributing to oxidative stress response using TraDIS analysis |
| title_full_unstemmed | Identification of Campylobacter jejuni and Campylobacter coli genes contributing to oxidative stress response using TraDIS analysis |
| title_short | Identification of Campylobacter jejuni and Campylobacter coli genes contributing to oxidative stress response using TraDIS analysis |
| title_sort | identification of campylobacter jejuni and campylobacter coli genes contributing to oxidative stress response using tradis analysis |
| topic | Campylobacter jejuni Campylobacter coli TraDIS Oxidative stress Aerobic stress |
| url | https://doi.org/10.1186/s12866-024-03201-y |
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