A Microfluidic Bioreactor with in Situ SERS Imaging for the Study of Controlled Flow Patterns of Biofilm Precursor Materials
A microfluidic bioreactor with an easy to fabricate nano-plasmonic surface is demonstrated for studies of biofilms and their precursor materials via Surface Enhanced Raman Spectroscopy (SERS). The system uses a novel design to induce sheath flow confinement of a sodium citrate biofilm precursor stre...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-10-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/13/11/14714 |
| _version_ | 1811184910675738624 |
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| author | Jesse Greener Muhammad Safdar Nahid Babaei Aznaveh François Paquet-Mercier |
| author_facet | Jesse Greener Muhammad Safdar Nahid Babaei Aznaveh François Paquet-Mercier |
| author_sort | Jesse Greener |
| collection | DOAJ |
| description | A microfluidic bioreactor with an easy to fabricate nano-plasmonic surface is demonstrated for studies of biofilms and their precursor materials via Surface Enhanced Raman Spectroscopy (SERS). The system uses a novel design to induce sheath flow confinement of a sodium citrate biofilm precursor stream against the SERS imaging surface to measure spatial variations in the concentration profile. The unoptimised SERS enhancement was approximately 2.5 × 104, thereby improving data acquisition time, reducing laser power requirements and enabling a citrate detection limit of 0.1 mM, which was well below the concentrations used in biofilm nutrient solutions. The flow confinement was observed by both optical microscopy and SERS imaging with good complementarity. We demonstrate the new bioreactor by growing flow-templated biofilms on the microchannel wall. This work opens the way for in situ spectral imaging of biofilms and their biochemical environment under dynamic flow conditions. |
| first_indexed | 2024-04-11T13:20:13Z |
| format | Article |
| id | doaj.art-7a8eab7b70074c3f9bec355cfb649f06 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T13:20:13Z |
| publishDate | 2013-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-7a8eab7b70074c3f9bec355cfb649f062022-12-22T04:22:12ZengMDPI AGSensors1424-82202013-10-011311147141472710.3390/s131114714A Microfluidic Bioreactor with in Situ SERS Imaging for the Study of Controlled Flow Patterns of Biofilm Precursor MaterialsJesse GreenerMuhammad SafdarNahid Babaei AznavehFrançois Paquet-MercierA microfluidic bioreactor with an easy to fabricate nano-plasmonic surface is demonstrated for studies of biofilms and their precursor materials via Surface Enhanced Raman Spectroscopy (SERS). The system uses a novel design to induce sheath flow confinement of a sodium citrate biofilm precursor stream against the SERS imaging surface to measure spatial variations in the concentration profile. The unoptimised SERS enhancement was approximately 2.5 × 104, thereby improving data acquisition time, reducing laser power requirements and enabling a citrate detection limit of 0.1 mM, which was well below the concentrations used in biofilm nutrient solutions. The flow confinement was observed by both optical microscopy and SERS imaging with good complementarity. We demonstrate the new bioreactor by growing flow-templated biofilms on the microchannel wall. This work opens the way for in situ spectral imaging of biofilms and their biochemical environment under dynamic flow conditions.http://www.mdpi.com/1424-8220/13/11/14714Surface Enhanced Raman Spectroscopyspectral imagingmicrofluidicbiofilmelectroless depositionbioreactorplasmonic surfaces |
| spellingShingle | Jesse Greener Muhammad Safdar Nahid Babaei Aznaveh François Paquet-Mercier A Microfluidic Bioreactor with in Situ SERS Imaging for the Study of Controlled Flow Patterns of Biofilm Precursor Materials Sensors Surface Enhanced Raman Spectroscopy spectral imaging microfluidic biofilm electroless deposition bioreactor plasmonic surfaces |
| title | A Microfluidic Bioreactor with in Situ SERS Imaging for the Study of Controlled Flow Patterns of Biofilm Precursor Materials |
| title_full | A Microfluidic Bioreactor with in Situ SERS Imaging for the Study of Controlled Flow Patterns of Biofilm Precursor Materials |
| title_fullStr | A Microfluidic Bioreactor with in Situ SERS Imaging for the Study of Controlled Flow Patterns of Biofilm Precursor Materials |
| title_full_unstemmed | A Microfluidic Bioreactor with in Situ SERS Imaging for the Study of Controlled Flow Patterns of Biofilm Precursor Materials |
| title_short | A Microfluidic Bioreactor with in Situ SERS Imaging for the Study of Controlled Flow Patterns of Biofilm Precursor Materials |
| title_sort | microfluidic bioreactor with in situ sers imaging for the study of controlled flow patterns of biofilm precursor materials |
| topic | Surface Enhanced Raman Spectroscopy spectral imaging microfluidic biofilm electroless deposition bioreactor plasmonic surfaces |
| url | http://www.mdpi.com/1424-8220/13/11/14714 |
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