Rating sputum cell quality in clinical trials for asthma and COPD treatment
Frauke Pedersen,1,2 Ulrich M Zissler,3 Henrik Watz,2 Klaus F Rabe,1 Jens M Hohlfeld,4,5 Olaf Holz41LungenClinic Grosshansdorf, Airway Research Center North (ARCN), German Center for Lung Research (DZL), Grosshansdorf, Germany; 2Pulmonary Research Institute at LungenClinic Grosshansdorf, Airway Resea...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2019-01-01
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| Series: | International Journal of COPD |
| Subjects: | |
| Online Access: | https://www.dovepress.com/rating-sputum-cell-quality-in-clinical-trials-for-asthma-and-copd-trea-peer-reviewed-article-COPD |
| _version_ | 1830478547508002816 |
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| author | Pedersen F Zissler UM Watz H Rabe KF Hohlfeld JM Holz O |
| author_facet | Pedersen F Zissler UM Watz H Rabe KF Hohlfeld JM Holz O |
| author_sort | Pedersen F |
| collection | DOAJ |
| description | Frauke Pedersen,1,2 Ulrich M Zissler,3 Henrik Watz,2 Klaus F Rabe,1 Jens M Hohlfeld,4,5 Olaf Holz41LungenClinic Grosshansdorf, Airway Research Center North (ARCN), German Center for Lung Research (DZL), Grosshansdorf, Germany; 2Pulmonary Research Institute at LungenClinic Grosshansdorf, Airway Research Center North (ARCN), German Center for Lung Research (DZL), Grosshansdorf, Germany; 3Center of Allergy & Environment (ZAUM), Technical University of Munich and Helmholtz Center Munich, German Research Center for Environmental Health, Germany, Member of the German Center for Lung Research (DZL), Munich, Germany; 4Fraunhofer ITEM, Clinical Airway Research – Biomedical Research in End-stage and Obstructive Lung Disease Hannover (BREATH), German Center for Lung Research (DZL), Hannover, Germany; 5Department of Respiratory Medicine, Hannover Medical School (MHH), Biomedical Research in End-stage and Obstructive Lung Disease Hannover (BREATH), Hannover, GermanyBackgroundInduced sputum is a method to assess airway inflammation in clinical trials for asthma and COPD.1–3 Sputum is a heterogeneous, viscous material containing inflammatory cell plugs, cellular debris, mucus, and saliva with squamous cells.The quality of sputum cell preparations for differential cell count analysis depends on multiple factors and can be highly variable.4,5 Percentage of squamous cell contamination (SQ%) is often used to assess the quality of sputum cell preparations.6Here, we evaluated a comprehensive quality score,7 which also includes an assessment of the inflammatory cell morphology and amount of cellular debris. |
| first_indexed | 2024-12-21T16:31:01Z |
| format | Article |
| id | doaj.art-7a8f313b7c5f417eb258b54fb681ecdb |
| institution | Directory Open Access Journal |
| issn | 1178-2005 |
| language | English |
| last_indexed | 2024-12-21T16:31:01Z |
| publishDate | 2019-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of COPD |
| spelling | doaj.art-7a8f313b7c5f417eb258b54fb681ecdb2022-12-21T18:57:21ZengDove Medical PressInternational Journal of COPD1178-20052019-01-01Volume 1419519843446Rating sputum cell quality in clinical trials for asthma and COPD treatmentPedersen FZissler UMWatz HRabe KFHohlfeld JMHolz OFrauke Pedersen,1,2 Ulrich M Zissler,3 Henrik Watz,2 Klaus F Rabe,1 Jens M Hohlfeld,4,5 Olaf Holz41LungenClinic Grosshansdorf, Airway Research Center North (ARCN), German Center for Lung Research (DZL), Grosshansdorf, Germany; 2Pulmonary Research Institute at LungenClinic Grosshansdorf, Airway Research Center North (ARCN), German Center for Lung Research (DZL), Grosshansdorf, Germany; 3Center of Allergy & Environment (ZAUM), Technical University of Munich and Helmholtz Center Munich, German Research Center for Environmental Health, Germany, Member of the German Center for Lung Research (DZL), Munich, Germany; 4Fraunhofer ITEM, Clinical Airway Research – Biomedical Research in End-stage and Obstructive Lung Disease Hannover (BREATH), German Center for Lung Research (DZL), Hannover, Germany; 5Department of Respiratory Medicine, Hannover Medical School (MHH), Biomedical Research in End-stage and Obstructive Lung Disease Hannover (BREATH), Hannover, GermanyBackgroundInduced sputum is a method to assess airway inflammation in clinical trials for asthma and COPD.1–3 Sputum is a heterogeneous, viscous material containing inflammatory cell plugs, cellular debris, mucus, and saliva with squamous cells.The quality of sputum cell preparations for differential cell count analysis depends on multiple factors and can be highly variable.4,5 Percentage of squamous cell contamination (SQ%) is often used to assess the quality of sputum cell preparations.6Here, we evaluated a comprehensive quality score,7 which also includes an assessment of the inflammatory cell morphology and amount of cellular debris. https://www.dovepress.com/rating-sputum-cell-quality-in-clinical-trials-for-asthma-and-copd-trea-peer-reviewed-article-COPDSputum cell qualityquality scoreclinical trialsairway inflammation |
| spellingShingle | Pedersen F Zissler UM Watz H Rabe KF Hohlfeld JM Holz O Rating sputum cell quality in clinical trials for asthma and COPD treatment International Journal of COPD Sputum cell quality quality score clinical trials airway inflammation |
| title | Rating sputum cell quality in clinical trials for asthma and COPD treatment |
| title_full | Rating sputum cell quality in clinical trials for asthma and COPD treatment |
| title_fullStr | Rating sputum cell quality in clinical trials for asthma and COPD treatment |
| title_full_unstemmed | Rating sputum cell quality in clinical trials for asthma and COPD treatment |
| title_short | Rating sputum cell quality in clinical trials for asthma and COPD treatment |
| title_sort | rating sputum cell quality in clinical trials for asthma and copd treatment |
| topic | Sputum cell quality quality score clinical trials airway inflammation |
| url | https://www.dovepress.com/rating-sputum-cell-quality-in-clinical-trials-for-asthma-and-copd-trea-peer-reviewed-article-COPD |
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