Chronic Stress Induces Type 2b Skeletal Muscle Atrophy via the Inhibition of mTORC1 Signaling in Mice
Chronic stress induces psychological and physiological changes that may have negative sequelae for health and well-being. In this study, the skeletal muscles of male C57BL/6 mice subjected to repetitive water-immersion restraint stress to model chronic stress were examined. In chronically stressed m...
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MDPI AG
2023-02-01
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| Series: | Medical Sciences |
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| Online Access: | https://www.mdpi.com/2076-3271/11/1/19 |
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| author | Shigeko Fushimi Tsutomu Nohno Hironobu Katsuyama |
| author_facet | Shigeko Fushimi Tsutomu Nohno Hironobu Katsuyama |
| author_sort | Shigeko Fushimi |
| collection | DOAJ |
| description | Chronic stress induces psychological and physiological changes that may have negative sequelae for health and well-being. In this study, the skeletal muscles of male C57BL/6 mice subjected to repetitive water-immersion restraint stress to model chronic stress were examined. In chronically stressed mice, serum corticosterone levels significantly increased, whereas thymus volume and bone mineral density decreased. Further, body weight, skeletal muscle mass, and grip strength were significantly decreased. Histochemical analysis of the soleus muscles revealed a significant decrease in the cross-sectional area of type 2b muscle fibers. Although type 2a fibers also tended to decrease, chronic stress had no impact on type 1 muscle fibers. Chronic stress increased the expression of <i>REDD1, FoxO1, FoxO3, KLF15, Atrogin1,</i> and <i>FKBP5</i>, but did not affect the expression of <i>myostatin</i> or <i>myogenin</i>. In contrast, chronic stress resulted in a decrease in p-S6 and p-4E-BP1 levels in the soleus muscle. Taken together, these results indicate that chronic stress promotes muscle atrophy by inhibiting mammalian targets of rapamycin complex 1 activity due to the upregulation of its inhibitor, <i>REDD1</i>. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-3271 |
| language | English |
| last_indexed | 2024-03-11T06:12:22Z |
| publishDate | 2023-02-01 |
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| spelling | doaj.art-7a90074d454c48d9becb5d056d9b59eb2023-11-17T12:33:52ZengMDPI AGMedical Sciences2076-32712023-02-011111910.3390/medsci11010019Chronic Stress Induces Type 2b Skeletal Muscle Atrophy via the Inhibition of mTORC1 Signaling in MiceShigeko Fushimi0Tsutomu Nohno1Hironobu Katsuyama2Department of Public Health, Kawasaki Medical School, 577 Matsushima, Kurashiki 701-0192, JapanDepartment of Public Health, Kawasaki Medical School, 577 Matsushima, Kurashiki 701-0192, JapanDepartment of Public Health, Kawasaki Medical School, 577 Matsushima, Kurashiki 701-0192, JapanChronic stress induces psychological and physiological changes that may have negative sequelae for health and well-being. In this study, the skeletal muscles of male C57BL/6 mice subjected to repetitive water-immersion restraint stress to model chronic stress were examined. In chronically stressed mice, serum corticosterone levels significantly increased, whereas thymus volume and bone mineral density decreased. Further, body weight, skeletal muscle mass, and grip strength were significantly decreased. Histochemical analysis of the soleus muscles revealed a significant decrease in the cross-sectional area of type 2b muscle fibers. Although type 2a fibers also tended to decrease, chronic stress had no impact on type 1 muscle fibers. Chronic stress increased the expression of <i>REDD1, FoxO1, FoxO3, KLF15, Atrogin1,</i> and <i>FKBP5</i>, but did not affect the expression of <i>myostatin</i> or <i>myogenin</i>. In contrast, chronic stress resulted in a decrease in p-S6 and p-4E-BP1 levels in the soleus muscle. Taken together, these results indicate that chronic stress promotes muscle atrophy by inhibiting mammalian targets of rapamycin complex 1 activity due to the upregulation of its inhibitor, <i>REDD1</i>.https://www.mdpi.com/2076-3271/11/1/19chronic stresssoleus muscletype 2b muscle fiberREDD1 inhibitionmTORC1 signaling |
| spellingShingle | Shigeko Fushimi Tsutomu Nohno Hironobu Katsuyama Chronic Stress Induces Type 2b Skeletal Muscle Atrophy via the Inhibition of mTORC1 Signaling in Mice Medical Sciences chronic stress soleus muscle type 2b muscle fiber REDD1 inhibition mTORC1 signaling |
| title | Chronic Stress Induces Type 2b Skeletal Muscle Atrophy via the Inhibition of mTORC1 Signaling in Mice |
| title_full | Chronic Stress Induces Type 2b Skeletal Muscle Atrophy via the Inhibition of mTORC1 Signaling in Mice |
| title_fullStr | Chronic Stress Induces Type 2b Skeletal Muscle Atrophy via the Inhibition of mTORC1 Signaling in Mice |
| title_full_unstemmed | Chronic Stress Induces Type 2b Skeletal Muscle Atrophy via the Inhibition of mTORC1 Signaling in Mice |
| title_short | Chronic Stress Induces Type 2b Skeletal Muscle Atrophy via the Inhibition of mTORC1 Signaling in Mice |
| title_sort | chronic stress induces type 2b skeletal muscle atrophy via the inhibition of mtorc1 signaling in mice |
| topic | chronic stress soleus muscle type 2b muscle fiber REDD1 inhibition mTORC1 signaling |
| url | https://www.mdpi.com/2076-3271/11/1/19 |
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