Evaluation of Cancer Cell Lines by Four-Point Probe Technique, by Impedance Measurements in Various Frequencies
Cell-based biosensors appear to be an attractive tool for the rapid, simple, and cheap monitoring of chemotherapy effects at a very early stage. In this study, electrochemical measurements using a four-point probe method were evaluated for suspensions of four cancer cell lines of different tissue or...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/9/345 |
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| author | Georgia Paivana Dimitris Barmpakos Sophie Mavrikou Alexandros Kallergis Odysseus Tsakiridis Grigoris Kaltsas Spyridon Kintzios |
| author_facet | Georgia Paivana Dimitris Barmpakos Sophie Mavrikou Alexandros Kallergis Odysseus Tsakiridis Grigoris Kaltsas Spyridon Kintzios |
| author_sort | Georgia Paivana |
| collection | DOAJ |
| description | Cell-based biosensors appear to be an attractive tool for the rapid, simple, and cheap monitoring of chemotherapy effects at a very early stage. In this study, electrochemical measurements using a four-point probe method were evaluated for suspensions of four cancer cell lines of different tissue origins: SK–N–SH, HeLa, MCF-7 and MDA-MB-231, all for two different population densities: 50 K and 100 K cells/500 μL. The anticancer agent doxorubicin was applied for each cell type in order to investigate whether the proposed technique was able to determine specific differences in cell responses before and after drug treatment. The proposed methodology can offer valuable insight into the frequency-dependent bioelectrical responses of various cellular systems using a low frequency range and without necessitating lengthy cell culture treatment. The further development of this biosensor assembly with the integration of specially designed cell/electronic interfaces can lead to novel diagnostic biosensors and therapeutic bioelectronics. |
| first_indexed | 2024-03-10T07:51:20Z |
| format | Article |
| id | doaj.art-7a91c78a67d94561824124fc968bccb2 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T07:51:20Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-7a91c78a67d94561824124fc968bccb22023-11-22T12:12:59ZengMDPI AGBiosensors2079-63742021-09-0111934510.3390/bios11090345Evaluation of Cancer Cell Lines by Four-Point Probe Technique, by Impedance Measurements in Various FrequenciesGeorgia Paivana0Dimitris Barmpakos1Sophie Mavrikou2Alexandros Kallergis3Odysseus Tsakiridis4Grigoris Kaltsas5Spyridon Kintzios6Laboratory of Cell Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 11855 Athens, GreecemicroSENSES Laboratory, Department of Electrical and Electronics Engineering, Faculty of Engineering, University of West Attica, 12244 Athens, GreeceLaboratory of Cell Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 11855 Athens, GreecemicroSENSES Laboratory, Department of Electrical and Electronics Engineering, Faculty of Engineering, University of West Attica, 12244 Athens, GreecemicroSENSES Laboratory, Department of Electrical and Electronics Engineering, Faculty of Engineering, University of West Attica, 12244 Athens, GreecemicroSENSES Laboratory, Department of Electrical and Electronics Engineering, Faculty of Engineering, University of West Attica, 12244 Athens, GreeceLaboratory of Cell Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 11855 Athens, GreeceCell-based biosensors appear to be an attractive tool for the rapid, simple, and cheap monitoring of chemotherapy effects at a very early stage. In this study, electrochemical measurements using a four-point probe method were evaluated for suspensions of four cancer cell lines of different tissue origins: SK–N–SH, HeLa, MCF-7 and MDA-MB-231, all for two different population densities: 50 K and 100 K cells/500 μL. The anticancer agent doxorubicin was applied for each cell type in order to investigate whether the proposed technique was able to determine specific differences in cell responses before and after drug treatment. The proposed methodology can offer valuable insight into the frequency-dependent bioelectrical responses of various cellular systems using a low frequency range and without necessitating lengthy cell culture treatment. The further development of this biosensor assembly with the integration of specially designed cell/electronic interfaces can lead to novel diagnostic biosensors and therapeutic bioelectronics.https://www.mdpi.com/2079-6374/11/9/345cancer cell linescell-based biosensordoxorubicinfour-point probe measurementspolydimethylsiloxane |
| spellingShingle | Georgia Paivana Dimitris Barmpakos Sophie Mavrikou Alexandros Kallergis Odysseus Tsakiridis Grigoris Kaltsas Spyridon Kintzios Evaluation of Cancer Cell Lines by Four-Point Probe Technique, by Impedance Measurements in Various Frequencies Biosensors cancer cell lines cell-based biosensor doxorubicin four-point probe measurements polydimethylsiloxane |
| title | Evaluation of Cancer Cell Lines by Four-Point Probe Technique, by Impedance Measurements in Various Frequencies |
| title_full | Evaluation of Cancer Cell Lines by Four-Point Probe Technique, by Impedance Measurements in Various Frequencies |
| title_fullStr | Evaluation of Cancer Cell Lines by Four-Point Probe Technique, by Impedance Measurements in Various Frequencies |
| title_full_unstemmed | Evaluation of Cancer Cell Lines by Four-Point Probe Technique, by Impedance Measurements in Various Frequencies |
| title_short | Evaluation of Cancer Cell Lines by Four-Point Probe Technique, by Impedance Measurements in Various Frequencies |
| title_sort | evaluation of cancer cell lines by four point probe technique by impedance measurements in various frequencies |
| topic | cancer cell lines cell-based biosensor doxorubicin four-point probe measurements polydimethylsiloxane |
| url | https://www.mdpi.com/2079-6374/11/9/345 |
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