Morphophysiological responses of Billbergia zebrina Lindl. (Bromeliaceae) in function of types and concentrations of carbohydrates during conventional in vitro culture
When propagated in vitro, explants receive all the nutrients needed for their growth, including carbohydrates, from the culture medium. However, it is not well understood how the type and concentration of carbohydrates can affect the functioning of the photosynthetic apparatus (particularly photosy...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Floricultura e Plantas Ornamentais
2020-01-01
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| Series: | Ornamental Horticulture |
| Subjects: | |
| Online Access: | https://ornamentalhorticulture.emnuvens.com.br/rbho/article/view/2092 |
| _version_ | 1797797472707280896 |
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| author | Elizangela Rodrigues Santos João Paulo Rodrigues Martins Luiz Carlos de Almeida Rodrigues Andreia Barcelos Passos Lima Gontijo Antelmo Ralph Falqueto |
| author_facet | Elizangela Rodrigues Santos João Paulo Rodrigues Martins Luiz Carlos de Almeida Rodrigues Andreia Barcelos Passos Lima Gontijo Antelmo Ralph Falqueto |
| author_sort | Elizangela Rodrigues Santos |
| collection | DOAJ |
| description |
When propagated in vitro, explants receive all the nutrients needed for their growth, including carbohydrates, from the culture medium. However, it is not well understood how the type and concentration of carbohydrates can affect the functioning of the photosynthetic apparatus (particularly photosystem II) of these plants. The aim was to assess the morphophysiological responses of Billbergia zebrina plants in function of sources and concentrations of carbohydrates during in vitro culture. Side shoots of plants previously established in vitro were individualized and transferred to a culture medium containing fructose, glucose or sucrose in four concentrations (0, 15, 30 or 45 g L-1). After growth for 55 days, the chlorophyll a fluorescence transient, leaf anatomy and growth were analyzed. The concentration and type of carbohydrate employed during in vitro culture did not decrease the photosynthetic apparatus performance. However, concentrations above 30 g L-1 led to anatomical modifications, revealing some degree of stress suffered by the plants. When grown in concentrations of 15 and 30 g L-1, irrespective of the carbohydrate used, the plants presented greater stomatal density. The supplementation of the culture medium with monosaccharides caused alterations in the development of the xylem vessels, such as increased number and diameter, allowing adjustment to the microenvironmental conditions. The in vitro conditions influenced the photosynthetic and anatomical responses of plants. The concentration interval from 15 to 30 g L-1 sucrose had a better effect by not causing large changes in the performance of the photosynthetic apparatus and anatomy of plants.
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| first_indexed | 2024-03-13T03:48:53Z |
| format | Article |
| id | doaj.art-7a923197d8674d9a8cac61cc3ea4831d |
| institution | Directory Open Access Journal |
| issn | 2447-536X |
| language | English |
| last_indexed | 2024-03-13T03:48:53Z |
| publishDate | 2020-01-01 |
| publisher | Sociedade Brasileira de Floricultura e Plantas Ornamentais |
| record_format | Article |
| series | Ornamental Horticulture |
| spelling | doaj.art-7a923197d8674d9a8cac61cc3ea4831d2023-06-22T20:34:47ZengSociedade Brasileira de Floricultura e Plantas OrnamentaisOrnamental Horticulture2447-536X2020-01-0126110.1590/2447-536X.v26i1.20922074Morphophysiological responses of Billbergia zebrina Lindl. (Bromeliaceae) in function of types and concentrations of carbohydrates during conventional in vitro cultureElizangela Rodrigues Santos0João Paulo Rodrigues Martins1Luiz Carlos de Almeida Rodrigues2Andreia Barcelos Passos Lima Gontijo3Antelmo Ralph Falqueto4Federal University of Espírito Santo, Department of Agrarian and Biological Sciences, São Mateus-ESFederal University of Espírito Santo, Department of Agrarian and Biological Sciences, São Mateus-ESFederal University of Alfenas, Alfenas-MGFederal University of Espírito Santo, Department of Agrarian and Biological Sciences, São Mateus-ESFederal University of Espírito Santo, Department of Agrarian and Biological Sciences, São Mateus-ES When propagated in vitro, explants receive all the nutrients needed for their growth, including carbohydrates, from the culture medium. However, it is not well understood how the type and concentration of carbohydrates can affect the functioning of the photosynthetic apparatus (particularly photosystem II) of these plants. The aim was to assess the morphophysiological responses of Billbergia zebrina plants in function of sources and concentrations of carbohydrates during in vitro culture. Side shoots of plants previously established in vitro were individualized and transferred to a culture medium containing fructose, glucose or sucrose in four concentrations (0, 15, 30 or 45 g L-1). After growth for 55 days, the chlorophyll a fluorescence transient, leaf anatomy and growth were analyzed. The concentration and type of carbohydrate employed during in vitro culture did not decrease the photosynthetic apparatus performance. However, concentrations above 30 g L-1 led to anatomical modifications, revealing some degree of stress suffered by the plants. When grown in concentrations of 15 and 30 g L-1, irrespective of the carbohydrate used, the plants presented greater stomatal density. The supplementation of the culture medium with monosaccharides caused alterations in the development of the xylem vessels, such as increased number and diameter, allowing adjustment to the microenvironmental conditions. The in vitro conditions influenced the photosynthetic and anatomical responses of plants. The concentration interval from 15 to 30 g L-1 sucrose had a better effect by not causing large changes in the performance of the photosynthetic apparatus and anatomy of plants. https://ornamentalhorticulture.emnuvens.com.br/rbho/article/view/2092bromeliadchlorophyll a fluorescenceplant anatomyplant physiologyplant tissue culture. |
| spellingShingle | Elizangela Rodrigues Santos João Paulo Rodrigues Martins Luiz Carlos de Almeida Rodrigues Andreia Barcelos Passos Lima Gontijo Antelmo Ralph Falqueto Morphophysiological responses of Billbergia zebrina Lindl. (Bromeliaceae) in function of types and concentrations of carbohydrates during conventional in vitro culture Ornamental Horticulture bromeliad chlorophyll a fluorescence plant anatomy plant physiology plant tissue culture. |
| title | Morphophysiological responses of Billbergia zebrina Lindl. (Bromeliaceae) in function of types and concentrations of carbohydrates during conventional in vitro culture |
| title_full | Morphophysiological responses of Billbergia zebrina Lindl. (Bromeliaceae) in function of types and concentrations of carbohydrates during conventional in vitro culture |
| title_fullStr | Morphophysiological responses of Billbergia zebrina Lindl. (Bromeliaceae) in function of types and concentrations of carbohydrates during conventional in vitro culture |
| title_full_unstemmed | Morphophysiological responses of Billbergia zebrina Lindl. (Bromeliaceae) in function of types and concentrations of carbohydrates during conventional in vitro culture |
| title_short | Morphophysiological responses of Billbergia zebrina Lindl. (Bromeliaceae) in function of types and concentrations of carbohydrates during conventional in vitro culture |
| title_sort | morphophysiological responses of billbergia zebrina lindl bromeliaceae in function of types and concentrations of carbohydrates during conventional in vitro culture |
| topic | bromeliad chlorophyll a fluorescence plant anatomy plant physiology plant tissue culture. |
| url | https://ornamentalhorticulture.emnuvens.com.br/rbho/article/view/2092 |
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