Metabolomics Analysis of Health Functions of Physalis Pubescens L. using by Ultra-performance Liquid Chromatography/Electrospray Ionization Quadruple Time-of-Flight Mass Spectrometry
Herbal medicines may benefit from metabolomics studies, and applying metabolomics may provide answers about which herbal interventions may be effective for individuals, which metabolic processes are triggered, and the subsequent chemical pathways of activity. Physalis pubescens L (PPL) is an herbal...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-07-01
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| Series: | World Journal of Traditional Chinese Medicine |
| Subjects: | |
| Online Access: | http://www.wjtcm.org:8080/ch/reader/create_pdf.aspx?file_no=20150015&year_id=2015&quarter_id=1&flag=1 |
| _version_ | 1828404941344997376 |
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| author | Hang Chu Hui Sun Guang-Li Yan Ai-Hua Zhang Chang Liu Hui Dong Xiang-Cai Meng Xi-Jun Wang |
| author_facet | Hang Chu Hui Sun Guang-Li Yan Ai-Hua Zhang Chang Liu Hui Dong Xiang-Cai Meng Xi-Jun Wang |
| author_sort | Hang Chu |
| collection | DOAJ |
| description | Herbal medicines may benefit from metabolomics studies, and applying metabolomics may provide answers about which herbal interventions may be effective for individuals, which metabolic processes are triggered, and the subsequent chemical pathways of activity. Physalis pubescens L (PPL) is an herbal fruit for one year living plant and has been developed into healthy function's food. However, the mechanisms of health functions are still unclear. To comprehensively and holistically assess its anti-fatigue and antioxidant effects, a novel integrative metabolomics approach was applied. In this study, we present metabolomics analysis applying ultra performance liquid chromatography coupled to quadrupole with time-of-flight mass spectrometry (UPLC-Q/TOF-MS) to determine metabolite alterations after oral administration PPL to rats. Fifteen metabolites in urine were identified as potential biomarkers. Pattern analysis of the UPLC-Q/TOF-MS data disclosed that PPL could relieve fatigue rats by ameliorating the disturbance in amino acids metabolism and energy metabolism, alleviating the oxidative stress from reactive oxygen species and the inflammatory damage, and recovering the destructed regulation. Based on these results, we demonstrated that PPL is a promising source of natural anti-fatigue and antioxidants material for use in functional foods and medicines. |
| first_indexed | 2024-12-10T10:44:38Z |
| format | Article |
| id | doaj.art-7a923820d8ad42419e8e329886f51c75 |
| institution | Directory Open Access Journal |
| issn | 2311-8571 |
| language | English |
| last_indexed | 2024-12-10T10:44:38Z |
| publishDate | 2015-07-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | World Journal of Traditional Chinese Medicine |
| spelling | doaj.art-7a923820d8ad42419e8e329886f51c752022-12-22T01:52:12ZengWolters Kluwer Medknow PublicationsWorld Journal of Traditional Chinese Medicine2311-85712015-07-011392010.15806/j.issn.2311-8571.2015.00152015.0015Metabolomics Analysis of Health Functions of Physalis Pubescens L. using by Ultra-performance Liquid Chromatography/Electrospray Ionization Quadruple Time-of-Flight Mass SpectrometryHang ChuHui SunGuang-Li YanAi-Hua ZhangChang LiuHui DongXiang-Cai MengXi-Jun WangHerbal medicines may benefit from metabolomics studies, and applying metabolomics may provide answers about which herbal interventions may be effective for individuals, which metabolic processes are triggered, and the subsequent chemical pathways of activity. Physalis pubescens L (PPL) is an herbal fruit for one year living plant and has been developed into healthy function's food. However, the mechanisms of health functions are still unclear. To comprehensively and holistically assess its anti-fatigue and antioxidant effects, a novel integrative metabolomics approach was applied. In this study, we present metabolomics analysis applying ultra performance liquid chromatography coupled to quadrupole with time-of-flight mass spectrometry (UPLC-Q/TOF-MS) to determine metabolite alterations after oral administration PPL to rats. Fifteen metabolites in urine were identified as potential biomarkers. Pattern analysis of the UPLC-Q/TOF-MS data disclosed that PPL could relieve fatigue rats by ameliorating the disturbance in amino acids metabolism and energy metabolism, alleviating the oxidative stress from reactive oxygen species and the inflammatory damage, and recovering the destructed regulation. Based on these results, we demonstrated that PPL is a promising source of natural anti-fatigue and antioxidants material for use in functional foods and medicines.http://www.wjtcm.org:8080/ch/reader/create_pdf.aspx?file_no=20150015&year_id=2015&quarter_id=1&flag=1MetabolomicsPhysalis pubescens Lantioxidantanti-fatiguemetabolic pathwaybiomarkers |
| spellingShingle | Hang Chu Hui Sun Guang-Li Yan Ai-Hua Zhang Chang Liu Hui Dong Xiang-Cai Meng Xi-Jun Wang Metabolomics Analysis of Health Functions of Physalis Pubescens L. using by Ultra-performance Liquid Chromatography/Electrospray Ionization Quadruple Time-of-Flight Mass Spectrometry World Journal of Traditional Chinese Medicine Metabolomics Physalis pubescens L antioxidant anti-fatigue metabolic pathway biomarkers |
| title | Metabolomics Analysis of Health Functions of Physalis Pubescens L. using by Ultra-performance Liquid Chromatography/Electrospray Ionization Quadruple Time-of-Flight Mass Spectrometry |
| title_full | Metabolomics Analysis of Health Functions of Physalis Pubescens L. using by Ultra-performance Liquid Chromatography/Electrospray Ionization Quadruple Time-of-Flight Mass Spectrometry |
| title_fullStr | Metabolomics Analysis of Health Functions of Physalis Pubescens L. using by Ultra-performance Liquid Chromatography/Electrospray Ionization Quadruple Time-of-Flight Mass Spectrometry |
| title_full_unstemmed | Metabolomics Analysis of Health Functions of Physalis Pubescens L. using by Ultra-performance Liquid Chromatography/Electrospray Ionization Quadruple Time-of-Flight Mass Spectrometry |
| title_short | Metabolomics Analysis of Health Functions of Physalis Pubescens L. using by Ultra-performance Liquid Chromatography/Electrospray Ionization Quadruple Time-of-Flight Mass Spectrometry |
| title_sort | metabolomics analysis of health functions of physalis pubescens l using by ultra performance liquid chromatography electrospray ionization quadruple time of flight mass spectrometry |
| topic | Metabolomics Physalis pubescens L antioxidant anti-fatigue metabolic pathway biomarkers |
| url | http://www.wjtcm.org:8080/ch/reader/create_pdf.aspx?file_no=20150015&year_id=2015&quarter_id=1&flag=1 |
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