Characterization of Awp14, A Novel Cluster III Adhesin Identified in a High Biofilm-Forming Candida glabrata Isolate
Candida glabrata is among the most prevalent causes of candidiasis. Unlike Candida albicans, it is not capable of changing morphology between yeast and hyphal forms but instead has developed other virulence factors. An important feature is its unprecedented large repertoire of predicted cell wall ad...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-11-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.790465/full |
| _version_ | 1818892726541746176 |
|---|---|
| author | Jordan Fernández-Pereira María Alvarado Emilia Gómez-Molero Emilia Gómez-Molero Henk L. Dekker María Teresa Blázquez-Muñoz Elena Eraso Oliver Bader Piet W. J. de Groot |
| author_facet | Jordan Fernández-Pereira María Alvarado Emilia Gómez-Molero Emilia Gómez-Molero Henk L. Dekker María Teresa Blázquez-Muñoz Elena Eraso Oliver Bader Piet W. J. de Groot |
| author_sort | Jordan Fernández-Pereira |
| collection | DOAJ |
| description | Candida glabrata is among the most prevalent causes of candidiasis. Unlike Candida albicans, it is not capable of changing morphology between yeast and hyphal forms but instead has developed other virulence factors. An important feature is its unprecedented large repertoire of predicted cell wall adhesins, which are thought to enable adherence to a variety of surfaces under different conditions. Here, we analyzed the wall proteome of PEU1221, a high biofilm-forming clinical strain isolated from an infected central venous catheter, under biofilm-forming conditions. This isolate shows increased incorporation of putative adhesins, including eight proteins that were not detected in walls of reference strain ATCC 2001, and of which Epa22, Awp14, and Awp2e were identified for the first time. The proteomics data suggest that cluster III adhesin Awp14 is relatively abundant in PEU1221. Phenotypic studies with awp14Δ deletion mutants showed that Awp14 is not responsible for the high biofilm formation of PEU1221 onto polystyrene. However, awp14Δ mutant cells in PEU1221 background showed a slightly diminished binding to chitin and seemed to sediment slightly slower than the parental strain suggesting implication in fungal cell-cell interactions. By structural modeling, we further demonstrate similarity between the ligand-binding domains of cluster III adhesin Awp14 and those of cluster V and VI adhesins. In conclusion, our work confirms the increased incorporation of putative adhesins, such as Awp14, in high biofilm-forming isolates, and contributes to decipher the precise role of these proteins in the establishment of C. glabrata infections. |
| first_indexed | 2024-12-19T18:01:17Z |
| format | Article |
| id | doaj.art-7a928480f0e14e1abba0c1c1562af535 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-19T18:01:17Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-7a928480f0e14e1abba0c1c1562af5352022-12-21T20:11:40ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-11-011110.3389/fcimb.2021.790465790465Characterization of Awp14, A Novel Cluster III Adhesin Identified in a High Biofilm-Forming Candida glabrata IsolateJordan Fernández-Pereira0María Alvarado1Emilia Gómez-Molero2Emilia Gómez-Molero3Henk L. Dekker4María Teresa Blázquez-Muñoz5Elena Eraso6Oliver Bader7Piet W. J. de Groot8Albacete Regional Center for Biomedical Research, Castilla - La Mancha Science & Technology Park, University of Castilla-La Mancha, Albacete, SpainAlbacete Regional Center for Biomedical Research, Castilla - La Mancha Science & Technology Park, University of Castilla-La Mancha, Albacete, SpainAlbacete Regional Center for Biomedical Research, Castilla - La Mancha Science & Technology Park, University of Castilla-La Mancha, Albacete, SpainInstitute for Medical Microbiology, University Medical Center Göttingen, Göttingen, GermanyMass Spectrometry of Biomolecules, Swammerdam Institute for Life Sciences Amsterdam, University of Amsterdam, Amsterdam, NetherlandsAlbacete Regional Center for Biomedical Research, Castilla - La Mancha Science & Technology Park, University of Castilla-La Mancha, Albacete, SpainMass Spectrometry of Biomolecules, Swammerdam Institute for Life Sciences Amsterdam, University of Amsterdam, Amsterdam, NetherlandsInstitute for Medical Microbiology, University Medical Center Göttingen, Göttingen, GermanyAlbacete Regional Center for Biomedical Research, Castilla - La Mancha Science & Technology Park, University of Castilla-La Mancha, Albacete, SpainCandida glabrata is among the most prevalent causes of candidiasis. Unlike Candida albicans, it is not capable of changing morphology between yeast and hyphal forms but instead has developed other virulence factors. An important feature is its unprecedented large repertoire of predicted cell wall adhesins, which are thought to enable adherence to a variety of surfaces under different conditions. Here, we analyzed the wall proteome of PEU1221, a high biofilm-forming clinical strain isolated from an infected central venous catheter, under biofilm-forming conditions. This isolate shows increased incorporation of putative adhesins, including eight proteins that were not detected in walls of reference strain ATCC 2001, and of which Epa22, Awp14, and Awp2e were identified for the first time. The proteomics data suggest that cluster III adhesin Awp14 is relatively abundant in PEU1221. Phenotypic studies with awp14Δ deletion mutants showed that Awp14 is not responsible for the high biofilm formation of PEU1221 onto polystyrene. However, awp14Δ mutant cells in PEU1221 background showed a slightly diminished binding to chitin and seemed to sediment slightly slower than the parental strain suggesting implication in fungal cell-cell interactions. By structural modeling, we further demonstrate similarity between the ligand-binding domains of cluster III adhesin Awp14 and those of cluster V and VI adhesins. In conclusion, our work confirms the increased incorporation of putative adhesins, such as Awp14, in high biofilm-forming isolates, and contributes to decipher the precise role of these proteins in the establishment of C. glabrata infections.https://www.frontiersin.org/articles/10.3389/fcimb.2021.790465/fulladhesionGPI proteincell wall proteinhost-pathogen interactionsCandida glabratacandidiasis |
| spellingShingle | Jordan Fernández-Pereira María Alvarado Emilia Gómez-Molero Emilia Gómez-Molero Henk L. Dekker María Teresa Blázquez-Muñoz Elena Eraso Oliver Bader Piet W. J. de Groot Characterization of Awp14, A Novel Cluster III Adhesin Identified in a High Biofilm-Forming Candida glabrata Isolate Frontiers in Cellular and Infection Microbiology adhesion GPI protein cell wall protein host-pathogen interactions Candida glabrata candidiasis |
| title | Characterization of Awp14, A Novel Cluster III Adhesin Identified in a High Biofilm-Forming Candida glabrata Isolate |
| title_full | Characterization of Awp14, A Novel Cluster III Adhesin Identified in a High Biofilm-Forming Candida glabrata Isolate |
| title_fullStr | Characterization of Awp14, A Novel Cluster III Adhesin Identified in a High Biofilm-Forming Candida glabrata Isolate |
| title_full_unstemmed | Characterization of Awp14, A Novel Cluster III Adhesin Identified in a High Biofilm-Forming Candida glabrata Isolate |
| title_short | Characterization of Awp14, A Novel Cluster III Adhesin Identified in a High Biofilm-Forming Candida glabrata Isolate |
| title_sort | characterization of awp14 a novel cluster iii adhesin identified in a high biofilm forming candida glabrata isolate |
| topic | adhesion GPI protein cell wall protein host-pathogen interactions Candida glabrata candidiasis |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.790465/full |
| work_keys_str_mv | AT jordanfernandezpereira characterizationofawp14anovelclusteriiiadhesinidentifiedinahighbiofilmformingcandidaglabrataisolate AT mariaalvarado characterizationofawp14anovelclusteriiiadhesinidentifiedinahighbiofilmformingcandidaglabrataisolate AT emiliagomezmolero characterizationofawp14anovelclusteriiiadhesinidentifiedinahighbiofilmformingcandidaglabrataisolate AT emiliagomezmolero characterizationofawp14anovelclusteriiiadhesinidentifiedinahighbiofilmformingcandidaglabrataisolate AT henkldekker characterizationofawp14anovelclusteriiiadhesinidentifiedinahighbiofilmformingcandidaglabrataisolate AT mariateresablazquezmunoz characterizationofawp14anovelclusteriiiadhesinidentifiedinahighbiofilmformingcandidaglabrataisolate AT elenaeraso characterizationofawp14anovelclusteriiiadhesinidentifiedinahighbiofilmformingcandidaglabrataisolate AT oliverbader characterizationofawp14anovelclusteriiiadhesinidentifiedinahighbiofilmformingcandidaglabrataisolate AT pietwjdegroot characterizationofawp14anovelclusteriiiadhesinidentifiedinahighbiofilmformingcandidaglabrataisolate |