SKRINING DAN IDENTIFIKASI ISOLAT BAKTERI ENDOFIT UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI PADA BAWANG MERAH
Screening and identification of endophytic bacteria to control bacterial leaf blight disease on Shallot. The experiment was conducted in Laboratory and Green House, from January to June 2012. Laboratory experiment consisted of three steps: (1) isolation of endophytic bacteria from healthy onion r...
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| Format: | Article |
| Language: | English |
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Universitas Lampung
2014-08-01
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| Series: | Jurnal Hama dan Penyakit Tumbuhan Tropika |
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| Online Access: | http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/72 |
| _version_ | 1818111870090870784 |
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| author | Zurai Resti Trimurti Habazar Deddi Prima Putra . Nasrun |
| author_facet | Zurai Resti Trimurti Habazar Deddi Prima Putra . Nasrun |
| author_sort | Zurai Resti |
| collection | DOAJ |
| description | Screening and identification of endophytic bacteria to control bacterial leaf blight disease on Shallot. The experiment was conducted in Laboratory and Green House, from January to June 2012. Laboratory experiment consisted of three steps: (1) isolation of endophytic bacteria from healthy onion roots, (2)In planta /screening of endophytic isolates capable of reducing bacterial leaf blight disease, and (3) molecular identification of potential endophytic isolates. Treatments of in planta test were arranged in Completely Randomized Design. Collected isolates were tested for their capability in controlling bacterial leaf blight disease on shallot. The variables observed were disease incidence, disease severity, and shallot yield. The results showed that out of 82 isolates successfully isolated, 56 isolates (68.29%) were Gram positive, and 26 isolate (31.71%) were Gram negative. All isolates were HR negative and pathogenicity negative. Six endophytic isolates showed better performance in inducing resistance and increasing onion yield. Based on 16S rRNA sequence the six isolates were Bacillus cereus strain P14 , Bacillus cereus strain Se07, Bacillus sp H1, Bacillus sp SJ1 and Serratia marcescens strain PPM4. |
| first_indexed | 2024-12-11T03:09:54Z |
| format | Article |
| id | doaj.art-7a92e3307d974a8f842abdabc928dde0 |
| institution | Directory Open Access Journal |
| issn | 1411-7525 2461-0399 |
| language | English |
| last_indexed | 2024-12-11T03:09:54Z |
| publishDate | 2014-08-01 |
| publisher | Universitas Lampung |
| record_format | Article |
| series | Jurnal Hama dan Penyakit Tumbuhan Tropika |
| spelling | doaj.art-7a92e3307d974a8f842abdabc928dde02022-12-22T01:22:53ZengUniversitas LampungJurnal Hama dan Penyakit Tumbuhan Tropika1411-75252461-03992014-08-0113216717810.23960/j.hptt.213167-17856SKRINING DAN IDENTIFIKASI ISOLAT BAKTERI ENDOFIT UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI PADA BAWANG MERAHZurai Resti0Trimurti Habazar1Deddi Prima Putra2. Nasrun3Mahasiswa Pascasarjana Universitas Andalas, Padang, Sumatera Barat Jl. Alang Lawas IV No. 33 Padang 25211Dosen Fakultas Pertanian Universitas Andalas, Padang, Sumatera BaratDosen Fakultas Farmasi Universitas Andalas, Padang, Sumatera BaratPeneliti pada Balai Penelitian Tanaman Obat dan Aromatik, Laing Solok, Sumatera BaratScreening and identification of endophytic bacteria to control bacterial leaf blight disease on Shallot. The experiment was conducted in Laboratory and Green House, from January to June 2012. Laboratory experiment consisted of three steps: (1) isolation of endophytic bacteria from healthy onion roots, (2)In planta /screening of endophytic isolates capable of reducing bacterial leaf blight disease, and (3) molecular identification of potential endophytic isolates. Treatments of in planta test were arranged in Completely Randomized Design. Collected isolates were tested for their capability in controlling bacterial leaf blight disease on shallot. The variables observed were disease incidence, disease severity, and shallot yield. The results showed that out of 82 isolates successfully isolated, 56 isolates (68.29%) were Gram positive, and 26 isolate (31.71%) were Gram negative. All isolates were HR negative and pathogenicity negative. Six endophytic isolates showed better performance in inducing resistance and increasing onion yield. Based on 16S rRNA sequence the six isolates were Bacillus cereus strain P14 , Bacillus cereus strain Se07, Bacillus sp H1, Bacillus sp SJ1 and Serratia marcescens strain PPM4.http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/72bacterial leaf blightendophytic bacteriaXanthomonas axonopodis pv alliiincidenceseverity |
| spellingShingle | Zurai Resti Trimurti Habazar Deddi Prima Putra . Nasrun SKRINING DAN IDENTIFIKASI ISOLAT BAKTERI ENDOFIT UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI PADA BAWANG MERAH Jurnal Hama dan Penyakit Tumbuhan Tropika bacterial leaf blight endophytic bacteria Xanthomonas axonopodis pv allii incidence severity |
| title | SKRINING DAN IDENTIFIKASI ISOLAT BAKTERI ENDOFIT UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI PADA BAWANG MERAH |
| title_full | SKRINING DAN IDENTIFIKASI ISOLAT BAKTERI ENDOFIT UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI PADA BAWANG MERAH |
| title_fullStr | SKRINING DAN IDENTIFIKASI ISOLAT BAKTERI ENDOFIT UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI PADA BAWANG MERAH |
| title_full_unstemmed | SKRINING DAN IDENTIFIKASI ISOLAT BAKTERI ENDOFIT UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI PADA BAWANG MERAH |
| title_short | SKRINING DAN IDENTIFIKASI ISOLAT BAKTERI ENDOFIT UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI PADA BAWANG MERAH |
| title_sort | skrining dan identifikasi isolat bakteri endofit untuk mengendalikan penyakit hawar daun bakteri pada bawang merah |
| topic | bacterial leaf blight endophytic bacteria Xanthomonas axonopodis pv allii incidence severity |
| url | http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/72 |
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