Exopolysaccharide from <i>Lactiplantibacillus plantarum</i> YT013 and Its Apoptotic Activity on Gastric Cancer AGS Cells
Cancer is a significant health burden in the world. Natural product drugs have received widespread attention because of their safety and effectiveness, stable effects and fewer side effects. Some studies have demonstrated that exopolysaccharide (EPS) from lactic acid bacteria (LAB) can inhibit the g...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/6/539 |
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| author | Rentao Zhang Zhongkun Zhou Yunhao Ma Kangjia Du Mengze Sun Hao Zhang Hongyuan Tu Xinrong Jiang Juan Lu Lixue Tu Yuqing Niu Peng Chen |
| author_facet | Rentao Zhang Zhongkun Zhou Yunhao Ma Kangjia Du Mengze Sun Hao Zhang Hongyuan Tu Xinrong Jiang Juan Lu Lixue Tu Yuqing Niu Peng Chen |
| author_sort | Rentao Zhang |
| collection | DOAJ |
| description | Cancer is a significant health burden in the world. Natural product drugs have received widespread attention because of their safety and effectiveness, stable effects and fewer side effects. Some studies have demonstrated that exopolysaccharide (EPS) from lactic acid bacteria (LAB) can inhibit the growth of many types of cancer cells. In this work, the effects of the EPS from <i>Lactiplantibacillus plantarum</i> YT013 on gastric cancer cells were investigated. Its cytotoxicity was evaluated by MTT assay; at the concentration of 1000 μg/mL, the most significant inhibitory effect occurred in AGS cells, followed by SGC-7901, PANC-1 and HCT116, and less inhibited in HepG2 cells. Cell cycle results showed that EPS prevented AGS cells from transitioning from the S phase to the G<sub>2</sub>/M phase. In addition, the results of flow cytometry showed that EPS promoted apoptosis in a concentration-dependent manner. Western blotting also indicated that EPS might lead to apoptosis via the endogenous mitochondrial apoptotic pathway. The safety of lyophilized powder, cell-free culture supernatant and EPS from <i>Lactiplantibacillus plantarum</i> YT013 were evaluated by observing tissue organs through H&E staining, and the results showed that the components were safe and effective and could provide a basis for the development of natural anticancer active drugs. |
| first_indexed | 2024-03-11T02:29:46Z |
| format | Article |
| id | doaj.art-7a9413b2b0c04bea86fbbb788f2787ff |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T02:29:46Z |
| publishDate | 2023-05-01 |
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| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-7a9413b2b0c04bea86fbbb788f2787ff2023-11-18T10:21:09ZengMDPI AGFermentation2311-56372023-05-019653910.3390/fermentation9060539Exopolysaccharide from <i>Lactiplantibacillus plantarum</i> YT013 and Its Apoptotic Activity on Gastric Cancer AGS CellsRentao Zhang0Zhongkun Zhou1Yunhao Ma2Kangjia Du3Mengze Sun4Hao Zhang5Hongyuan Tu6Xinrong Jiang7Juan Lu8Lixue Tu9Yuqing Niu10Peng Chen11School of Pharmacy, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, ChinaSchool of Pharmacy, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, ChinaSchool of Pharmacy, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, ChinaSchool of Pharmacy, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, ChinaSchool of Pharmacy, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, ChinaSchool of Pharmacy, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, ChinaSchool of Pharmacy, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, ChinaSchool of Pharmacy, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, ChinaSchool of Pharmacy, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, ChinaSchool of Pharmacy, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, ChinaSchool of Pharmacy, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, ChinaSchool of Pharmacy, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, ChinaCancer is a significant health burden in the world. Natural product drugs have received widespread attention because of their safety and effectiveness, stable effects and fewer side effects. Some studies have demonstrated that exopolysaccharide (EPS) from lactic acid bacteria (LAB) can inhibit the growth of many types of cancer cells. In this work, the effects of the EPS from <i>Lactiplantibacillus plantarum</i> YT013 on gastric cancer cells were investigated. Its cytotoxicity was evaluated by MTT assay; at the concentration of 1000 μg/mL, the most significant inhibitory effect occurred in AGS cells, followed by SGC-7901, PANC-1 and HCT116, and less inhibited in HepG2 cells. Cell cycle results showed that EPS prevented AGS cells from transitioning from the S phase to the G<sub>2</sub>/M phase. In addition, the results of flow cytometry showed that EPS promoted apoptosis in a concentration-dependent manner. Western blotting also indicated that EPS might lead to apoptosis via the endogenous mitochondrial apoptotic pathway. The safety of lyophilized powder, cell-free culture supernatant and EPS from <i>Lactiplantibacillus plantarum</i> YT013 were evaluated by observing tissue organs through H&E staining, and the results showed that the components were safe and effective and could provide a basis for the development of natural anticancer active drugs.https://www.mdpi.com/2311-5637/9/6/539AGS cellsapoptosisexopolysaccharide<i>Lactiplantibacillus plantarum</i>mitochondrial membrane potential |
| spellingShingle | Rentao Zhang Zhongkun Zhou Yunhao Ma Kangjia Du Mengze Sun Hao Zhang Hongyuan Tu Xinrong Jiang Juan Lu Lixue Tu Yuqing Niu Peng Chen Exopolysaccharide from <i>Lactiplantibacillus plantarum</i> YT013 and Its Apoptotic Activity on Gastric Cancer AGS Cells Fermentation AGS cells apoptosis exopolysaccharide <i>Lactiplantibacillus plantarum</i> mitochondrial membrane potential |
| title | Exopolysaccharide from <i>Lactiplantibacillus plantarum</i> YT013 and Its Apoptotic Activity on Gastric Cancer AGS Cells |
| title_full | Exopolysaccharide from <i>Lactiplantibacillus plantarum</i> YT013 and Its Apoptotic Activity on Gastric Cancer AGS Cells |
| title_fullStr | Exopolysaccharide from <i>Lactiplantibacillus plantarum</i> YT013 and Its Apoptotic Activity on Gastric Cancer AGS Cells |
| title_full_unstemmed | Exopolysaccharide from <i>Lactiplantibacillus plantarum</i> YT013 and Its Apoptotic Activity on Gastric Cancer AGS Cells |
| title_short | Exopolysaccharide from <i>Lactiplantibacillus plantarum</i> YT013 and Its Apoptotic Activity on Gastric Cancer AGS Cells |
| title_sort | exopolysaccharide from i lactiplantibacillus plantarum i yt013 and its apoptotic activity on gastric cancer ags cells |
| topic | AGS cells apoptosis exopolysaccharide <i>Lactiplantibacillus plantarum</i> mitochondrial membrane potential |
| url | https://www.mdpi.com/2311-5637/9/6/539 |
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