Attenuated suppression of the oxidative burst by cells dying in the presence of oxidized low density lipoprotein[S]
Macrophages ingesting apoptotic cells attenuate inflammatory responses, such as reactive oxygen species (ROS) generation. In atherosclerosis, ongoing inflammation and accumulation of apoptotic/necrotic material are observed, suggesting defects of phagocytes in recognizing or responding to dying cell...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2009-11-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520306830 |
| _version_ | 1819127482316488704 |
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| author | Dmitry Namgaladze Carla Jennewein Stefan Preiss Andreas von Knethen Bernhard Brüne |
| author_facet | Dmitry Namgaladze Carla Jennewein Stefan Preiss Andreas von Knethen Bernhard Brüne |
| author_sort | Dmitry Namgaladze |
| collection | DOAJ |
| description | Macrophages ingesting apoptotic cells attenuate inflammatory responses, such as reactive oxygen species (ROS) generation. In atherosclerosis, ongoing inflammation and accumulation of apoptotic/necrotic material are observed, suggesting defects of phagocytes in recognizing or responding to dying cells. Modified lipoproteins such as oxidized LDL (oxLDL) are known to promote inflammation and to interfere with apoptotic cell clearance. Here, we studied the impact of cells exposed to oxLDL on their ability to interfere with the oxidative burst in phagocytes. In contrast to apoptotic cells, cells dying in response to or in the presence of oxLDL failed to suppress ROS generation despite efficiently being taken up by phagocytes. In addition, apoptotic cells, but not oxLDL-treated cells, inhibited phosphorylation of extracellular signal-regulated kinase, which is important for NADPH oxidase activation. oxLDL treatment did not interfere with activation of the antiinflammatory transcriptional regulator peroxisome proliferator-activated receptor γ by apoptotic cells. Moreover, cells exposed to oxLDL failed to suppress lipopolysaccharide- induced proinflammatory cytokine expression, whereas apoptotic cells attenuated these phagocyte responses. Thus, the presence of oxLDL during cell death impaired the ability of apoptotic cells to act antiinflammatory with regard to oxidative burst inhibition and cytokine expression in phagocytes. |
| first_indexed | 2024-12-22T08:12:38Z |
| format | Article |
| id | doaj.art-7a96e0c22cdf454a84429a0270de211c |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-22T08:12:38Z |
| publishDate | 2009-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-7a96e0c22cdf454a84429a0270de211c2022-12-21T18:32:59ZengElsevierJournal of Lipid Research0022-22752009-11-01501121732181Attenuated suppression of the oxidative burst by cells dying in the presence of oxidized low density lipoprotein[S]Dmitry Namgaladze0Carla Jennewein1Stefan Preiss2Andreas von Knethen3Bernhard Brüne4Goethe-University, Faculty of Medicine, Institute of Biochemistry I/ZAFES, 60590 Frankfurt, GermanyGoethe-University, Faculty of Medicine, Institute of Biochemistry I/ZAFES, 60590 Frankfurt, GermanyGoethe-University, Faculty of Medicine, Institute of Biochemistry I/ZAFES, 60590 Frankfurt, GermanyGoethe-University, Faculty of Medicine, Institute of Biochemistry I/ZAFES, 60590 Frankfurt, GermanyTo whom correspondence should be addressed; Goethe-University, Faculty of Medicine, Institute of Biochemistry I/ZAFES, 60590 Frankfurt, GermanyMacrophages ingesting apoptotic cells attenuate inflammatory responses, such as reactive oxygen species (ROS) generation. In atherosclerosis, ongoing inflammation and accumulation of apoptotic/necrotic material are observed, suggesting defects of phagocytes in recognizing or responding to dying cells. Modified lipoproteins such as oxidized LDL (oxLDL) are known to promote inflammation and to interfere with apoptotic cell clearance. Here, we studied the impact of cells exposed to oxLDL on their ability to interfere with the oxidative burst in phagocytes. In contrast to apoptotic cells, cells dying in response to or in the presence of oxLDL failed to suppress ROS generation despite efficiently being taken up by phagocytes. In addition, apoptotic cells, but not oxLDL-treated cells, inhibited phosphorylation of extracellular signal-regulated kinase, which is important for NADPH oxidase activation. oxLDL treatment did not interfere with activation of the antiinflammatory transcriptional regulator peroxisome proliferator-activated receptor γ by apoptotic cells. Moreover, cells exposed to oxLDL failed to suppress lipopolysaccharide- induced proinflammatory cytokine expression, whereas apoptotic cells attenuated these phagocyte responses. Thus, the presence of oxLDL during cell death impaired the ability of apoptotic cells to act antiinflammatory with regard to oxidative burst inhibition and cytokine expression in phagocytes.http://www.sciencedirect.com/science/article/pii/S0022227520306830apoptosismacrophagesNADPH oxidaselipoproteinsphagocytosisreactive oxygen species |
| spellingShingle | Dmitry Namgaladze Carla Jennewein Stefan Preiss Andreas von Knethen Bernhard Brüne Attenuated suppression of the oxidative burst by cells dying in the presence of oxidized low density lipoprotein[S] Journal of Lipid Research apoptosis macrophages NADPH oxidase lipoproteins phagocytosis reactive oxygen species |
| title | Attenuated suppression of the oxidative burst by cells dying in the presence of oxidized low density lipoprotein[S] |
| title_full | Attenuated suppression of the oxidative burst by cells dying in the presence of oxidized low density lipoprotein[S] |
| title_fullStr | Attenuated suppression of the oxidative burst by cells dying in the presence of oxidized low density lipoprotein[S] |
| title_full_unstemmed | Attenuated suppression of the oxidative burst by cells dying in the presence of oxidized low density lipoprotein[S] |
| title_short | Attenuated suppression of the oxidative burst by cells dying in the presence of oxidized low density lipoprotein[S] |
| title_sort | attenuated suppression of the oxidative burst by cells dying in the presence of oxidized low density lipoprotein s |
| topic | apoptosis macrophages NADPH oxidase lipoproteins phagocytosis reactive oxygen species |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520306830 |
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