Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in Sicily
A proliferative cauliflower lesion was excised from the udder of a sheep. Histological investigation confirmed the macroscopic classification of the lesion as a papilloma, without any fibroblastic proliferation. PCR revealed the presence of bovine papillomavirus (BPV), which was further confirmed by...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/6/429 |
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| author | Federica Savini Laura Gallina Alice Prosperi Roberto Puleio Antonio Lavazza Patrizia Di Marco Serena Tumino Ana Moreno Davide Lelli Annalisa Guercio Alessandra Scagliarini |
| author_facet | Federica Savini Laura Gallina Alice Prosperi Roberto Puleio Antonio Lavazza Patrizia Di Marco Serena Tumino Ana Moreno Davide Lelli Annalisa Guercio Alessandra Scagliarini |
| author_sort | Federica Savini |
| collection | DOAJ |
| description | A proliferative cauliflower lesion was excised from the udder of a sheep. Histological investigation confirmed the macroscopic classification of the lesion as a papilloma, without any fibroblastic proliferation. PCR revealed the presence of bovine papillomavirus (BPV), which was further confirmed by the identification of a <i>Deltapapillomavirus</i> <i>4</i> by Next Generation Sequencing analysis. This was subsequently classified as bovine papillomavirus type 1. Negative staining electron microscopy (EM) analyses produced negative test results for papillomavirus particles. RNA in situ hybridization (ISH) confirmed the presence of BPV-1. The results further confirm the ability of BPVs belonging to the <i>Deltapapillomavirus</i> genus to infect distantly related species and to cause lesions that are different from sarcoids. |
| first_indexed | 2024-03-10T19:29:44Z |
| format | Article |
| id | doaj.art-7a970071b92347769631f3c63395c28f |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T19:29:44Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-7a970071b92347769631f3c63395c28f2023-11-20T02:16:44ZengMDPI AGPathogens2076-08172020-05-019642910.3390/pathogens9060429Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in SicilyFederica Savini0Laura Gallina1Alice Prosperi2Roberto Puleio3Antonio Lavazza4Patrizia Di Marco5Serena Tumino6Ana Moreno7Davide Lelli8Annalisa Guercio9Alessandra Scagliarini10Dipartimento di Scienze Mediche Veterinarie, Università di Bologna, Ozzano Emilia, 40064 Bologna, ItalyDipartimento di Scienze Mediche Veterinarie, Università di Bologna, Ozzano Emilia, 40064 Bologna, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini”, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini”, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyDipartimento agricoltura, alimentazione, ambiente, sezione Produzioni animali, Università di Catania, 00159 Catania, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini”, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini”, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyDipartimento di Scienze Mediche Veterinarie, Università di Bologna, Ozzano Emilia, 40064 Bologna, ItalyA proliferative cauliflower lesion was excised from the udder of a sheep. Histological investigation confirmed the macroscopic classification of the lesion as a papilloma, without any fibroblastic proliferation. PCR revealed the presence of bovine papillomavirus (BPV), which was further confirmed by the identification of a <i>Deltapapillomavirus</i> <i>4</i> by Next Generation Sequencing analysis. This was subsequently classified as bovine papillomavirus type 1. Negative staining electron microscopy (EM) analyses produced negative test results for papillomavirus particles. RNA in situ hybridization (ISH) confirmed the presence of BPV-1. The results further confirm the ability of BPVs belonging to the <i>Deltapapillomavirus</i> genus to infect distantly related species and to cause lesions that are different from sarcoids.https://www.mdpi.com/2076-0817/9/6/429papillomavirussheepNGSwart |
| spellingShingle | Federica Savini Laura Gallina Alice Prosperi Roberto Puleio Antonio Lavazza Patrizia Di Marco Serena Tumino Ana Moreno Davide Lelli Annalisa Guercio Alessandra Scagliarini Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in Sicily Pathogens papillomavirus sheep NGS wart |
| title | Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in Sicily |
| title_full | Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in Sicily |
| title_fullStr | Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in Sicily |
| title_full_unstemmed | Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in Sicily |
| title_short | Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in Sicily |
| title_sort | bovine papillomavirus 1 gets out of the flock detection in an ovine wart in sicily |
| topic | papillomavirus sheep NGS wart |
| url | https://www.mdpi.com/2076-0817/9/6/429 |
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