Distribution of Apolipoprotein A1 Polymorphism (G-75A and C+83T) in Patients with Diabetic Foot Ulcers- A Parallel Group Hospital Based Observational Study
Introduction: Diabetic Foot Ulcer (DFU), a serious complications of diabetes mellitus is a result of persistent low grade infection. The Apolipoprotein A1 (ApoA1) has an anti-inflammatory role and therefore can influence the chronic inflammation associated with the DFU. Polymorphisms of ApoA1gen...
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JCDR Research and Publications Private Limited
2021-07-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/15160/48456_CE[Ra1]_F(SHU)_PF1(SY_RK)_PFA_NC(SY_KM)_PN(KM).pdf |
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| author | Rachita Nanda Suprava Patel Preetam Wasnik Radhakrishna Ramchandani Debajyoti Mohanty Eli Mohapatra |
| author_facet | Rachita Nanda Suprava Patel Preetam Wasnik Radhakrishna Ramchandani Debajyoti Mohanty Eli Mohapatra |
| author_sort | Rachita Nanda |
| collection | DOAJ |
| description | Introduction: Diabetic Foot Ulcer (DFU), a serious complications
of diabetes mellitus is a result of persistent low grade infection.
The Apolipoprotein A1 (ApoA1) has an anti-inflammatory role and
therefore can influence the chronic inflammation associated with
the DFU. Polymorphisms of ApoA1gene have been implicated
as determinants of plasma High-Density Lipoprotein cholesterol
(HDL-C) and Apo A1 levels. However, the influence of ApoA1
polymorphism on susceptibility to DFU has not been studied.
Aim: To study the distribution of ApoA1 polymorphism (G-75A
and C+83T) and association between the polymorphism and
the risk of DFU in patients with Type 2 Diabetes Mellitus (T2DM)
so that timely detection and prevention of DFU can be done.
Materials and Methods: This was a hospital based
observational study on 80 patients of DFU, 80 diabetes mellitus
without ulcers and 75 normal controls. ApoA1 polymorphism
(G-75A and C+83T) was detected by Real Time Polymerase
Chain Reaction (RT-PCR) technique and plasma ApoA1 by
immunoturbidimetric assay using blood collected in EDTA.
Data was analysed using IBM® Statistical Package for Social
Sciences (SPSS) 21.0 software. A p<0.05 was considered as
statistically significant.
Results: The GA and CC were the most predominant genotype
in all the groups. HDL and ApoA1 were significantly lower in
GG (p=0.009, p=0.03) and CT (p=0.03, p=0.002) compared
to GA and CC. The APOA1-75A allele and +83C allele were
associated with raised levels of HDL and ApoA1 in T2DM and
DFU (p<0.05).
Conclusion: The two polymorphism G-75A and C+83T were
found to be equally distributed across the study populations.
These polymorphisms were associated with serum levels of
ApoA1 and HDL in the DFU patients. |
| first_indexed | 2024-12-17T20:54:05Z |
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| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-17T20:54:05Z |
| publishDate | 2021-07-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-7a9989d63b1440288a92837e2ee968c92022-12-21T21:32:55ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2021-07-011507091410.7860/JCDR/2021/48456.15160Distribution of Apolipoprotein A1 Polymorphism (G-75A and C+83T) in Patients with Diabetic Foot Ulcers- A Parallel Group Hospital Based Observational StudyRachita Nanda0Suprava Patel1Preetam Wasnik2Radhakrishna Ramchandani3Debajyoti Mohanty4Eli Mohapatra5Additional Professor, Department of Biochemistry, All India Institute of Medical Sciences, Raipur, Chhattisgarh, India Associate Professor, Department of Biochemistry, All India Institute of Medical Sciences, Raipur, Chhattisgarh, India Associate Professor, Department of Medicine, All India Institute of Medical Sciences, Raipur, Chhattisgarh, IndiaAssociate Professor, Department of Surgery, All India Institute of Medical Sciences, Raipur, Chhattisgarh, IndiaAdditional Professor, Department of Surgery, All India Institute of Medical Sciences, Raipur, Chhattisgarh, India Professor and Head, Department of Biochemistry, All India Institute of Medical Sciences, Raipur, Chhattisgarh, India.Introduction: Diabetic Foot Ulcer (DFU), a serious complications of diabetes mellitus is a result of persistent low grade infection. The Apolipoprotein A1 (ApoA1) has an anti-inflammatory role and therefore can influence the chronic inflammation associated with the DFU. Polymorphisms of ApoA1gene have been implicated as determinants of plasma High-Density Lipoprotein cholesterol (HDL-C) and Apo A1 levels. However, the influence of ApoA1 polymorphism on susceptibility to DFU has not been studied. Aim: To study the distribution of ApoA1 polymorphism (G-75A and C+83T) and association between the polymorphism and the risk of DFU in patients with Type 2 Diabetes Mellitus (T2DM) so that timely detection and prevention of DFU can be done. Materials and Methods: This was a hospital based observational study on 80 patients of DFU, 80 diabetes mellitus without ulcers and 75 normal controls. ApoA1 polymorphism (G-75A and C+83T) was detected by Real Time Polymerase Chain Reaction (RT-PCR) technique and plasma ApoA1 by immunoturbidimetric assay using blood collected in EDTA. Data was analysed using IBM® Statistical Package for Social Sciences (SPSS) 21.0 software. A p<0.05 was considered as statistically significant. Results: The GA and CC were the most predominant genotype in all the groups. HDL and ApoA1 were significantly lower in GG (p=0.009, p=0.03) and CT (p=0.03, p=0.002) compared to GA and CC. The APOA1-75A allele and +83C allele were associated with raised levels of HDL and ApoA1 in T2DM and DFU (p<0.05). Conclusion: The two polymorphism G-75A and C+83T were found to be equally distributed across the study populations. These polymorphisms were associated with serum levels of ApoA1 and HDL in the DFU patients.https://jcdr.net/articles/PDF/15160/48456_CE[Ra1]_F(SHU)_PF1(SY_RK)_PFA_NC(SY_KM)_PN(KM).pdfalleleanti-inflammatorydyslipidaemiagenotypehigh density lipoprotein |
| spellingShingle | Rachita Nanda Suprava Patel Preetam Wasnik Radhakrishna Ramchandani Debajyoti Mohanty Eli Mohapatra Distribution of Apolipoprotein A1 Polymorphism (G-75A and C+83T) in Patients with Diabetic Foot Ulcers- A Parallel Group Hospital Based Observational Study Journal of Clinical and Diagnostic Research allele anti-inflammatory dyslipidaemia genotype high density lipoprotein |
| title | Distribution of Apolipoprotein A1 Polymorphism (G-75A and C+83T) in Patients with Diabetic Foot Ulcers- A Parallel Group Hospital Based Observational Study |
| title_full | Distribution of Apolipoprotein A1 Polymorphism (G-75A and C+83T) in Patients with Diabetic Foot Ulcers- A Parallel Group Hospital Based Observational Study |
| title_fullStr | Distribution of Apolipoprotein A1 Polymorphism (G-75A and C+83T) in Patients with Diabetic Foot Ulcers- A Parallel Group Hospital Based Observational Study |
| title_full_unstemmed | Distribution of Apolipoprotein A1 Polymorphism (G-75A and C+83T) in Patients with Diabetic Foot Ulcers- A Parallel Group Hospital Based Observational Study |
| title_short | Distribution of Apolipoprotein A1 Polymorphism (G-75A and C+83T) in Patients with Diabetic Foot Ulcers- A Parallel Group Hospital Based Observational Study |
| title_sort | distribution of apolipoprotein a1 polymorphism g 75a and c 83t in patients with diabetic foot ulcers a parallel group hospital based observational study |
| topic | allele anti-inflammatory dyslipidaemia genotype high density lipoprotein |
| url | https://jcdr.net/articles/PDF/15160/48456_CE[Ra1]_F(SHU)_PF1(SY_RK)_PFA_NC(SY_KM)_PN(KM).pdf |
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