Effect of artificial dsRNA on infection of pea plants by Pea seed-borne mosaic virus
The effect of direct application of artificial dsRNA molecules derived from the viral coat protein gene and the possibility of RNA interference induction leading to protection against Pea seed borne mosaic virus (PSbMV) have been investigated. Effectivity of virus transmission was not afected by the...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2014-06-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-201402-0010_effect-of-artificial-dsrna-on-infection-of-pea-plants-by-pea-seed-borne-mosaic-virus.php |
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| author | Dana ŠAFÁŘOVÁ Pavel BRÁZDA Milan NAVRÁTIL |
| author_facet | Dana ŠAFÁŘOVÁ Pavel BRÁZDA Milan NAVRÁTIL |
| author_sort | Dana ŠAFÁŘOVÁ |
| collection | DOAJ |
| description | The effect of direct application of artificial dsRNA molecules derived from the viral coat protein gene and the possibility of RNA interference induction leading to protection against Pea seed borne mosaic virus (PSbMV) have been investigated. Effectivity of virus transmission was not afected by the dsRNA treatment. Simultaneous application of dsRNA and virus inoculation as well as dsRNA application one day before PSbMV inoculation led to a significant short-term decrease in the PSbMV concentration in experimental pea plants. Either the curative effect of the artificial dsRNA biolistic application was not noticed. |
| first_indexed | 2024-04-10T08:29:28Z |
| format | Article |
| id | doaj.art-7a99eea418c14288b1a81bc0db739d68 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:29:28Z |
| publishDate | 2014-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-7a99eea418c14288b1a81bc0db739d682023-02-23T03:30:25ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252014-06-0150210510810.17221/120/2013-CJGPBcjg-201402-0010Effect of artificial dsRNA on infection of pea plants by Pea seed-borne mosaic virusDana ŠAFÁŘOVÁ0Pavel BRÁZDA1Milan NAVRÁTIL2Department of Cell Biology and Genetics, Faculty of Science, Palacký University Olomouc, Olomouc, Czech RepublicDepartment of Cell Biology and Genetics, Faculty of Science, Palacký University Olomouc, Olomouc, Czech RepublicDepartment of Cell Biology and Genetics, Faculty of Science, Palacký University Olomouc, Olomouc, Czech RepublicThe effect of direct application of artificial dsRNA molecules derived from the viral coat protein gene and the possibility of RNA interference induction leading to protection against Pea seed borne mosaic virus (PSbMV) have been investigated. Effectivity of virus transmission was not afected by the dsRNA treatment. Simultaneous application of dsRNA and virus inoculation as well as dsRNA application one day before PSbMV inoculation led to a significant short-term decrease in the PSbMV concentration in experimental pea plants. Either the curative effect of the artificial dsRNA biolistic application was not noticed.https://cjgpb.agriculturejournals.cz/artkey/cjg-201402-0010_effect-of-artificial-dsrna-on-infection-of-pea-plants-by-pea-seed-borne-mosaic-virus.phppisum sativumpsbmvrna vaccineqrt-pcr |
| spellingShingle | Dana ŠAFÁŘOVÁ Pavel BRÁZDA Milan NAVRÁTIL Effect of artificial dsRNA on infection of pea plants by Pea seed-borne mosaic virus Czech Journal of Genetics and Plant Breeding pisum sativum psbmv rna vaccine qrt-pcr |
| title | Effect of artificial dsRNA on infection of pea plants by Pea seed-borne mosaic virus |
| title_full | Effect of artificial dsRNA on infection of pea plants by Pea seed-borne mosaic virus |
| title_fullStr | Effect of artificial dsRNA on infection of pea plants by Pea seed-borne mosaic virus |
| title_full_unstemmed | Effect of artificial dsRNA on infection of pea plants by Pea seed-borne mosaic virus |
| title_short | Effect of artificial dsRNA on infection of pea plants by Pea seed-borne mosaic virus |
| title_sort | effect of artificial dsrna on infection of pea plants by pea seed borne mosaic virus |
| topic | pisum sativum psbmv rna vaccine qrt-pcr |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-201402-0010_effect-of-artificial-dsrna-on-infection-of-pea-plants-by-pea-seed-borne-mosaic-virus.php |
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