Antioxidant <i>Baccharis trimera</i> Leaf Extract Suppresses Lipid Accumulation in <i>C. elegans</i> Dependent on Transcription Factor NHR-49
Obesity is a global public health problem that is associated with oxidative stress. One of the strategies for the treatment of obesity is the use of drugs; however, these are expensive and have numerous side effects. Therefore, the search for new alternatives is necessary. <i>Baccharis trimera...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/10/1913 |
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| author | Flávia Roberta Monteiro Souza Giovanna Melo Martins Silva Cesar Orlando Muñoz Cadavid Lucas dos Santos Lisboa Maylla Maria Correia Leite Silva Weslley Souza Paiva Marcelo José Pena Ferreira Riva de Paula Oliveira Hugo Alexandre Oliveira Rocha |
| author_facet | Flávia Roberta Monteiro Souza Giovanna Melo Martins Silva Cesar Orlando Muñoz Cadavid Lucas dos Santos Lisboa Maylla Maria Correia Leite Silva Weslley Souza Paiva Marcelo José Pena Ferreira Riva de Paula Oliveira Hugo Alexandre Oliveira Rocha |
| author_sort | Flávia Roberta Monteiro Souza |
| collection | DOAJ |
| description | Obesity is a global public health problem that is associated with oxidative stress. One of the strategies for the treatment of obesity is the use of drugs; however, these are expensive and have numerous side effects. Therefore, the search for new alternatives is necessary. <i>Baccharis trimera</i> is used in Brazilian folk medicine for the treatment of obesity. Here, <i>B. trimera</i> leaf extract (BT) showed antioxidant activity in seven in vitro tests, and it was not toxic to 3T3 murine fibroblasts or <i>Caenorhabditis elegans</i>. Furthermore, BT reduces the intracellular amount of reactive oxygen species and increases <i>C. elegans</i> survival. Moreover, these effects were not dependent on transcription factors. The inhibition of fat accumulation by BT in the <i>C. elegans</i> model was also investigated. BT reduced lipid accumulation in animals fed diets without or with high amount of glucose. Furthermore, it was observed using RNA interference (iRNA) that BT depends on the transcription factor NHR-49 to exert its effect. Phytochemical analysis of BT revealed rutin, hyperoside, and 5-caffeoylquinic acid as the main BT components. Thus, these data demonstrate that BT has antioxidant and anti-obesity effects. However, further studies should be conducted to understand the mechanisms involved in its action. |
| first_indexed | 2024-03-09T20:49:39Z |
| format | Article |
| id | doaj.art-7a9e1d83e3dc4f4fa860c781cb60d77d |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T20:49:39Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-7a9e1d83e3dc4f4fa860c781cb60d77d2023-11-23T22:37:45ZengMDPI AGAntioxidants2076-39212022-09-011110191310.3390/antiox11101913Antioxidant <i>Baccharis trimera</i> Leaf Extract Suppresses Lipid Accumulation in <i>C. elegans</i> Dependent on Transcription Factor NHR-49Flávia Roberta Monteiro Souza0Giovanna Melo Martins Silva1Cesar Orlando Muñoz Cadavid2Lucas dos Santos Lisboa3Maylla Maria Correia Leite Silva4Weslley Souza Paiva5Marcelo José Pena Ferreira6Riva de Paula Oliveira7Hugo Alexandre Oliveira Rocha8Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Programa de Pós-graduação em Bioquímica e Biologia Molecular, Centro de Biociências, Federal University of Rio Grande do Norte—UFRN, Natal 59078-970, BrazilLaboratório de Genética Bioquímica (LGB), Programa de Pós-graduação em Biotecnologia, Centro de Biociências, Federal University of Rio Grande do Norte—UFRN, Natal 59078-970, BrazilLaboratório de Genética Bioquímica (LGB), Programa de Pós-graduação em Biotecnologia, Centro de Biociências, Federal University of Rio Grande do Norte—UFRN, Natal 59078-970, BrazilLaboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Programa de Pós-graduação em Bioquímica e Biologia Molecular, Centro de Biociências, Federal University of Rio Grande do Norte—UFRN, Natal 59078-970, BrazilLaboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Programa de Pós-graduação em Bioquímica e Biologia Molecular, Centro de Biociências, Federal University of Rio Grande do Norte—UFRN, Natal 59078-970, BrazilLaboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Programa de Pós-graduação em Bioquímica e Biologia Molecular, Centro de Biociências, Federal University of Rio Grande do Norte—UFRN, Natal 59078-970, BrazilLaboratório de Fitoquímica, Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo—USP, Rua do Matão, 277, São Paulo 05508-090, BrazilLaboratório de Genética Bioquímica (LGB), Programa de Pós-graduação em Biotecnologia, Centro de Biociências, Federal University of Rio Grande do Norte—UFRN, Natal 59078-970, BrazilLaboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Programa de Pós-graduação em Bioquímica e Biologia Molecular, Centro de Biociências, Federal University of Rio Grande do Norte—UFRN, Natal 59078-970, BrazilObesity is a global public health problem that is associated with oxidative stress. One of the strategies for the treatment of obesity is the use of drugs; however, these are expensive and have numerous side effects. Therefore, the search for new alternatives is necessary. <i>Baccharis trimera</i> is used in Brazilian folk medicine for the treatment of obesity. Here, <i>B. trimera</i> leaf extract (BT) showed antioxidant activity in seven in vitro tests, and it was not toxic to 3T3 murine fibroblasts or <i>Caenorhabditis elegans</i>. Furthermore, BT reduces the intracellular amount of reactive oxygen species and increases <i>C. elegans</i> survival. Moreover, these effects were not dependent on transcription factors. The inhibition of fat accumulation by BT in the <i>C. elegans</i> model was also investigated. BT reduced lipid accumulation in animals fed diets without or with high amount of glucose. Furthermore, it was observed using RNA interference (iRNA) that BT depends on the transcription factor NHR-49 to exert its effect. Phytochemical analysis of BT revealed rutin, hyperoside, and 5-caffeoylquinic acid as the main BT components. Thus, these data demonstrate that BT has antioxidant and anti-obesity effects. However, further studies should be conducted to understand the mechanisms involved in its action.https://www.mdpi.com/2076-3921/11/10/1913carquejaasteraceaephenolic compoundsobesityantioxidant<i>Caenorhabditis elegans</i> |
| spellingShingle | Flávia Roberta Monteiro Souza Giovanna Melo Martins Silva Cesar Orlando Muñoz Cadavid Lucas dos Santos Lisboa Maylla Maria Correia Leite Silva Weslley Souza Paiva Marcelo José Pena Ferreira Riva de Paula Oliveira Hugo Alexandre Oliveira Rocha Antioxidant <i>Baccharis trimera</i> Leaf Extract Suppresses Lipid Accumulation in <i>C. elegans</i> Dependent on Transcription Factor NHR-49 Antioxidants carqueja asteraceae phenolic compounds obesity antioxidant <i>Caenorhabditis elegans</i> |
| title | Antioxidant <i>Baccharis trimera</i> Leaf Extract Suppresses Lipid Accumulation in <i>C. elegans</i> Dependent on Transcription Factor NHR-49 |
| title_full | Antioxidant <i>Baccharis trimera</i> Leaf Extract Suppresses Lipid Accumulation in <i>C. elegans</i> Dependent on Transcription Factor NHR-49 |
| title_fullStr | Antioxidant <i>Baccharis trimera</i> Leaf Extract Suppresses Lipid Accumulation in <i>C. elegans</i> Dependent on Transcription Factor NHR-49 |
| title_full_unstemmed | Antioxidant <i>Baccharis trimera</i> Leaf Extract Suppresses Lipid Accumulation in <i>C. elegans</i> Dependent on Transcription Factor NHR-49 |
| title_short | Antioxidant <i>Baccharis trimera</i> Leaf Extract Suppresses Lipid Accumulation in <i>C. elegans</i> Dependent on Transcription Factor NHR-49 |
| title_sort | antioxidant i baccharis trimera i leaf extract suppresses lipid accumulation in i c elegans i dependent on transcription factor nhr 49 |
| topic | carqueja asteraceae phenolic compounds obesity antioxidant <i>Caenorhabditis elegans</i> |
| url | https://www.mdpi.com/2076-3921/11/10/1913 |
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