Characterization and expression of heat shock and immune genes in natural populations of Prodiamesa olivacea (Diptera) exposed to thermal stress
This paper characterizes the heat stress response (HSR) and explores the impact of temperatures on the immune response of larvae from two chironomid species, Prodiamesa olivacea and Chironomus riparius. Genes involved in crucial metabolic pathways were de novo identified in P. olivacea: Hsp27, Hsp60...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323008631 |
| _version_ | 1797683206099566592 |
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| author | Lola Llorente Mónica Aquilino Óscar Herrero Eduardo de la Peña Rosario Planelló |
| author_facet | Lola Llorente Mónica Aquilino Óscar Herrero Eduardo de la Peña Rosario Planelló |
| author_sort | Lola Llorente |
| collection | DOAJ |
| description | This paper characterizes the heat stress response (HSR) and explores the impact of temperatures on the immune response of larvae from two chironomid species, Prodiamesa olivacea and Chironomus riparius. Genes involved in crucial metabolic pathways were de novo identified in P. olivacea: Hsp27, Hsp60, Hsp70, Hsc70, Cdc37, and HSF for the heat stress response (HSR) and TOLL, PGRP, C-type lectin, and JAK/hopscotch for the immune system response (ISR). Quantitative real-time PCR was used to evaluate the expression levels of the selected genes in short-term treatments (up to 120') at high temperatures (35 °C and 39 °C). Exposing P. olivacea to elevated temperatures resulted in HSR induction with increased expression of specific heat shock genes, suggesting the potential of HSPs as early indicators of acute thermal stress. Surprisingly, we found that heat shock represses multiple immune genes, revealing the antagonist relation between the heat shock response and the innate immune response in P. olivacea. Our results also showed species-dependent gene responses, with more significant effects in P. olivacea, for most of the biomarkers studied, demonstrating a higher sensitivity in this species to environmental stress conditions than that of C. riparius. This work shows a multi-species approach that enables a deeper understanding of the effects of heat stress at the molecular level in aquatic dipterans. |
| first_indexed | 2024-03-12T00:11:11Z |
| format | Article |
| id | doaj.art-7aa00869bfd3454b87ca61086f53f72a |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-12T00:11:11Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-7aa00869bfd3454b87ca61086f53f72a2023-09-16T05:28:32ZengElsevierEcotoxicology and Environmental Safety0147-65132023-09-01263115359Characterization and expression of heat shock and immune genes in natural populations of Prodiamesa olivacea (Diptera) exposed to thermal stressLola Llorente0Mónica Aquilino1Óscar Herrero2Eduardo de la Peña3Rosario Planelló4Biology and Environmental Toxicology Group, Faculty of Science, Universidad Nacional de Educación a Distancia (UNED), 28232, Las Rozas, Madrid, SpainBiology and Environmental Toxicology Group, Faculty of Science, Universidad Nacional de Educación a Distancia (UNED), 28232, Las Rozas, Madrid, Spain; School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UKBiology and Environmental Toxicology Group, Faculty of Science, Universidad Nacional de Educación a Distancia (UNED), 28232, Las Rozas, Madrid, SpainInstitute for Subtropical and Mediterranean Horticulture (IHSM-UMA-CSIC), Spanish National Research Council (CSIC), Finca Experimental La Mayora, Algarrobo-Costa, 29750 Malaga, Spain; Department of Plants and Crops, Faculty of Bio-science Engineering, Ghent University, Coupure Links 653, Ghent 9000, BelgiumBiology and Environmental Toxicology Group, Faculty of Science, Universidad Nacional de Educación a Distancia (UNED), 28232, Las Rozas, Madrid, Spain; Corresponding author.This paper characterizes the heat stress response (HSR) and explores the impact of temperatures on the immune response of larvae from two chironomid species, Prodiamesa olivacea and Chironomus riparius. Genes involved in crucial metabolic pathways were de novo identified in P. olivacea: Hsp27, Hsp60, Hsp70, Hsc70, Cdc37, and HSF for the heat stress response (HSR) and TOLL, PGRP, C-type lectin, and JAK/hopscotch for the immune system response (ISR). Quantitative real-time PCR was used to evaluate the expression levels of the selected genes in short-term treatments (up to 120') at high temperatures (35 °C and 39 °C). Exposing P. olivacea to elevated temperatures resulted in HSR induction with increased expression of specific heat shock genes, suggesting the potential of HSPs as early indicators of acute thermal stress. Surprisingly, we found that heat shock represses multiple immune genes, revealing the antagonist relation between the heat shock response and the innate immune response in P. olivacea. Our results also showed species-dependent gene responses, with more significant effects in P. olivacea, for most of the biomarkers studied, demonstrating a higher sensitivity in this species to environmental stress conditions than that of C. riparius. This work shows a multi-species approach that enables a deeper understanding of the effects of heat stress at the molecular level in aquatic dipterans.http://www.sciencedirect.com/science/article/pii/S0147651323008631ChironomidsTranscriptional biomarkersImmune system responseHeat shock responseGlobal changeSentinel organism |
| spellingShingle | Lola Llorente Mónica Aquilino Óscar Herrero Eduardo de la Peña Rosario Planelló Characterization and expression of heat shock and immune genes in natural populations of Prodiamesa olivacea (Diptera) exposed to thermal stress Ecotoxicology and Environmental Safety Chironomids Transcriptional biomarkers Immune system response Heat shock response Global change Sentinel organism |
| title | Characterization and expression of heat shock and immune genes in natural populations of Prodiamesa olivacea (Diptera) exposed to thermal stress |
| title_full | Characterization and expression of heat shock and immune genes in natural populations of Prodiamesa olivacea (Diptera) exposed to thermal stress |
| title_fullStr | Characterization and expression of heat shock and immune genes in natural populations of Prodiamesa olivacea (Diptera) exposed to thermal stress |
| title_full_unstemmed | Characterization and expression of heat shock and immune genes in natural populations of Prodiamesa olivacea (Diptera) exposed to thermal stress |
| title_short | Characterization and expression of heat shock and immune genes in natural populations of Prodiamesa olivacea (Diptera) exposed to thermal stress |
| title_sort | characterization and expression of heat shock and immune genes in natural populations of prodiamesa olivacea diptera exposed to thermal stress |
| topic | Chironomids Transcriptional biomarkers Immune system response Heat shock response Global change Sentinel organism |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323008631 |
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