Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike Protein
To date, SARS-CoV-2 pandemic has caused more than 188 million infections and 4.06 million deaths worldwide. The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein has been regarded as an important target for vaccine and therapeutics development because it plays a key role in binding the h...
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MDPI AG
2021-07-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/9/8/829 |
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| author | Junping Hong Qian Wang Qian Wu Junyu Chen Xijing Wang Yingbin Wang Yixin Chen Ningshao Xia |
| author_facet | Junping Hong Qian Wang Qian Wu Junyu Chen Xijing Wang Yingbin Wang Yixin Chen Ningshao Xia |
| author_sort | Junping Hong |
| collection | DOAJ |
| description | To date, SARS-CoV-2 pandemic has caused more than 188 million infections and 4.06 million deaths worldwide. The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein has been regarded as an important target for vaccine and therapeutics development because it plays a key role in binding the human cell receptor ACE2 that is required for viral entry. However, it is not easy to detect RBD in Western blot using polyclonal antibody, suggesting that RBD may form a complicated conformation under native condition and bear rare linear epitope. So far, no linear epitope on RBD is reported. Thus, a monoclonal antibody (mAb) that recognizes linear epitope on RBD will become valuable. In the present study, an RBD-specific rabbit antibody named 9E1 was isolated from peripheral blood mononuclear cells (PBMC) of immunized rabbit by RBD-specific single B cell sorting and mapped to a highly conserved linear epitope within twelve amino acids <sup>480</sup>CNGVEGFNCYFP<sup>491</sup> on RBD. 9E1 works well in Western blot on S protein and immunohistochemistry on the SARS-CoV-2 infected tissue sections. The results demonstrated that 9E1 can be used as a useful tool for pathological and functional studies of SARS-CoV-2. |
| first_indexed | 2024-03-10T08:19:38Z |
| format | Article |
| id | doaj.art-7aa1eed6c98c45a182c4bd1942026637 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T08:19:38Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-7aa1eed6c98c45a182c4bd19420266372023-11-22T10:06:33ZengMDPI AGVaccines2076-393X2021-07-019882910.3390/vaccines9080829Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike ProteinJunping Hong0Qian Wang1Qian Wu2Junyu Chen3Xijing Wang4Yingbin Wang5Yixin Chen6Ningshao Xia7National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen 361102, ChinaNational Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen 361102, ChinaNational Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, ChinaNational Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, ChinaNational Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen 361102, ChinaNational Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen 361102, ChinaTo date, SARS-CoV-2 pandemic has caused more than 188 million infections and 4.06 million deaths worldwide. The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein has been regarded as an important target for vaccine and therapeutics development because it plays a key role in binding the human cell receptor ACE2 that is required for viral entry. However, it is not easy to detect RBD in Western blot using polyclonal antibody, suggesting that RBD may form a complicated conformation under native condition and bear rare linear epitope. So far, no linear epitope on RBD is reported. Thus, a monoclonal antibody (mAb) that recognizes linear epitope on RBD will become valuable. In the present study, an RBD-specific rabbit antibody named 9E1 was isolated from peripheral blood mononuclear cells (PBMC) of immunized rabbit by RBD-specific single B cell sorting and mapped to a highly conserved linear epitope within twelve amino acids <sup>480</sup>CNGVEGFNCYFP<sup>491</sup> on RBD. 9E1 works well in Western blot on S protein and immunohistochemistry on the SARS-CoV-2 infected tissue sections. The results demonstrated that 9E1 can be used as a useful tool for pathological and functional studies of SARS-CoV-2.https://www.mdpi.com/2076-393X/9/8/829SARS-CoV-2spike proteinRBDrabbit monoclonal antibodyIHClinear B-cell epitope |
| spellingShingle | Junping Hong Qian Wang Qian Wu Junyu Chen Xijing Wang Yingbin Wang Yixin Chen Ningshao Xia Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike Protein Vaccines SARS-CoV-2 spike protein RBD rabbit monoclonal antibody IHC linear B-cell epitope |
| title | Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike Protein |
| title_full | Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike Protein |
| title_fullStr | Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike Protein |
| title_full_unstemmed | Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike Protein |
| title_short | Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike Protein |
| title_sort | rabbit monoclonal antibody specifically recognizing a linear epitope in the rbd of sars cov 2 spike protein |
| topic | SARS-CoV-2 spike protein RBD rabbit monoclonal antibody IHC linear B-cell epitope |
| url | https://www.mdpi.com/2076-393X/9/8/829 |
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