The effect of temperature on the diapause and cold hardiness of Dendrolimus tabulaeformis (Lepidoptera: Lasiocampidae)
Pine caterpillar, Dendrolimus tabulaeformis Tsai et Liu, is a major pine pest in North China. The larvae enter diapause in the third or fourth instar before winter. Supercooling points (SCP) and cold hardiness of the diapausing larvae were investigated and compared in non-acclimated, acclimated and...
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Institute of Entomology, Biology Centre, Czech Academy of Science
2008-10-01
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| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-200804-0008_The_effect_of_temperature_on_the_diapause_and_cold_hardiness_of_Dendrolimus_tabulaeformis_Lepidoptera_Lasioca.php |
| _version_ | 1818655586565226496 |
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| author | Ju-Ping ZENG Feng GE Jian-Wei SU Yong WANG |
| author_facet | Ju-Ping ZENG Feng GE Jian-Wei SU Yong WANG |
| author_sort | Ju-Ping ZENG |
| collection | DOAJ |
| description | Pine caterpillar, Dendrolimus tabulaeformis Tsai et Liu, is a major pine pest in North China. The larvae enter diapause in the third or fourth instar before winter. Supercooling points (SCP) and cold hardiness of the diapausing larvae were investigated and compared in non-acclimated, acclimated and de-acclimated larvae. A bimodal frequency distribution was observed with a break point of -14°C in the SCP. Larvae in the low group (LG, SCP <= -14°C) were more cold tolerant with lower lethal temperatures than those in the high group (HG, SCP > -14°C). This bimodality occurred in three patterns, LG (> 60% of individuals in LG), LG-HG (< 60% of individuals in LG and HG) and HG (> 60% of individuals in HG), in response to cold acclimation and de-acclimation. The cold hardiness was ranked as: LG > LG-HG > HG pattern. Cold hardiness was enhanced by an increase in concentrations of trehalose, galactose, glucose and mannose in the haemolymph as well as by decrease in metabolism after cold acclimation, but was lost after de-acclimation. Loss of cold hardiness was correlated with decrease in sugars and increase in metabolic rate. In conclusion, the species is a chill tolerant insect, adopting the strategy of depressing SCP through accumulation of low molecular weight sugars in the haemolymph, concomitant with metabolic depression. |
| first_indexed | 2024-12-17T03:12:03Z |
| format | Article |
| id | doaj.art-7aa22ea8dd7244ecaf1c51412038b671 |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-17T03:12:03Z |
| publishDate | 2008-10-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-7aa22ea8dd7244ecaf1c51412038b6712022-12-21T22:05:48ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292008-10-01105459960610.14411/eje.2008.080eje-200804-0008The effect of temperature on the diapause and cold hardiness of Dendrolimus tabulaeformis (Lepidoptera: Lasiocampidae)Ju-Ping ZENG0Feng GE1Jian-Wei SU2Yong WANG3State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. ChinaState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. ChinaState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. ChinaHunan Agricultural University, Changsha 410128, P.R. ChinaPine caterpillar, Dendrolimus tabulaeformis Tsai et Liu, is a major pine pest in North China. The larvae enter diapause in the third or fourth instar before winter. Supercooling points (SCP) and cold hardiness of the diapausing larvae were investigated and compared in non-acclimated, acclimated and de-acclimated larvae. A bimodal frequency distribution was observed with a break point of -14°C in the SCP. Larvae in the low group (LG, SCP <= -14°C) were more cold tolerant with lower lethal temperatures than those in the high group (HG, SCP > -14°C). This bimodality occurred in three patterns, LG (> 60% of individuals in LG), LG-HG (< 60% of individuals in LG and HG) and HG (> 60% of individuals in HG), in response to cold acclimation and de-acclimation. The cold hardiness was ranked as: LG > LG-HG > HG pattern. Cold hardiness was enhanced by an increase in concentrations of trehalose, galactose, glucose and mannose in the haemolymph as well as by decrease in metabolism after cold acclimation, but was lost after de-acclimation. Loss of cold hardiness was correlated with decrease in sugars and increase in metabolic rate. In conclusion, the species is a chill tolerant insect, adopting the strategy of depressing SCP through accumulation of low molecular weight sugars in the haemolymph, concomitant with metabolic depression.https://www.eje.cz/artkey/eje-200804-0008_The_effect_of_temperature_on_the_diapause_and_cold_hardiness_of_Dendrolimus_tabulaeformis_Lepidoptera_Lasioca.phpdendrolimus tabulaeformisacclimationde-acclimationcold hardinesssupercooling pointlow molecular weight sugars |
| spellingShingle | Ju-Ping ZENG Feng GE Jian-Wei SU Yong WANG The effect of temperature on the diapause and cold hardiness of Dendrolimus tabulaeformis (Lepidoptera: Lasiocampidae) European Journal of Entomology dendrolimus tabulaeformis acclimation de-acclimation cold hardiness supercooling point low molecular weight sugars |
| title | The effect of temperature on the diapause and cold hardiness of Dendrolimus tabulaeformis (Lepidoptera: Lasiocampidae) |
| title_full | The effect of temperature on the diapause and cold hardiness of Dendrolimus tabulaeformis (Lepidoptera: Lasiocampidae) |
| title_fullStr | The effect of temperature on the diapause and cold hardiness of Dendrolimus tabulaeformis (Lepidoptera: Lasiocampidae) |
| title_full_unstemmed | The effect of temperature on the diapause and cold hardiness of Dendrolimus tabulaeformis (Lepidoptera: Lasiocampidae) |
| title_short | The effect of temperature on the diapause and cold hardiness of Dendrolimus tabulaeformis (Lepidoptera: Lasiocampidae) |
| title_sort | effect of temperature on the diapause and cold hardiness of dendrolimus tabulaeformis lepidoptera lasiocampidae |
| topic | dendrolimus tabulaeformis acclimation de-acclimation cold hardiness supercooling point low molecular weight sugars |
| url | https://www.eje.cz/artkey/eje-200804-0008_The_effect_of_temperature_on_the_diapause_and_cold_hardiness_of_Dendrolimus_tabulaeformis_Lepidoptera_Lasioca.php |
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