Detection of circulating and fecal Trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvae

Different assays to detect antigens of Trichinella spiralis during current infection have been standardized, although sensitivity values have been the main limitation to use them as routine diagnostic test. We report the production and use of monoclonal antibodies (MAbs) against the new born larvae...

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Main Authors: De-La-Rosa J.-L., Moran-Tlatelpa F., Medina Y., Gomez-Priego A., Correa D.
Format: Article
Language:English
Published: EDP Sciences 2001-06-01
Series:Parasite
Subjects:
Online Access:http://dx.doi.org/10.1051/parasite/200108s2123
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author De-La-Rosa J.-L.
Moran-Tlatelpa F.
Medina Y.
Gomez-Priego A.
Correa D.
author_facet De-La-Rosa J.-L.
Moran-Tlatelpa F.
Medina Y.
Gomez-Priego A.
Correa D.
author_sort De-La-Rosa J.-L.
collection DOAJ
description Different assays to detect antigens of Trichinella spiralis during current infection have been standardized, although sensitivity values have been the main limitation to use them as routine diagnostic test. We report the production and use of monoclonal antibodies (MAbs) against the new born larvae which recognize both somatic and metabolic antigens from adult and muscular larvae (ML). We used two lgG3 MAbs (4B1, 4B2) and two lgG2a (2D3, 2D4) to detect antigens during experimental infection. All MAbs detect fecal antigens starting the second to third week post infection (wpi), although it was less clear with MAbs 4B1 and 4B2, while circulating antigens were detected from third to fourth wpi. Thus, the recognition of shared antigens among T. spiralis developmental stages can be used for early diagnosis of trichinellosis.
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spelling doaj.art-7aa9908ce43b43e381275daf8eb5e8ff2023-12-02T12:49:58ZengEDP SciencesParasite1252-607X1776-10422001-06-018S123S12510.1051/parasite/200108s2123parasite200108s2p123Detection of circulating and fecal Trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvaeDe-La-Rosa J.-L.Moran-Tlatelpa F.Medina Y.Gomez-Priego A.Correa D.Different assays to detect antigens of Trichinella spiralis during current infection have been standardized, although sensitivity values have been the main limitation to use them as routine diagnostic test. We report the production and use of monoclonal antibodies (MAbs) against the new born larvae which recognize both somatic and metabolic antigens from adult and muscular larvae (ML). We used two lgG3 MAbs (4B1, 4B2) and two lgG2a (2D3, 2D4) to detect antigens during experimental infection. All MAbs detect fecal antigens starting the second to third week post infection (wpi), although it was less clear with MAbs 4B1 and 4B2, while circulating antigens were detected from third to fourth wpi. Thus, the recognition of shared antigens among T. spiralis developmental stages can be used for early diagnosis of trichinellosis.http://dx.doi.org/10.1051/parasite/200108s2123Trichinellaantigensmonoclonal-antibodydiagnosisnew born larva
spellingShingle De-La-Rosa J.-L.
Moran-Tlatelpa F.
Medina Y.
Gomez-Priego A.
Correa D.
Detection of circulating and fecal Trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvae
Parasite
Trichinella
antigens
monoclonal-antibody
diagnosis
new born larva
title Detection of circulating and fecal Trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvae
title_full Detection of circulating and fecal Trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvae
title_fullStr Detection of circulating and fecal Trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvae
title_full_unstemmed Detection of circulating and fecal Trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvae
title_short Detection of circulating and fecal Trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvae
title_sort detection of circulating and fecal trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvae
topic Trichinella
antigens
monoclonal-antibody
diagnosis
new born larva
url http://dx.doi.org/10.1051/parasite/200108s2123
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