Detection of circulating and fecal Trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvae
Different assays to detect antigens of Trichinella spiralis during current infection have been standardized, although sensitivity values have been the main limitation to use them as routine diagnostic test. We report the production and use of monoclonal antibodies (MAbs) against the new born larvae...
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Format: | Article |
Language: | English |
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EDP Sciences
2001-06-01
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Series: | Parasite |
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Online Access: | http://dx.doi.org/10.1051/parasite/200108s2123 |
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author | De-La-Rosa J.-L. Moran-Tlatelpa F. Medina Y. Gomez-Priego A. Correa D. |
author_facet | De-La-Rosa J.-L. Moran-Tlatelpa F. Medina Y. Gomez-Priego A. Correa D. |
author_sort | De-La-Rosa J.-L. |
collection | DOAJ |
description | Different assays to detect antigens of Trichinella spiralis during current infection have been standardized, although sensitivity values have been the main limitation to use them as routine diagnostic test. We report the production and use of monoclonal antibodies (MAbs) against the new born larvae which recognize both somatic and metabolic antigens from adult and muscular larvae (ML). We used two lgG3 MAbs (4B1, 4B2) and two lgG2a (2D3, 2D4) to detect antigens during experimental infection. All MAbs detect fecal antigens starting the second to third week post infection (wpi), although it was less clear with MAbs 4B1 and 4B2, while circulating antigens were detected from third to fourth wpi. Thus, the recognition of shared antigens among T. spiralis developmental stages can be used for early diagnosis of trichinellosis. |
first_indexed | 2024-03-09T08:57:15Z |
format | Article |
id | doaj.art-7aa9908ce43b43e381275daf8eb5e8ff |
institution | Directory Open Access Journal |
issn | 1252-607X 1776-1042 |
language | English |
last_indexed | 2024-03-09T08:57:15Z |
publishDate | 2001-06-01 |
publisher | EDP Sciences |
record_format | Article |
series | Parasite |
spelling | doaj.art-7aa9908ce43b43e381275daf8eb5e8ff2023-12-02T12:49:58ZengEDP SciencesParasite1252-607X1776-10422001-06-018S123S12510.1051/parasite/200108s2123parasite200108s2p123Detection of circulating and fecal Trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvaeDe-La-Rosa J.-L.Moran-Tlatelpa F.Medina Y.Gomez-Priego A.Correa D.Different assays to detect antigens of Trichinella spiralis during current infection have been standardized, although sensitivity values have been the main limitation to use them as routine diagnostic test. We report the production and use of monoclonal antibodies (MAbs) against the new born larvae which recognize both somatic and metabolic antigens from adult and muscular larvae (ML). We used two lgG3 MAbs (4B1, 4B2) and two lgG2a (2D3, 2D4) to detect antigens during experimental infection. All MAbs detect fecal antigens starting the second to third week post infection (wpi), although it was less clear with MAbs 4B1 and 4B2, while circulating antigens were detected from third to fourth wpi. Thus, the recognition of shared antigens among T. spiralis developmental stages can be used for early diagnosis of trichinellosis.http://dx.doi.org/10.1051/parasite/200108s2123Trichinellaantigensmonoclonal-antibodydiagnosisnew born larva |
spellingShingle | De-La-Rosa J.-L. Moran-Tlatelpa F. Medina Y. Gomez-Priego A. Correa D. Detection of circulating and fecal Trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvae Parasite Trichinella antigens monoclonal-antibody diagnosis new born larva |
title | Detection of circulating and fecal Trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvae |
title_full | Detection of circulating and fecal Trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvae |
title_fullStr | Detection of circulating and fecal Trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvae |
title_full_unstemmed | Detection of circulating and fecal Trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvae |
title_short | Detection of circulating and fecal Trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvae |
title_sort | detection of circulating and fecal trichinella spiralis antigens during experimental infection using monoclonal antibodies against the new born larvae |
topic | Trichinella antigens monoclonal-antibody diagnosis new born larva |
url | http://dx.doi.org/10.1051/parasite/200108s2123 |
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