Cell death dependent on holins LrgAB repressed by a novel ArsR family regulator CdsR
Abstract The cell death and survival paradox in various biological processes requires clarification. While spore development causes maternal cell death in Bacillus species, the involvement of other cell death pathways in sporulation remains unknown. Here, we identified a novel ArsR family transcript...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2024-04-01
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| Series: | Cell Death Discovery |
| Online Access: | https://doi.org/10.1038/s41420-024-01942-3 |
| _version_ | 1797209567202902016 |
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| author | Xin Zhang Yuhan Chen Tinglu Yan Hengjie Wang Ruibin Zhang Yanrong Xu Yujia Hou Qi Peng Fuping Song |
| author_facet | Xin Zhang Yuhan Chen Tinglu Yan Hengjie Wang Ruibin Zhang Yanrong Xu Yujia Hou Qi Peng Fuping Song |
| author_sort | Xin Zhang |
| collection | DOAJ |
| description | Abstract The cell death and survival paradox in various biological processes requires clarification. While spore development causes maternal cell death in Bacillus species, the involvement of other cell death pathways in sporulation remains unknown. Here, we identified a novel ArsR family transcriptional regulator, CdsR, and found that the deletion of its encoding gene cdsR causes cell lysis and inhibits sporulation. To our knowledge, this is the first report of an ArsR family transcriptional regulator governing cell death. We found that CdsR directly repressed lrgAB expression. Furthermore, lrgAB overexpression resulted in cell lysis without sporulation, akin to the cdsR mutant, suggesting that LrgAB, a holin-like protein, induces cell death in Bacillus spp. The lrgAB mutation increases abnormal cell numbers during spore development. In conclusion, we propose that a novel repressor is vital for inhibiting LrgAB-dependent cell lysis. |
| first_indexed | 2024-04-24T09:56:45Z |
| format | Article |
| id | doaj.art-7aad23ed000441b181b67d29781da3a4 |
| institution | Directory Open Access Journal |
| issn | 2058-7716 |
| language | English |
| last_indexed | 2024-04-24T09:56:45Z |
| publishDate | 2024-04-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Cell Death Discovery |
| spelling | doaj.art-7aad23ed000441b181b67d29781da3a42024-04-14T11:07:58ZengNature Publishing GroupCell Death Discovery2058-77162024-04-0110111310.1038/s41420-024-01942-3Cell death dependent on holins LrgAB repressed by a novel ArsR family regulator CdsRXin Zhang0Yuhan Chen1Tinglu Yan2Hengjie Wang3Ruibin Zhang4Yanrong Xu5Yujia Hou6Qi Peng7Fuping Song8State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesAbstract The cell death and survival paradox in various biological processes requires clarification. While spore development causes maternal cell death in Bacillus species, the involvement of other cell death pathways in sporulation remains unknown. Here, we identified a novel ArsR family transcriptional regulator, CdsR, and found that the deletion of its encoding gene cdsR causes cell lysis and inhibits sporulation. To our knowledge, this is the first report of an ArsR family transcriptional regulator governing cell death. We found that CdsR directly repressed lrgAB expression. Furthermore, lrgAB overexpression resulted in cell lysis without sporulation, akin to the cdsR mutant, suggesting that LrgAB, a holin-like protein, induces cell death in Bacillus spp. The lrgAB mutation increases abnormal cell numbers during spore development. In conclusion, we propose that a novel repressor is vital for inhibiting LrgAB-dependent cell lysis.https://doi.org/10.1038/s41420-024-01942-3 |
| spellingShingle | Xin Zhang Yuhan Chen Tinglu Yan Hengjie Wang Ruibin Zhang Yanrong Xu Yujia Hou Qi Peng Fuping Song Cell death dependent on holins LrgAB repressed by a novel ArsR family regulator CdsR Cell Death Discovery |
| title | Cell death dependent on holins LrgAB repressed by a novel ArsR family regulator CdsR |
| title_full | Cell death dependent on holins LrgAB repressed by a novel ArsR family regulator CdsR |
| title_fullStr | Cell death dependent on holins LrgAB repressed by a novel ArsR family regulator CdsR |
| title_full_unstemmed | Cell death dependent on holins LrgAB repressed by a novel ArsR family regulator CdsR |
| title_short | Cell death dependent on holins LrgAB repressed by a novel ArsR family regulator CdsR |
| title_sort | cell death dependent on holins lrgab repressed by a novel arsr family regulator cdsr |
| url | https://doi.org/10.1038/s41420-024-01942-3 |
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