SR5AL serves as a key regulatory gene in lycopene biosynthesis by Blakeslea trispora
Abstract Background Trisporic acids are considered to be key regulators of carotenoid biosynthesis and sexual reproduction in zygomycetes, but the mechanisms underlying this regulation have not been fully elucidated. Results In this study, the relationships between trisporic acids and lycopene synth...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-022-01853-x |
| _version_ | 1811227221906423808 |
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| author | Qiang Wang Yulong Chen Qingxiang Yang Jihong Zhao Lingran Feng Min Wang |
| author_facet | Qiang Wang Yulong Chen Qingxiang Yang Jihong Zhao Lingran Feng Min Wang |
| author_sort | Qiang Wang |
| collection | DOAJ |
| description | Abstract Background Trisporic acids are considered to be key regulators of carotenoid biosynthesis and sexual reproduction in zygomycetes, but the mechanisms underlying this regulation have not been fully elucidated. Results In this study, the relationships between trisporic acids and lycopene synthesis were investigated in Blakeslea trispora. The lycopene concentration in single fermentation by the (−) strain with the addition of 24 μg/L trisporic acids was slightly higher than that observed in mated fermentation. After transcriptomic analysis, a steroid 5α-reductase-like gene, known as SR5AL in B. trispora, was first reported. 5α-Reductase inhibitors reduced lycopene biosynthesis and downregulated the expression of sex determination and carotenoid biosynthesis genes. Overexpression of the SR5AL gene upregulated these genes, regardless of whether trisporic acids were added. Conclusion These findings indicated that the SR5AL gene is a key gene associated with the response to trisporic acids. |
| first_indexed | 2024-04-12T09:38:56Z |
| format | Article |
| id | doaj.art-7ab114315e16408db03ad85bd8742d8b |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-12T09:38:56Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-7ab114315e16408db03ad85bd8742d8b2022-12-22T03:38:09ZengBMCMicrobial Cell Factories1475-28592022-06-0121111010.1186/s12934-022-01853-xSR5AL serves as a key regulatory gene in lycopene biosynthesis by Blakeslea trisporaQiang Wang0Yulong Chen1Qingxiang Yang2Jihong Zhao3Lingran Feng4Min Wang5College of Life Sciences, Henan Normal UniversityCollege of Life Sciences, Henan Normal UniversityCollege of Life Sciences, Henan Normal UniversityCollege of Life Sciences, Henan Normal UniversityCollege of Life Sciences, Henan Normal UniversityCollege of Life Sciences, Henan Normal UniversityAbstract Background Trisporic acids are considered to be key regulators of carotenoid biosynthesis and sexual reproduction in zygomycetes, but the mechanisms underlying this regulation have not been fully elucidated. Results In this study, the relationships between trisporic acids and lycopene synthesis were investigated in Blakeslea trispora. The lycopene concentration in single fermentation by the (−) strain with the addition of 24 μg/L trisporic acids was slightly higher than that observed in mated fermentation. After transcriptomic analysis, a steroid 5α-reductase-like gene, known as SR5AL in B. trispora, was first reported. 5α-Reductase inhibitors reduced lycopene biosynthesis and downregulated the expression of sex determination and carotenoid biosynthesis genes. Overexpression of the SR5AL gene upregulated these genes, regardless of whether trisporic acids were added. Conclusion These findings indicated that the SR5AL gene is a key gene associated with the response to trisporic acids.https://doi.org/10.1186/s12934-022-01853-xTrisporic acidsLycopene biosynthesisSteroid 5α-reductaseSexM geneBlakeslea trispora |
| spellingShingle | Qiang Wang Yulong Chen Qingxiang Yang Jihong Zhao Lingran Feng Min Wang SR5AL serves as a key regulatory gene in lycopene biosynthesis by Blakeslea trispora Microbial Cell Factories Trisporic acids Lycopene biosynthesis Steroid 5α-reductase SexM gene Blakeslea trispora |
| title | SR5AL serves as a key regulatory gene in lycopene biosynthesis by Blakeslea trispora |
| title_full | SR5AL serves as a key regulatory gene in lycopene biosynthesis by Blakeslea trispora |
| title_fullStr | SR5AL serves as a key regulatory gene in lycopene biosynthesis by Blakeslea trispora |
| title_full_unstemmed | SR5AL serves as a key regulatory gene in lycopene biosynthesis by Blakeslea trispora |
| title_short | SR5AL serves as a key regulatory gene in lycopene biosynthesis by Blakeslea trispora |
| title_sort | sr5al serves as a key regulatory gene in lycopene biosynthesis by blakeslea trispora |
| topic | Trisporic acids Lycopene biosynthesis Steroid 5α-reductase SexM gene Blakeslea trispora |
| url | https://doi.org/10.1186/s12934-022-01853-x |
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