Thyroid Transcriptomics Revealed the Reproductive Regulation of miRNA in the Follicular and Luteal Phases in Small-Tail Han Sheep with Different FecB Genotypes
MicroRNA (miRNA) is a type of endogenous short−stranded ncRNA that influences many biological processes such as animal growth, development and metabolism. The thyroid gland is an important endocrine gland in sheep, and an increasing number of studies have shown that the thyroid gland plays an import...
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MDPI AG
2023-10-01
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| Series: | Genes |
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| author | Cheng Chang Xiaoyun He Ran Di Xiangyu Wang Miaoceng Han Chen Liang Mingxing Chu |
| author_facet | Cheng Chang Xiaoyun He Ran Di Xiangyu Wang Miaoceng Han Chen Liang Mingxing Chu |
| author_sort | Cheng Chang |
| collection | DOAJ |
| description | MicroRNA (miRNA) is a type of endogenous short−stranded ncRNA that influences many biological processes such as animal growth, development and metabolism. The thyroid gland is an important endocrine gland in sheep, and an increasing number of studies have shown that the thyroid gland plays an important role in animal reproduction, but the molecular mechanisms of the thyroid gland in sheep reproduction are poorly understood. In this study, RNA-seq was used to detect transcriptome expression patterns in the thyroid gland between the follicular phase (FP) and luteal phase (LP) in <i>FecB BB</i> (MM) and <i>FecB</i> ++ (ww) small-tail Han (STH) sheep, respectively, and to identify differentially expressed miRNAs (DEMs) associated with reproduction. Bioinformatic analysis of the target genes of these DEMs revealed that they can be enriched in multiple GO terms associated with the reproductive process in animals and in the KEGG signaling pathway. The miRNA–mRNA coexpression network revealed that oar-miR-133 and oar-miR-370-3p may play an important role in sheep reproduction. The results of the dual-luciferase reporter assay suggest a possible targeting relationship between novel-51 and <i>TARBP2</i>. These results provided a novel resource for elucidating regulatory mechanisms underlying STH sheep prolificacy. |
| first_indexed | 2024-03-09T16:47:57Z |
| format | Article |
| id | doaj.art-7ab30fa6e8144046ada33dde7d3b8d91 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T16:47:57Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-7ab30fa6e8144046ada33dde7d3b8d912023-11-24T14:43:47ZengMDPI AGGenes2073-44252023-10-011411202410.3390/genes14112024Thyroid Transcriptomics Revealed the Reproductive Regulation of miRNA in the Follicular and Luteal Phases in Small-Tail Han Sheep with Different FecB GenotypesCheng Chang0Xiaoyun He1Ran Di2Xiangyu Wang3Miaoceng Han4Chen Liang5Mingxing Chu6State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaCollege of Animal Science, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Jinzhong 030801, ChinaState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaMicroRNA (miRNA) is a type of endogenous short−stranded ncRNA that influences many biological processes such as animal growth, development and metabolism. The thyroid gland is an important endocrine gland in sheep, and an increasing number of studies have shown that the thyroid gland plays an important role in animal reproduction, but the molecular mechanisms of the thyroid gland in sheep reproduction are poorly understood. In this study, RNA-seq was used to detect transcriptome expression patterns in the thyroid gland between the follicular phase (FP) and luteal phase (LP) in <i>FecB BB</i> (MM) and <i>FecB</i> ++ (ww) small-tail Han (STH) sheep, respectively, and to identify differentially expressed miRNAs (DEMs) associated with reproduction. Bioinformatic analysis of the target genes of these DEMs revealed that they can be enriched in multiple GO terms associated with the reproductive process in animals and in the KEGG signaling pathway. The miRNA–mRNA coexpression network revealed that oar-miR-133 and oar-miR-370-3p may play an important role in sheep reproduction. The results of the dual-luciferase reporter assay suggest a possible targeting relationship between novel-51 and <i>TARBP2</i>. These results provided a novel resource for elucidating regulatory mechanisms underlying STH sheep prolificacy.https://www.mdpi.com/2073-4425/14/11/2024small-tail Han sheepfollicular phaseluteal phasethyroid glandlitter sizemiRNA |
| spellingShingle | Cheng Chang Xiaoyun He Ran Di Xiangyu Wang Miaoceng Han Chen Liang Mingxing Chu Thyroid Transcriptomics Revealed the Reproductive Regulation of miRNA in the Follicular and Luteal Phases in Small-Tail Han Sheep with Different FecB Genotypes Genes small-tail Han sheep follicular phase luteal phase thyroid gland litter size miRNA |
| title | Thyroid Transcriptomics Revealed the Reproductive Regulation of miRNA in the Follicular and Luteal Phases in Small-Tail Han Sheep with Different FecB Genotypes |
| title_full | Thyroid Transcriptomics Revealed the Reproductive Regulation of miRNA in the Follicular and Luteal Phases in Small-Tail Han Sheep with Different FecB Genotypes |
| title_fullStr | Thyroid Transcriptomics Revealed the Reproductive Regulation of miRNA in the Follicular and Luteal Phases in Small-Tail Han Sheep with Different FecB Genotypes |
| title_full_unstemmed | Thyroid Transcriptomics Revealed the Reproductive Regulation of miRNA in the Follicular and Luteal Phases in Small-Tail Han Sheep with Different FecB Genotypes |
| title_short | Thyroid Transcriptomics Revealed the Reproductive Regulation of miRNA in the Follicular and Luteal Phases in Small-Tail Han Sheep with Different FecB Genotypes |
| title_sort | thyroid transcriptomics revealed the reproductive regulation of mirna in the follicular and luteal phases in small tail han sheep with different fecb genotypes |
| topic | small-tail Han sheep follicular phase luteal phase thyroid gland litter size miRNA |
| url | https://www.mdpi.com/2073-4425/14/11/2024 |
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