First Report of <i>aac(6′)-Ib</i> and <i>aac(6′)-Ib-cr</i> Variant Genes Associated with Mutations in <i>gyrA</i> Encoded Fluoroquinolone Resistance in Avian <i>Campylobacter coli</i> Strains Collected in Tunisia
The <i>aac(6′)-Ib</i> gene is the most widespread gene encoding aminoglycoside-modifying enzyme and conferring resistance to tobramycin, streptomycin and kanamycin. The variant <i>aac(6′)-Ib-cr</i> gene confers resistance to both aminoglycosides and fluoroquinolones (FQ). A t...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/22/16116 |
| Summary: | The <i>aac(6′)-Ib</i> gene is the most widespread gene encoding aminoglycoside-modifying enzyme and conferring resistance to tobramycin, streptomycin and kanamycin. The variant <i>aac(6′)-Ib-cr</i> gene confers resistance to both aminoglycosides and fluoroquinolones (FQ). A total of 132 <i>Campylobacter</i> isolates, including 91 <i>C. jejuni</i> and 41 <i>C. coli</i>, were selected from broiler hens isolates. The <i>aac(6′)-Ib</i> gene was amplified using PCR and was subsequently digested with the <i>BtsCI</i> restriction enzyme to identify <i>aac(6′)-Ib-cr</i>. Among these isolates, 31 out of 41 <i>C. coli</i> (75.6%) and 1 (0.98%) <i>C. jejuni</i> were positive for the <i>aac(6′)</i>-Ib gene, which was identified as the <i>aac(6′)-Ib-cr</i> variant in 10 (32.25%) <i>C. coli</i> isolates. This variant was correlated with mutations in gyrA (Thr-86-Ile), as well as resistance to FQs. This study is the first report in Tunisia on <i>Campylobacter coli</i> strains harboring both the <i>aac(6′)-Ib</i> and <i>aac(6′)-Ib-cr</i> variants. These genes were present in <i>Campylobacter</i> isolates exhibiting resistance to multiple antibiotics, which restricts the range of available treatments. |
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| ISSN: | 1661-6596 1422-0067 |