First Report of <i>aac(6′)-Ib</i> and <i>aac(6′)-Ib-cr</i> Variant Genes Associated with Mutations in <i>gyrA</i> Encoded Fluoroquinolone Resistance in Avian <i>Campylobacter coli</i> Strains Collected in Tunisia
The <i>aac(6′)-Ib</i> gene is the most widespread gene encoding aminoglycoside-modifying enzyme and conferring resistance to tobramycin, streptomycin and kanamycin. The variant <i>aac(6′)-Ib-cr</i> gene confers resistance to both aminoglycosides and fluoroquinolones (FQ). A t...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/22/16116 |
| _version_ | 1797459059973029888 |
|---|---|
| author | Manel Gharbi Mohammed Abdo Saghir Abbas Safa Hamrouni Abderrazak Maaroufi |
| author_facet | Manel Gharbi Mohammed Abdo Saghir Abbas Safa Hamrouni Abderrazak Maaroufi |
| author_sort | Manel Gharbi |
| collection | DOAJ |
| description | The <i>aac(6′)-Ib</i> gene is the most widespread gene encoding aminoglycoside-modifying enzyme and conferring resistance to tobramycin, streptomycin and kanamycin. The variant <i>aac(6′)-Ib-cr</i> gene confers resistance to both aminoglycosides and fluoroquinolones (FQ). A total of 132 <i>Campylobacter</i> isolates, including 91 <i>C. jejuni</i> and 41 <i>C. coli</i>, were selected from broiler hens isolates. The <i>aac(6′)-Ib</i> gene was amplified using PCR and was subsequently digested with the <i>BtsCI</i> restriction enzyme to identify <i>aac(6′)-Ib-cr</i>. Among these isolates, 31 out of 41 <i>C. coli</i> (75.6%) and 1 (0.98%) <i>C. jejuni</i> were positive for the <i>aac(6′)</i>-Ib gene, which was identified as the <i>aac(6′)-Ib-cr</i> variant in 10 (32.25%) <i>C. coli</i> isolates. This variant was correlated with mutations in gyrA (Thr-86-Ile), as well as resistance to FQs. This study is the first report in Tunisia on <i>Campylobacter coli</i> strains harboring both the <i>aac(6′)-Ib</i> and <i>aac(6′)-Ib-cr</i> variants. These genes were present in <i>Campylobacter</i> isolates exhibiting resistance to multiple antibiotics, which restricts the range of available treatments. |
| first_indexed | 2024-03-09T16:45:59Z |
| format | Article |
| id | doaj.art-7ab67ea83fd14156bd9950fb2caa089e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:45:59Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7ab67ea83fd14156bd9950fb2caa089e2023-11-24T14:46:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124221611610.3390/ijms242216116First Report of <i>aac(6′)-Ib</i> and <i>aac(6′)-Ib-cr</i> Variant Genes Associated with Mutations in <i>gyrA</i> Encoded Fluoroquinolone Resistance in Avian <i>Campylobacter coli</i> Strains Collected in TunisiaManel Gharbi0Mohammed Abdo Saghir Abbas1Safa Hamrouni2Abderrazak Maaroufi3Group of Bacteriology and Biotechnology Development, Laboratory of Epidemiology and Veterinary Microbiology, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1002, TunisiaUnit of Vector Ecology, Pasteur Institute of Tunis, Tunis 1002, TunisiaGroup of Bacteriology and Biotechnology Development, Laboratory of Epidemiology and Veterinary Microbiology, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1002, TunisiaGroup of Bacteriology and Biotechnology Development, Laboratory of Epidemiology and Veterinary Microbiology, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1002, TunisiaThe <i>aac(6′)-Ib</i> gene is the most widespread gene encoding aminoglycoside-modifying enzyme and conferring resistance to tobramycin, streptomycin and kanamycin. The variant <i>aac(6′)-Ib-cr</i> gene confers resistance to both aminoglycosides and fluoroquinolones (FQ). A total of 132 <i>Campylobacter</i> isolates, including 91 <i>C. jejuni</i> and 41 <i>C. coli</i>, were selected from broiler hens isolates. The <i>aac(6′)-Ib</i> gene was amplified using PCR and was subsequently digested with the <i>BtsCI</i> restriction enzyme to identify <i>aac(6′)-Ib-cr</i>. Among these isolates, 31 out of 41 <i>C. coli</i> (75.6%) and 1 (0.98%) <i>C. jejuni</i> were positive for the <i>aac(6′)</i>-Ib gene, which was identified as the <i>aac(6′)-Ib-cr</i> variant in 10 (32.25%) <i>C. coli</i> isolates. This variant was correlated with mutations in gyrA (Thr-86-Ile), as well as resistance to FQs. This study is the first report in Tunisia on <i>Campylobacter coli</i> strains harboring both the <i>aac(6′)-Ib</i> and <i>aac(6′)-Ib-cr</i> variants. These genes were present in <i>Campylobacter</i> isolates exhibiting resistance to multiple antibiotics, which restricts the range of available treatments.https://www.mdpi.com/1422-0067/24/22/16116<i>Campylobacter</i>poultryfluoroquinolone-resistancephylogeny<i>aac(6′)-Ib-cr</i> |
| spellingShingle | Manel Gharbi Mohammed Abdo Saghir Abbas Safa Hamrouni Abderrazak Maaroufi First Report of <i>aac(6′)-Ib</i> and <i>aac(6′)-Ib-cr</i> Variant Genes Associated with Mutations in <i>gyrA</i> Encoded Fluoroquinolone Resistance in Avian <i>Campylobacter coli</i> Strains Collected in Tunisia International Journal of Molecular Sciences <i>Campylobacter</i> poultry fluoroquinolone-resistance phylogeny <i>aac(6′)-Ib-cr</i> |
| title | First Report of <i>aac(6′)-Ib</i> and <i>aac(6′)-Ib-cr</i> Variant Genes Associated with Mutations in <i>gyrA</i> Encoded Fluoroquinolone Resistance in Avian <i>Campylobacter coli</i> Strains Collected in Tunisia |
| title_full | First Report of <i>aac(6′)-Ib</i> and <i>aac(6′)-Ib-cr</i> Variant Genes Associated with Mutations in <i>gyrA</i> Encoded Fluoroquinolone Resistance in Avian <i>Campylobacter coli</i> Strains Collected in Tunisia |
| title_fullStr | First Report of <i>aac(6′)-Ib</i> and <i>aac(6′)-Ib-cr</i> Variant Genes Associated with Mutations in <i>gyrA</i> Encoded Fluoroquinolone Resistance in Avian <i>Campylobacter coli</i> Strains Collected in Tunisia |
| title_full_unstemmed | First Report of <i>aac(6′)-Ib</i> and <i>aac(6′)-Ib-cr</i> Variant Genes Associated with Mutations in <i>gyrA</i> Encoded Fluoroquinolone Resistance in Avian <i>Campylobacter coli</i> Strains Collected in Tunisia |
| title_short | First Report of <i>aac(6′)-Ib</i> and <i>aac(6′)-Ib-cr</i> Variant Genes Associated with Mutations in <i>gyrA</i> Encoded Fluoroquinolone Resistance in Avian <i>Campylobacter coli</i> Strains Collected in Tunisia |
| title_sort | first report of i aac 6 ib i and i aac 6 ib cr i variant genes associated with mutations in i gyra i encoded fluoroquinolone resistance in avian i campylobacter coli i strains collected in tunisia |
| topic | <i>Campylobacter</i> poultry fluoroquinolone-resistance phylogeny <i>aac(6′)-Ib-cr</i> |
| url | https://www.mdpi.com/1422-0067/24/22/16116 |
| work_keys_str_mv | AT manelgharbi firstreportofiaac6ibiandiaac6ibcrivariantgenesassociatedwithmutationsinigyraiencodedfluoroquinoloneresistanceinavianicampylobactercoliistrainscollectedintunisia AT mohammedabdosaghirabbas firstreportofiaac6ibiandiaac6ibcrivariantgenesassociatedwithmutationsinigyraiencodedfluoroquinoloneresistanceinavianicampylobactercoliistrainscollectedintunisia AT safahamrouni firstreportofiaac6ibiandiaac6ibcrivariantgenesassociatedwithmutationsinigyraiencodedfluoroquinoloneresistanceinavianicampylobactercoliistrainscollectedintunisia AT abderrazakmaaroufi firstreportofiaac6ibiandiaac6ibcrivariantgenesassociatedwithmutationsinigyraiencodedfluoroquinoloneresistanceinavianicampylobactercoliistrainscollectedintunisia |