Identification, Analysis and Gene Cloning of the SWEET Gene Family Provide Insights into Sugar Transport in Pomegranate (<i>Punica granatum</i>)
Members of the sugars will eventually be exported transporter (SWEET) family regulate the transport of different sugars through the cell membrane and control the distribution of sugars inside and outside the cell. The SWEET gene family also plays important roles in plant growth and development and p...
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MDPI AG
2022-02-01
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| Series: | International Journal of Molecular Sciences |
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| author | Xinhui Zhang Sha Wang Yuan Ren Chengyan Gan Bianbian Li Yaoyuwei Fan Xueqing Zhao Zhaohe Yuan |
| author_facet | Xinhui Zhang Sha Wang Yuan Ren Chengyan Gan Bianbian Li Yaoyuwei Fan Xueqing Zhao Zhaohe Yuan |
| author_sort | Xinhui Zhang |
| collection | DOAJ |
| description | Members of the sugars will eventually be exported transporter (SWEET) family regulate the transport of different sugars through the cell membrane and control the distribution of sugars inside and outside the cell. The SWEET gene family also plays important roles in plant growth and development and physiological processes. So far, there are no reports on the SWEET family in pomegranate. Meanwhile, pomegranate is rich in sugar, and three published pomegranate genome sequences provide resources for the study of the SWEET gene family. 20 PgSWEETs from pomegranate and the known <i>Arabidopsis</i> and grape SWEETs were divided into four clades (Ⅰ, Ⅱ, Ⅲ and Ⅳ) according to the phylogenetic relationships. PgSWEETs of the same clade share similar gene structures, predicting their similar biological functions. RNA-Seq data suggested that PgSWEET genes have a tissue-specific expression pattern. Foliar application of tripotassium phosphate significantly increased the total soluble sugar content of pomegranate fruits and leaves and significantly affected the expression levels of PgSWEETs. The plant growth hormone regulator assay also significantly affected the PgSWEETs expression both in buds of bisexual and functional male flowers. Among them, we selected <i>PgSWEET17a</i> as a candidate gene that plays a role in fructose transport in leaves. The 798 bp CDS sequence of <i>PgSWEET17a</i> was cloned, which encodes 265 amino acids. The subcellular localization of PgSWEET17a showed that it was localized to the cell membrane, indicating its involvement in sugar transport. Transient expression results showed that tobacco fructose content was significantly increased with the up-regulation of <i>PgSWEET17a</i>, while both sucrose and glucose contents were significantly down-regulated. The integration of the PgSWEET phylogenetic tree, gene structure and RNA-Seq data provide a genome-wide trait and expression pattern. Our findings suggest that tripotassium phosphate and plant exogenous hormone treatments could alter PgSWEET expression patterns. These provide a reference for further functional verification and sugar metabolism pathway regulation of PgSWEETs. |
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| language | English |
| last_indexed | 2024-03-09T20:38:58Z |
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| spelling | doaj.art-7ab763982a2943ea81f024c573e790e72023-11-23T23:04:33ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-02-01235247110.3390/ijms23052471Identification, Analysis and Gene Cloning of the SWEET Gene Family Provide Insights into Sugar Transport in Pomegranate (<i>Punica granatum</i>)Xinhui Zhang0Sha Wang1Yuan Ren2Chengyan Gan3Bianbian Li4Yaoyuwei Fan5Xueqing Zhao6Zhaohe Yuan7Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaMembers of the sugars will eventually be exported transporter (SWEET) family regulate the transport of different sugars through the cell membrane and control the distribution of sugars inside and outside the cell. The SWEET gene family also plays important roles in plant growth and development and physiological processes. So far, there are no reports on the SWEET family in pomegranate. Meanwhile, pomegranate is rich in sugar, and three published pomegranate genome sequences provide resources for the study of the SWEET gene family. 20 PgSWEETs from pomegranate and the known <i>Arabidopsis</i> and grape SWEETs were divided into four clades (Ⅰ, Ⅱ, Ⅲ and Ⅳ) according to the phylogenetic relationships. PgSWEETs of the same clade share similar gene structures, predicting their similar biological functions. RNA-Seq data suggested that PgSWEET genes have a tissue-specific expression pattern. Foliar application of tripotassium phosphate significantly increased the total soluble sugar content of pomegranate fruits and leaves and significantly affected the expression levels of PgSWEETs. The plant growth hormone regulator assay also significantly affected the PgSWEETs expression both in buds of bisexual and functional male flowers. Among them, we selected <i>PgSWEET17a</i> as a candidate gene that plays a role in fructose transport in leaves. The 798 bp CDS sequence of <i>PgSWEET17a</i> was cloned, which encodes 265 amino acids. The subcellular localization of PgSWEET17a showed that it was localized to the cell membrane, indicating its involvement in sugar transport. Transient expression results showed that tobacco fructose content was significantly increased with the up-regulation of <i>PgSWEET17a</i>, while both sucrose and glucose contents were significantly down-regulated. The integration of the PgSWEET phylogenetic tree, gene structure and RNA-Seq data provide a genome-wide trait and expression pattern. Our findings suggest that tripotassium phosphate and plant exogenous hormone treatments could alter PgSWEET expression patterns. These provide a reference for further functional verification and sugar metabolism pathway regulation of PgSWEETs.https://www.mdpi.com/1422-0067/23/5/2471pomegranateSWEET gene familygene expressiongene cloningsubcellular localization |
| spellingShingle | Xinhui Zhang Sha Wang Yuan Ren Chengyan Gan Bianbian Li Yaoyuwei Fan Xueqing Zhao Zhaohe Yuan Identification, Analysis and Gene Cloning of the SWEET Gene Family Provide Insights into Sugar Transport in Pomegranate (<i>Punica granatum</i>) International Journal of Molecular Sciences pomegranate SWEET gene family gene expression gene cloning subcellular localization |
| title | Identification, Analysis and Gene Cloning of the SWEET Gene Family Provide Insights into Sugar Transport in Pomegranate (<i>Punica granatum</i>) |
| title_full | Identification, Analysis and Gene Cloning of the SWEET Gene Family Provide Insights into Sugar Transport in Pomegranate (<i>Punica granatum</i>) |
| title_fullStr | Identification, Analysis and Gene Cloning of the SWEET Gene Family Provide Insights into Sugar Transport in Pomegranate (<i>Punica granatum</i>) |
| title_full_unstemmed | Identification, Analysis and Gene Cloning of the SWEET Gene Family Provide Insights into Sugar Transport in Pomegranate (<i>Punica granatum</i>) |
| title_short | Identification, Analysis and Gene Cloning of the SWEET Gene Family Provide Insights into Sugar Transport in Pomegranate (<i>Punica granatum</i>) |
| title_sort | identification analysis and gene cloning of the sweet gene family provide insights into sugar transport in pomegranate i punica granatum i |
| topic | pomegranate SWEET gene family gene expression gene cloning subcellular localization |
| url | https://www.mdpi.com/1422-0067/23/5/2471 |
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