A method for high‐throughput production of sequence‐verified DNA libraries and strain collections

A method for high‐throughput production of sequence‐verified DNA libraries and strain collections

Abstract The low costs of array‐synthesized oligonucleotide libraries are empowering rapid advances in quantitative and synthetic biology. However, high synthesis error rates, uneven representation, and lack of access to individual oligonucleotides limit the true potential of these libraries. We hav...

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Bibliographic Details
Main Authors: Justin D Smith, Ulrich Schlecht, Weihong Xu, Sundari Suresh, Joe Horecka, Michael J Proctor, Raeka S Aiyar, Richard A O Bennett, Angela Chu, Yong Fuga Li, Kevin Roy, Ronald W Davis, Lars M Steinmetz, Richard W Hyman, Sasha F Levy, Robert P St.Onge
Format: Article
Language:English
Published: Springer Nature 2017-02-01
Series:Molecular Systems Biology
Subjects:
arrayed strain collection
CRISPR interference
DNA libraries
oligonucleotide pools
synthetic biology
Online Access:https://doi.org/10.15252/msb.20167233

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