Single-cell transcriptome analysis for cancer and biology of the pancreas: A review on recent progress
Single-cell sequencing has become one of the most used techniques across the wide field of biology. It has enabled researchers to investigate the whole transcriptome at the cellular level across tissues, which unlocks numerous potentials for basic and applied studies in future diagnosis and therapy....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2023.1029758/full |
| _version_ | 1797851375135096832 |
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| author | Mona Tamaddon Mostafa Azimzadeh Mostafa Azimzadeh Mostafa Azimzadeh Peyman Gifani Peyman Gifani Seyed Mohammad Tavangar Seyed Mohammad Tavangar |
| author_facet | Mona Tamaddon Mostafa Azimzadeh Mostafa Azimzadeh Mostafa Azimzadeh Peyman Gifani Peyman Gifani Seyed Mohammad Tavangar Seyed Mohammad Tavangar |
| author_sort | Mona Tamaddon |
| collection | DOAJ |
| description | Single-cell sequencing has become one of the most used techniques across the wide field of biology. It has enabled researchers to investigate the whole transcriptome at the cellular level across tissues, which unlocks numerous potentials for basic and applied studies in future diagnosis and therapy. Here, we review the impact of single-cell RNA sequencing, as the prominent single-cell technique, in pancreatic biology and cancer. We discuss the most recent findings about pancreatic physiology and pathophysiology owing to this technological advancement in the past few years. Using single-cell RNA sequencing, researchers have been able to discover cellular heterogeneity across healthy cell types, as well as cancer tissues of the pancreas. We will discuss the new immunological targets and new molecular mechanisms of progression in the microenvironment of pancreatic cancer studied using single-cell RNA sequencing. The scope is not limited to cancer tissues, and we cover novel developmental, evolutionary, physiological, and heterogenic insights that have also been achieved recently for pancreatic tissues. We cover all biological insights derived from the single-cell RNA sequencing data, discuss the corresponding pros and cons, and finally, conclude how future research can move better by utilizing single-cell analysis for pancreatic biology. |
| first_indexed | 2024-04-09T19:16:53Z |
| format | Article |
| id | doaj.art-7aba38f1148848b2aaa3d612c87c9e98 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-09T19:16:53Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-7aba38f1148848b2aaa3d612c87c9e982023-04-06T04:56:01ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-04-011410.3389/fgene.2023.10297581029758Single-cell transcriptome analysis for cancer and biology of the pancreas: A review on recent progressMona Tamaddon0Mostafa Azimzadeh1Mostafa Azimzadeh2Mostafa Azimzadeh3Peyman Gifani4Peyman Gifani5Seyed Mohammad Tavangar6Seyed Mohammad Tavangar7Chronic Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Biotechnology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranMedical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, IranStem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, IranAI VIVO Ltd., Bioinnovation Centre, Cambridge, United KingdomGenetic Department, Institute of Systems Biology, University of Cambridge, Cambridge, United KingdomChronic Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, IranDepartment of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, IranSingle-cell sequencing has become one of the most used techniques across the wide field of biology. It has enabled researchers to investigate the whole transcriptome at the cellular level across tissues, which unlocks numerous potentials for basic and applied studies in future diagnosis and therapy. Here, we review the impact of single-cell RNA sequencing, as the prominent single-cell technique, in pancreatic biology and cancer. We discuss the most recent findings about pancreatic physiology and pathophysiology owing to this technological advancement in the past few years. Using single-cell RNA sequencing, researchers have been able to discover cellular heterogeneity across healthy cell types, as well as cancer tissues of the pancreas. We will discuss the new immunological targets and new molecular mechanisms of progression in the microenvironment of pancreatic cancer studied using single-cell RNA sequencing. The scope is not limited to cancer tissues, and we cover novel developmental, evolutionary, physiological, and heterogenic insights that have also been achieved recently for pancreatic tissues. We cover all biological insights derived from the single-cell RNA sequencing data, discuss the corresponding pros and cons, and finally, conclude how future research can move better by utilizing single-cell analysis for pancreatic biology.https://www.frontiersin.org/articles/10.3389/fgene.2023.1029758/fullsingle-cell sequencingtranscriptome analysispancreas biologypancreas cancergenetic heterogeneity |
| spellingShingle | Mona Tamaddon Mostafa Azimzadeh Mostafa Azimzadeh Mostafa Azimzadeh Peyman Gifani Peyman Gifani Seyed Mohammad Tavangar Seyed Mohammad Tavangar Single-cell transcriptome analysis for cancer and biology of the pancreas: A review on recent progress Frontiers in Genetics single-cell sequencing transcriptome analysis pancreas biology pancreas cancer genetic heterogeneity |
| title | Single-cell transcriptome analysis for cancer and biology of the pancreas: A review on recent progress |
| title_full | Single-cell transcriptome analysis for cancer and biology of the pancreas: A review on recent progress |
| title_fullStr | Single-cell transcriptome analysis for cancer and biology of the pancreas: A review on recent progress |
| title_full_unstemmed | Single-cell transcriptome analysis for cancer and biology of the pancreas: A review on recent progress |
| title_short | Single-cell transcriptome analysis for cancer and biology of the pancreas: A review on recent progress |
| title_sort | single cell transcriptome analysis for cancer and biology of the pancreas a review on recent progress |
| topic | single-cell sequencing transcriptome analysis pancreas biology pancreas cancer genetic heterogeneity |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2023.1029758/full |
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