Cardioprotective effects of pomegranate juice against ischemia and reperfusion in isolated rat heart
Background: The literature regarding the cardiovascular effects of pomegranate has been expanding during recent years. The present study aimed to investigate the cardioprotective effect of pomegranate juice following ischemia and reperfusion.Methods: The animals were anesthetized with thiopental sod...
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| Format: | Article |
| Language: | English |
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Kerman University of Medical Sciences
2021-09-01
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| Series: | Journal of Kerman University of Medical Sciences |
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| Online Access: | https://jkmu.kmu.ac.ir/article_91760_3cdeacfe46df27f4a65091ce1617deaa.pdf |
| _version_ | 1827911927840374784 |
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| author | Kaveh Rahimi Hamid Reza Kazerani |
| author_facet | Kaveh Rahimi Hamid Reza Kazerani |
| author_sort | Kaveh Rahimi |
| collection | DOAJ |
| description | Background: The literature regarding the cardiovascular effects of pomegranate has been expanding during recent years. The present study aimed to investigate the cardioprotective effect of pomegranate juice following ischemia and reperfusion.Methods: The animals were anesthetized with thiopental sodium (60 mg kg-1 BW) and their hearts were removed and perfused using Langendorff technique. In the first stage of the experiment, pomegranate juice was dissolved in Krebs solution. The first group was the normoxic control; the remaining 4 groups underwent 30 min global ischemia followed by 90 min reperfusion and received pomegranate juice at 0, 1, 2, and 4% in Krebs solution, respectively. The heart rate, coronary perfusion pressure, left ventricular developed pressure, rate pressure product (RPP), and dp/dtmax were monitored throughout the experiment. The infarct size was also measured via staining with triphenyl tetrazolium chloride. In the second stage, the rats received either placebo or pomegranate juice (4 ml kg-1 BW), daily, for 3 weeks. The hearts isolated from these animals were then studied as mentioned earlier, but no juice was added to the perfusion solution.Results: The hearts treated with the juice showed significantly higher percentages of RPP and increased dp/dtmax during reperfusion, compared to the control group. Moreover, these hearts showed smaller infarct sizes. In the second stage of the study, all studied hemodynamics were significantly higher in the test group both before and after ischemia. The infarct size was also smaller in the test group.Conclusion: The results of the present study suggest strong cardioprotective effects of pomegranate juice against myocardial ischemia and reperfusion. |
| first_indexed | 2024-03-13T02:09:11Z |
| format | Article |
| id | doaj.art-7abe6ac496d64b389910c6bb2b074469 |
| institution | Directory Open Access Journal |
| issn | 2008-2843 |
| language | English |
| last_indexed | 2024-03-13T02:09:11Z |
| publishDate | 2021-09-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Journal of Kerman University of Medical Sciences |
| spelling | doaj.art-7abe6ac496d64b389910c6bb2b0744692023-07-01T05:11:54ZengKerman University of Medical SciencesJournal of Kerman University of Medical Sciences2008-28432021-09-0128543744410.22062/jkmu.2021.9176091760Cardioprotective effects of pomegranate juice against ischemia and reperfusion in isolated rat heartKaveh Rahimi0Hamid Reza Kazerani1Department of Physiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Physiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, IranBackground: The literature regarding the cardiovascular effects of pomegranate has been expanding during recent years. The present study aimed to investigate the cardioprotective effect of pomegranate juice following ischemia and reperfusion.Methods: The animals were anesthetized with thiopental sodium (60 mg kg-1 BW) and their hearts were removed and perfused using Langendorff technique. In the first stage of the experiment, pomegranate juice was dissolved in Krebs solution. The first group was the normoxic control; the remaining 4 groups underwent 30 min global ischemia followed by 90 min reperfusion and received pomegranate juice at 0, 1, 2, and 4% in Krebs solution, respectively. The heart rate, coronary perfusion pressure, left ventricular developed pressure, rate pressure product (RPP), and dp/dtmax were monitored throughout the experiment. The infarct size was also measured via staining with triphenyl tetrazolium chloride. In the second stage, the rats received either placebo or pomegranate juice (4 ml kg-1 BW), daily, for 3 weeks. The hearts isolated from these animals were then studied as mentioned earlier, but no juice was added to the perfusion solution.Results: The hearts treated with the juice showed significantly higher percentages of RPP and increased dp/dtmax during reperfusion, compared to the control group. Moreover, these hearts showed smaller infarct sizes. In the second stage of the study, all studied hemodynamics were significantly higher in the test group both before and after ischemia. The infarct size was also smaller in the test group.Conclusion: The results of the present study suggest strong cardioprotective effects of pomegranate juice against myocardial ischemia and reperfusion.https://jkmu.kmu.ac.ir/article_91760_3cdeacfe46df27f4a65091ce1617deaa.pdfpomegranatepunica granatumischemic heartreperfusion |
| spellingShingle | Kaveh Rahimi Hamid Reza Kazerani Cardioprotective effects of pomegranate juice against ischemia and reperfusion in isolated rat heart Journal of Kerman University of Medical Sciences pomegranate punica granatum ischemic heart reperfusion |
| title | Cardioprotective effects of pomegranate juice against ischemia and reperfusion in isolated rat heart |
| title_full | Cardioprotective effects of pomegranate juice against ischemia and reperfusion in isolated rat heart |
| title_fullStr | Cardioprotective effects of pomegranate juice against ischemia and reperfusion in isolated rat heart |
| title_full_unstemmed | Cardioprotective effects of pomegranate juice against ischemia and reperfusion in isolated rat heart |
| title_short | Cardioprotective effects of pomegranate juice against ischemia and reperfusion in isolated rat heart |
| title_sort | cardioprotective effects of pomegranate juice against ischemia and reperfusion in isolated rat heart |
| topic | pomegranate punica granatum ischemic heart reperfusion |
| url | https://jkmu.kmu.ac.ir/article_91760_3cdeacfe46df27f4a65091ce1617deaa.pdf |
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