Studies on the compartmentation of lipid in adipose cells. II. Cholesterol accumulation and distribution in adipose tissue components
Adipose tissue was shown to contain 0.6–1.6 mg of cholesterol per gram wet weight. When expressed per unit of protein or organ mass, fat tissue contains more cholesterol than most other organs or membranes. The cholesterol content of fat tissue increased with the age and weight of the rat. Over 95%...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1973-05-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520368942 |
| _version_ | 1818716141441253376 |
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| author | J. Farkas A. Angel M.I. Avigan |
| author_facet | J. Farkas A. Angel M.I. Avigan |
| author_sort | J. Farkas |
| collection | DOAJ |
| description | Adipose tissue was shown to contain 0.6–1.6 mg of cholesterol per gram wet weight. When expressed per unit of protein or organ mass, fat tissue contains more cholesterol than most other organs or membranes. The cholesterol content of fat tissue increased with the age and weight of the rat. Over 95% of adipose tissue sterols was cholesterol, and most of it was free. In young (150–165 g) rats two-thirds of fat tissue cholesterol was in collagenase-derived adipocytes while in older rats (450–480 g) 90% of fat tissue cholesterol was in adipocytes and the remainder was in stromal–vascular elements. Age-related differences in subcellular cholesterol distribution were also observed. The cholesterol/phospholipid molar ratios of purified plasma membrane fractions from small and large fat cells were identical (0.22–0.25), thus resembling muscle and liver membranes. 7.5 days after intravenous administration of [4-14C]cholesterol the specific activity of adipose cholesterol exceeded that of plasma cholesterol. At 28 days the specific activity of adipose and muscle cholesterol exceeded that of plasma three- to fivefold. The t½ disappearance of adipose tissue cholesterol was approximately 27 days, which is consistent with its function as a slowly turning over storage pool.Thus, fat tissue is a major cholesterol storage organ. This may well account for the marked expansion of the slowly exchangeable cholesterol pool (pool B) observed in obesity. |
| first_indexed | 2024-12-17T19:14:32Z |
| format | Article |
| id | doaj.art-7ac0cbe1a48243a19315565f0ef4a2c2 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T19:14:32Z |
| publishDate | 1973-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-7ac0cbe1a48243a19315565f0ef4a2c22022-12-21T21:35:47ZengElsevierJournal of Lipid Research0022-22751973-05-01143344356Studies on the compartmentation of lipid in adipose cells. II. Cholesterol accumulation and distribution in adipose tissue componentsJ. Farkas0A. Angel1M.I. Avigan2Department of Medicine and Institute of Medical Science, University of Toronto, CanadaDepartment of Medicine and Institute of Medical Science, University of Toronto, CanadaDepartment of Medicine and Institute of Medical Science, University of Toronto, CanadaAdipose tissue was shown to contain 0.6–1.6 mg of cholesterol per gram wet weight. When expressed per unit of protein or organ mass, fat tissue contains more cholesterol than most other organs or membranes. The cholesterol content of fat tissue increased with the age and weight of the rat. Over 95% of adipose tissue sterols was cholesterol, and most of it was free. In young (150–165 g) rats two-thirds of fat tissue cholesterol was in collagenase-derived adipocytes while in older rats (450–480 g) 90% of fat tissue cholesterol was in adipocytes and the remainder was in stromal–vascular elements. Age-related differences in subcellular cholesterol distribution were also observed. The cholesterol/phospholipid molar ratios of purified plasma membrane fractions from small and large fat cells were identical (0.22–0.25), thus resembling muscle and liver membranes. 7.5 days after intravenous administration of [4-14C]cholesterol the specific activity of adipose cholesterol exceeded that of plasma cholesterol. At 28 days the specific activity of adipose and muscle cholesterol exceeded that of plasma three- to fivefold. The t½ disappearance of adipose tissue cholesterol was approximately 27 days, which is consistent with its function as a slowly turning over storage pool.Thus, fat tissue is a major cholesterol storage organ. This may well account for the marked expansion of the slowly exchangeable cholesterol pool (pool B) observed in obesity.http://www.sciencedirect.com/science/article/pii/S0022227520368942cholesterol metabolismcholesterol turnoverobesityplasma membranemembrane compositionmembrane cholesterol |
| spellingShingle | J. Farkas A. Angel M.I. Avigan Studies on the compartmentation of lipid in adipose cells. II. Cholesterol accumulation and distribution in adipose tissue components Journal of Lipid Research cholesterol metabolism cholesterol turnover obesity plasma membrane membrane composition membrane cholesterol |
| title | Studies on the compartmentation of lipid in adipose cells. II. Cholesterol accumulation and distribution in adipose tissue components |
| title_full | Studies on the compartmentation of lipid in adipose cells. II. Cholesterol accumulation and distribution in adipose tissue components |
| title_fullStr | Studies on the compartmentation of lipid in adipose cells. II. Cholesterol accumulation and distribution in adipose tissue components |
| title_full_unstemmed | Studies on the compartmentation of lipid in adipose cells. II. Cholesterol accumulation and distribution in adipose tissue components |
| title_short | Studies on the compartmentation of lipid in adipose cells. II. Cholesterol accumulation and distribution in adipose tissue components |
| title_sort | studies on the compartmentation of lipid in adipose cells ii cholesterol accumulation and distribution in adipose tissue components |
| topic | cholesterol metabolism cholesterol turnover obesity plasma membrane membrane composition membrane cholesterol |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520368942 |
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