Optimal Production of Protein Hydrolysates from Monkfish By-Products: Chemical Features and Associated Biological Activities
The aim of this work was the recovery of protein substrates from monkfish waste (heads and viscera) generated in the on-board processing of this species. Initially, the effect of pH, temperature, and protease concentration was studied on mixtures of a 1:1 ratio (<i>w/v</i>) of monkfish h...
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MDPI AG
2020-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/18/4068 |
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| author | José Antonio Vázquez Araceli Menduíña Margarita Nogueira Ana I. Durán Noelia Sanz Jesus Valcarcel |
| author_facet | José Antonio Vázquez Araceli Menduíña Margarita Nogueira Ana I. Durán Noelia Sanz Jesus Valcarcel |
| author_sort | José Antonio Vázquez |
| collection | DOAJ |
| description | The aim of this work was the recovery of protein substrates from monkfish waste (heads and viscera) generated in the on-board processing of this species. Initially, the effect of pH, temperature, and protease concentration was studied on mixtures of a 1:1 ratio (<i>w/v</i>) of monkfish heads/water. The optimal conditions of proteolytic digestion were established at 57.4 °C, pH 8.31, [Alcalase] = 0.05% (<i>v/w</i>) for 3 h of hydrolysis. Later on, a set of hydrolysis at 5L-pH-stat reactor were run under the aforementioned conditions, confirming the validity of the optimization studies for the head and viscera of monkfish. Regarding the chemical properties of the fish protein hydrolysates (FPH), the yield of digestion was higher than 90% in both cases and the degrees of hydrolysis and the soluble protein content were not especially large (<20% and <45 g/L, respectively). In vitro digestibility was higher than 90% and the percentage of essential amino acids ranged from 40 to 42%. Antioxidant activities were higher in viscera FPH, and antihypertensive ability was superior in head FPH. The values of number average molecular weights (Mn) of monkfish hydrolysates were 600 Da in the viscera and 947 Da in the head. The peptide size distribution, obtained by size-exclusion chromatography, indicated that the largest presence of peptides below 1000 Da and 200 Da was observed in the viscera FPH. |
| first_indexed | 2024-03-10T16:32:50Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T16:32:50Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-7ac31bac75644b039e51bc8c761e64552023-11-20T12:44:17ZengMDPI AGMolecules1420-30492020-09-012518406810.3390/molecules25184068Optimal Production of Protein Hydrolysates from Monkfish By-Products: Chemical Features and Associated Biological ActivitiesJosé Antonio Vázquez0Araceli Menduíña1Margarita Nogueira2Ana I. Durán3Noelia Sanz4Jesus Valcarcel5Grupo de Biotecnología y Bioprocesos Marinos, Instituto de Investigaciones Marinas (IIM-CSIC), C/Eduardo Cabello, 6, CP 36208 Vigo, Galicia, SpainGrupo de Biotecnología y Bioprocesos Marinos, Instituto de Investigaciones Marinas (IIM-CSIC), C/Eduardo Cabello, 6, CP 36208 Vigo, Galicia, SpainGrupo de Biotecnología y Bioprocesos Marinos, Instituto de Investigaciones Marinas (IIM-CSIC), C/Eduardo Cabello, 6, CP 36208 Vigo, Galicia, SpainGrupo de Biotecnología y Bioprocesos Marinos, Instituto de Investigaciones Marinas (IIM-CSIC), C/Eduardo Cabello, 6, CP 36208 Vigo, Galicia, SpainGrupo de Biotecnología y Bioprocesos Marinos, Instituto de Investigaciones Marinas (IIM-CSIC), C/Eduardo Cabello, 6, CP 36208 Vigo, Galicia, SpainGrupo de Biotecnología y Bioprocesos Marinos, Instituto de Investigaciones Marinas (IIM-CSIC), C/Eduardo Cabello, 6, CP 36208 Vigo, Galicia, SpainThe aim of this work was the recovery of protein substrates from monkfish waste (heads and viscera) generated in the on-board processing of this species. Initially, the effect of pH, temperature, and protease concentration was studied on mixtures of a 1:1 ratio (<i>w/v</i>) of monkfish heads/water. The optimal conditions of proteolytic digestion were established at 57.4 °C, pH 8.31, [Alcalase] = 0.05% (<i>v/w</i>) for 3 h of hydrolysis. Later on, a set of hydrolysis at 5L-pH-stat reactor were run under the aforementioned conditions, confirming the validity of the optimization studies for the head and viscera of monkfish. Regarding the chemical properties of the fish protein hydrolysates (FPH), the yield of digestion was higher than 90% in both cases and the degrees of hydrolysis and the soluble protein content were not especially large (<20% and <45 g/L, respectively). In vitro digestibility was higher than 90% and the percentage of essential amino acids ranged from 40 to 42%. Antioxidant activities were higher in viscera FPH, and antihypertensive ability was superior in head FPH. The values of number average molecular weights (Mn) of monkfish hydrolysates were 600 Da in the viscera and 947 Da in the head. The peptide size distribution, obtained by size-exclusion chromatography, indicated that the largest presence of peptides below 1000 Da and 200 Da was observed in the viscera FPH.https://www.mdpi.com/1420-3049/25/18/4068protein recoverymonkfish by-productsfishing waste valorizationfish protein hydrolysatesbioactivesustainability |
| spellingShingle | José Antonio Vázquez Araceli Menduíña Margarita Nogueira Ana I. Durán Noelia Sanz Jesus Valcarcel Optimal Production of Protein Hydrolysates from Monkfish By-Products: Chemical Features and Associated Biological Activities Molecules protein recovery monkfish by-products fishing waste valorization fish protein hydrolysates bioactive sustainability |
| title | Optimal Production of Protein Hydrolysates from Monkfish By-Products: Chemical Features and Associated Biological Activities |
| title_full | Optimal Production of Protein Hydrolysates from Monkfish By-Products: Chemical Features and Associated Biological Activities |
| title_fullStr | Optimal Production of Protein Hydrolysates from Monkfish By-Products: Chemical Features and Associated Biological Activities |
| title_full_unstemmed | Optimal Production of Protein Hydrolysates from Monkfish By-Products: Chemical Features and Associated Biological Activities |
| title_short | Optimal Production of Protein Hydrolysates from Monkfish By-Products: Chemical Features and Associated Biological Activities |
| title_sort | optimal production of protein hydrolysates from monkfish by products chemical features and associated biological activities |
| topic | protein recovery monkfish by-products fishing waste valorization fish protein hydrolysates bioactive sustainability |
| url | https://www.mdpi.com/1420-3049/25/18/4068 |
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