The Mitochondrial Protein Translocation Motor: Structural Conservation between the Human and Yeast Tim14/Pam18-Tim16/Pam16 co-Chaperones
Most of our knowledge regarding the process of protein import into mitochondria has come from research employing Saccharomyces cerevisiae as a model system. Recently, several mammalian homologues of the mitochondrial motor proteins were identified. Of particular interest for us is the human Tim14/Pa...
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MDPI AG
2009-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/10/5/2041/ |
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| author | Shira Elsner Dana Simian Ohad Iosefson Milit Marom Abdussalam Azem |
| author_facet | Shira Elsner Dana Simian Ohad Iosefson Milit Marom Abdussalam Azem |
| author_sort | Shira Elsner |
| collection | DOAJ |
| description | Most of our knowledge regarding the process of protein import into mitochondria has come from research employing Saccharomyces cerevisiae as a model system. Recently, several mammalian homologues of the mitochondrial motor proteins were identified. Of particular interest for us is the human Tim14/Pam18-Tim16/Pam16 complex. We chose a structural approach in order to examine the evolutionary conservation between yeast Tim14/Pam18-Tim16/Pam16 proteins and their human homologues. For this purpose, we examined the structural properties of the purified human proteins and their interaction with their yeast homologues, in vitro. Our results show that the soluble domains of the human Tim14/Pam18 and Tim16/Pam16 proteins interact with their yeast counterparts, forming heterodimeric complexes and that these complexes interact with yeast mtHsp70. |
| first_indexed | 2024-04-13T02:56:00Z |
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| id | doaj.art-7ac56ac1733d48069dcfa657db5ab8c3 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T02:56:00Z |
| publishDate | 2009-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7ac56ac1733d48069dcfa657db5ab8c32022-12-22T03:05:39ZengMDPI AGInternational Journal of Molecular Sciences1422-00672009-05-011052041205310.3390/ijms10052041The Mitochondrial Protein Translocation Motor: Structural Conservation between the Human and Yeast Tim14/Pam18-Tim16/Pam16 co-ChaperonesShira ElsnerDana SimianOhad IosefsonMilit MaromAbdussalam AzemMost of our knowledge regarding the process of protein import into mitochondria has come from research employing Saccharomyces cerevisiae as a model system. Recently, several mammalian homologues of the mitochondrial motor proteins were identified. Of particular interest for us is the human Tim14/Pam18-Tim16/Pam16 complex. We chose a structural approach in order to examine the evolutionary conservation between yeast Tim14/Pam18-Tim16/Pam16 proteins and their human homologues. For this purpose, we examined the structural properties of the purified human proteins and their interaction with their yeast homologues, in vitro. Our results show that the soluble domains of the human Tim14/Pam18 and Tim16/Pam16 proteins interact with their yeast counterparts, forming heterodimeric complexes and that these complexes interact with yeast mtHsp70.http://www.mdpi.com/1422-0067/10/5/2041/Tim14 (also known as Pam18)Tim16 (also known as Pam16)mtHsp70translocation motor |
| spellingShingle | Shira Elsner Dana Simian Ohad Iosefson Milit Marom Abdussalam Azem The Mitochondrial Protein Translocation Motor: Structural Conservation between the Human and Yeast Tim14/Pam18-Tim16/Pam16 co-Chaperones International Journal of Molecular Sciences Tim14 (also known as Pam18) Tim16 (also known as Pam16) mtHsp70 translocation motor |
| title | The Mitochondrial Protein Translocation Motor: Structural Conservation between the Human and Yeast Tim14/Pam18-Tim16/Pam16 co-Chaperones |
| title_full | The Mitochondrial Protein Translocation Motor: Structural Conservation between the Human and Yeast Tim14/Pam18-Tim16/Pam16 co-Chaperones |
| title_fullStr | The Mitochondrial Protein Translocation Motor: Structural Conservation between the Human and Yeast Tim14/Pam18-Tim16/Pam16 co-Chaperones |
| title_full_unstemmed | The Mitochondrial Protein Translocation Motor: Structural Conservation between the Human and Yeast Tim14/Pam18-Tim16/Pam16 co-Chaperones |
| title_short | The Mitochondrial Protein Translocation Motor: Structural Conservation between the Human and Yeast Tim14/Pam18-Tim16/Pam16 co-Chaperones |
| title_sort | mitochondrial protein translocation motor structural conservation between the human and yeast tim14 pam18 tim16 pam16 co chaperones |
| topic | Tim14 (also known as Pam18) Tim16 (also known as Pam16) mtHsp70 translocation motor |
| url | http://www.mdpi.com/1422-0067/10/5/2041/ |
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