Effect of Bicomponent ZnO-ZnFe2O4 Nanoparticles on Mediterranean Mussel (Mytilus galloprovincialis) Hemocytes under in vitro Conditions
The present work investigates the toxic effect of bicomponent ZnO-ZnFe2O4 nanoparticles, which are the main active component of the domestic antifouling coating, on marker indicators of Mediterranean mussel (Mytilus galloprovincialis) hemolymph cells (hemocytes) under in vitro experimental condition...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Federal State Budget Scientific Institution «Marine Hydrophysical Institute of RAS»
2023-03-01
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| Series: | Экологическая безопасность прибрежной и шельфовой зон моря |
| Subjects: | |
| Online Access: | http://ecological-safety.ru/en/repository/issues/2023/01/10/20230110.pdf |
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| author | M.S. Podolskaya A.A. Tkachuk A.Yu. Andreyeva E.S. Kladchenko E.S. Chelebieva A.A. Mosunov |
| author_facet | M.S. Podolskaya A.A. Tkachuk A.Yu. Andreyeva E.S. Kladchenko E.S. Chelebieva A.A. Mosunov |
| author_sort | M.S. Podolskaya |
| collection | DOAJ |
| description | The present work investigates the toxic effect of bicomponent ZnO-ZnFe2O4 nanoparticles, which are the main active component of the domestic antifouling coating, on marker indicators of Mediterranean mussel (Mytilus galloprovincialis) hemolymph cells (hemocytes) under in vitro experimental conditions. The following indicators were evaluated: mortality, cellular composition and production of reactive oxygen species. In the experiment, hemocytes were incubated for 1 and 2 hours in 1 mL of sterile seawater containing nanoparticles of different concentrations: 0.03, 0.3 and 3 mg/mL. The data were analyzed using the flowing cytometry. It was shown that ZnО-ZnFe2O4 nanoparticles had an effect on the cellular composition of the hemolymph: the proportion of agranulocytes decreased and hour exposure to 0.03 mg/mL nanoparticles reduced the level of production of reactive oxygen species by 2.5 times compared to the control (p ≤ 0.05). Incubation of hemocytes with a maximum concentration of nanoparticles (3 mg/mL) led to cell death within 1 hour after exposure. No acute toxic effects on hemocytes with the use of 0.03 mg/mL and 0.3 mg/mL of zinc oxide and zinc ferrite nanoparticles were observed. |
| first_indexed | 2024-03-12T15:26:44Z |
| format | Article |
| id | doaj.art-7ac611eed108459d9b56d2722b37b894 |
| institution | Directory Open Access Journal |
| issn | 2413-5577 |
| language | English |
| last_indexed | 2024-03-12T15:26:44Z |
| publishDate | 2023-03-01 |
| publisher | Federal State Budget Scientific Institution «Marine Hydrophysical Institute of RAS» |
| record_format | Article |
| series | Экологическая безопасность прибрежной и шельфовой зон моря |
| spelling | doaj.art-7ac611eed108459d9b56d2722b37b8942023-08-10T13:07:36ZengFederal State Budget Scientific Institution «Marine Hydrophysical Institute of RAS»Экологическая безопасность прибрежной и шельфовой зон моря2413-55772023-03-011124136Effect of Bicomponent ZnO-ZnFe2O4 Nanoparticles on Mediterranean Mussel (Mytilus galloprovincialis) Hemocytes under in vitro ConditionsM.S. Podolskaya0A.A. Tkachuk1A.Yu. Andreyeva2E.S. Kladchenko3E.S. Chelebieva4A.A. Mosunov5A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russian FederationA.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russian FederationA.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russian FederationA.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russian FederationA.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russian FederationSevastopol State University, Sevastopol, Russian FederationThe present work investigates the toxic effect of bicomponent ZnO-ZnFe2O4 nanoparticles, which are the main active component of the domestic antifouling coating, on marker indicators of Mediterranean mussel (Mytilus galloprovincialis) hemolymph cells (hemocytes) under in vitro experimental conditions. The following indicators were evaluated: mortality, cellular composition and production of reactive oxygen species. In the experiment, hemocytes were incubated for 1 and 2 hours in 1 mL of sterile seawater containing nanoparticles of different concentrations: 0.03, 0.3 and 3 mg/mL. The data were analyzed using the flowing cytometry. It was shown that ZnО-ZnFe2O4 nanoparticles had an effect on the cellular composition of the hemolymph: the proportion of agranulocytes decreased and hour exposure to 0.03 mg/mL nanoparticles reduced the level of production of reactive oxygen species by 2.5 times compared to the control (p ≤ 0.05). Incubation of hemocytes with a maximum concentration of nanoparticles (3 mg/mL) led to cell death within 1 hour after exposure. No acute toxic effects on hemocytes with the use of 0.03 mg/mL and 0.3 mg/mL of zinc oxide and zinc ferrite nanoparticles were observed.http://ecological-safety.ru/en/repository/issues/2023/01/10/20230110.pdfnanoparticlesmediterranean musselhemocytesreactive oxygen species |
| spellingShingle | M.S. Podolskaya A.A. Tkachuk A.Yu. Andreyeva E.S. Kladchenko E.S. Chelebieva A.A. Mosunov Effect of Bicomponent ZnO-ZnFe2O4 Nanoparticles on Mediterranean Mussel (Mytilus galloprovincialis) Hemocytes under in vitro Conditions Экологическая безопасность прибрежной и шельфовой зон моря nanoparticles mediterranean mussel hemocytes reactive oxygen species |
| title | Effect of Bicomponent ZnO-ZnFe2O4 Nanoparticles on Mediterranean Mussel (Mytilus galloprovincialis) Hemocytes under in vitro Conditions |
| title_full | Effect of Bicomponent ZnO-ZnFe2O4 Nanoparticles on Mediterranean Mussel (Mytilus galloprovincialis) Hemocytes under in vitro Conditions |
| title_fullStr | Effect of Bicomponent ZnO-ZnFe2O4 Nanoparticles on Mediterranean Mussel (Mytilus galloprovincialis) Hemocytes under in vitro Conditions |
| title_full_unstemmed | Effect of Bicomponent ZnO-ZnFe2O4 Nanoparticles on Mediterranean Mussel (Mytilus galloprovincialis) Hemocytes under in vitro Conditions |
| title_short | Effect of Bicomponent ZnO-ZnFe2O4 Nanoparticles on Mediterranean Mussel (Mytilus galloprovincialis) Hemocytes under in vitro Conditions |
| title_sort | effect of bicomponent zno znfe2o4 nanoparticles on mediterranean mussel mytilus galloprovincialis hemocytes under in vitro conditions |
| topic | nanoparticles mediterranean mussel hemocytes reactive oxygen species |
| url | http://ecological-safety.ru/en/repository/issues/2023/01/10/20230110.pdf |
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