In Vitro Derivation of Quiescent Mouse Embryonic Stem Cells Based on Distinct Mitochondrial Activity
Summary: Embryonic diapause is a naturally occurring strategy in mammals that determines successful rates of gestation under unfavorable conditions. This dormant state can be captured in the form of quiescent mouse embryonic stem cells (ESCs). Here, we present a step-by-step protocol to derive quies...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | STAR Protocols |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720301234 |
| _version_ | 1818384506944487424 |
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| author | Le Tran Phuc Khoa Yali Dou |
| author_facet | Le Tran Phuc Khoa Yali Dou |
| author_sort | Le Tran Phuc Khoa |
| collection | DOAJ |
| description | Summary: Embryonic diapause is a naturally occurring strategy in mammals that determines successful rates of gestation under unfavorable conditions. This dormant state can be captured in the form of quiescent mouse embryonic stem cells (ESCs). Here, we present a step-by-step protocol to derive quiescent ESCs that naturally exist in culture by harnessing the heterogeneity of mitochondrial activity. The derived quiescent ESCs with low mitochondrial activity can be utilized as a surrogate to study stages of early embryonic development.For complete details on the use and execution of this protocol, please refer to Khoa et al. (2020). |
| first_indexed | 2024-12-14T03:23:21Z |
| format | Article |
| id | doaj.art-7ace20106cc14ffcbed4d54b01067488 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-14T03:23:21Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-7ace20106cc14ffcbed4d54b010674882022-12-21T23:18:58ZengElsevierSTAR Protocols2666-16672020-12-0113100136In Vitro Derivation of Quiescent Mouse Embryonic Stem Cells Based on Distinct Mitochondrial ActivityLe Tran Phuc Khoa0Yali Dou1Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Corresponding authorDepartment of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Corresponding authorSummary: Embryonic diapause is a naturally occurring strategy in mammals that determines successful rates of gestation under unfavorable conditions. This dormant state can be captured in the form of quiescent mouse embryonic stem cells (ESCs). Here, we present a step-by-step protocol to derive quiescent ESCs that naturally exist in culture by harnessing the heterogeneity of mitochondrial activity. The derived quiescent ESCs with low mitochondrial activity can be utilized as a surrogate to study stages of early embryonic development.For complete details on the use and execution of this protocol, please refer to Khoa et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720301234 |
| spellingShingle | Le Tran Phuc Khoa Yali Dou In Vitro Derivation of Quiescent Mouse Embryonic Stem Cells Based on Distinct Mitochondrial Activity STAR Protocols |
| title | In Vitro Derivation of Quiescent Mouse Embryonic Stem Cells Based on Distinct Mitochondrial Activity |
| title_full | In Vitro Derivation of Quiescent Mouse Embryonic Stem Cells Based on Distinct Mitochondrial Activity |
| title_fullStr | In Vitro Derivation of Quiescent Mouse Embryonic Stem Cells Based on Distinct Mitochondrial Activity |
| title_full_unstemmed | In Vitro Derivation of Quiescent Mouse Embryonic Stem Cells Based on Distinct Mitochondrial Activity |
| title_short | In Vitro Derivation of Quiescent Mouse Embryonic Stem Cells Based on Distinct Mitochondrial Activity |
| title_sort | in vitro derivation of quiescent mouse embryonic stem cells based on distinct mitochondrial activity |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720301234 |
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