Synthesis of silver nanoparticles using a Mentha spicata extract and evaluation of its anticancer and cytotoxic activity
In this study, silver nanoparticles (NP) were synthesized by two methods: using an aqueous extract of Mentha spicata leaves and using citrate ions as stabilizing agent, and the cytotoxicity and anticancer activity of both NP were evaluated in vitro. The particles synthesized with the aqueous extract...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2019-12-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/8142.pdf |
| _version_ | 1797419168219267072 |
|---|---|
| author | Yuridia Torres-Martínez Eder Arredondo-Espinoza Carlos Puente Omar González-Santiago Nayely Pineda-Aguilar Isaías Balderas-Rentería Israel López Mónica A. Ramírez-Cabrera |
| author_facet | Yuridia Torres-Martínez Eder Arredondo-Espinoza Carlos Puente Omar González-Santiago Nayely Pineda-Aguilar Isaías Balderas-Rentería Israel López Mónica A. Ramírez-Cabrera |
| author_sort | Yuridia Torres-Martínez |
| collection | DOAJ |
| description | In this study, silver nanoparticles (NP) were synthesized by two methods: using an aqueous extract of Mentha spicata leaves and using citrate ions as stabilizing agent, and the cytotoxicity and anticancer activity of both NP were evaluated in vitro. The particles synthesized with the aqueous extract were spherical with a size ranging from 15 to 45 nm. These NP decreased cell viability in all of the cells studied; however, the IC50 could only be estimated in the Chang liver cells (IC50 = 21.37 µg/mL). These particles also decreased the generation of reactive oxygen species in Chang and SiHa cells. Additionally, the dispersions decreased the activity of caspase-3. There was no significant difference between the biological activities of the NP obtained with the aqueous extract and the NP synthesized using citrate ions. This study showed that an aqueous extract of M. spicata is an excellent alternative for the synthesis of silver NP. These NP showed cytotoxicity and anticancer activity in vitro. Although more experiments are required, the cell death occurs probably through a mechanism different from apoptosis. |
| first_indexed | 2024-03-09T06:44:23Z |
| format | Article |
| id | doaj.art-7acf3d1a2d7d44b096b293e84c5c05c4 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:44:23Z |
| publishDate | 2019-12-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-7acf3d1a2d7d44b096b293e84c5c05c42023-12-03T10:40:51ZengPeerJ Inc.PeerJ2167-83592019-12-017e814210.7717/peerj.8142Synthesis of silver nanoparticles using a Mentha spicata extract and evaluation of its anticancer and cytotoxic activityYuridia Torres-Martínez0Eder Arredondo-Espinoza1Carlos Puente2Omar González-Santiago3Nayely Pineda-Aguilar4Isaías Balderas-Rentería5Israel López6Mónica A. Ramírez-Cabrera7Universidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Químicas, Laboratorio de Farmacología Molecular y Modelos Biológicos, Monterrey, Nuevo León, MexicoUniversidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Químicas, Laboratorio de Farmacología Molecular y Modelos Biológicos, Monterrey, Nuevo León, MexicoUniversidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Químicas, Laboratorio de Materiales I, San Nicolás de los Garza, Nuevo León, MexicoUniversidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Químicas, Laboratorio de Farmacología Molecular y Modelos Biológicos, Monterrey, Nuevo León, MexicoCentro de Investigación en Materiales Avanzados, S.C. (CIMAV), Unidad Monterrey, Apodaca, Nuevo León, MexicoUniversidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Químicas, Laboratorio de Farmacología Molecular y Modelos Biológicos, Monterrey, Nuevo León, MexicoUniversidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Químicas, Laboratorio de Materiales I, San Nicolás de los Garza, Nuevo León, MexicoUniversidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Químicas, Laboratorio de Farmacología Molecular y Modelos Biológicos, Monterrey, Nuevo León, MexicoIn this study, silver nanoparticles (NP) were synthesized by two methods: using an aqueous extract of Mentha spicata leaves and using citrate ions as stabilizing agent, and the cytotoxicity and anticancer activity of both NP were evaluated in vitro. The particles synthesized with the aqueous extract were spherical with a size ranging from 15 to 45 nm. These NP decreased cell viability in all of the cells studied; however, the IC50 could only be estimated in the Chang liver cells (IC50 = 21.37 µg/mL). These particles also decreased the generation of reactive oxygen species in Chang and SiHa cells. Additionally, the dispersions decreased the activity of caspase-3. There was no significant difference between the biological activities of the NP obtained with the aqueous extract and the NP synthesized using citrate ions. This study showed that an aqueous extract of M. spicata is an excellent alternative for the synthesis of silver NP. These NP showed cytotoxicity and anticancer activity in vitro. Although more experiments are required, the cell death occurs probably through a mechanism different from apoptosis.https://peerj.com/articles/8142.pdfSilver nanoparticlesAnticancerCitotoxicityMentha spicata |
| spellingShingle | Yuridia Torres-Martínez Eder Arredondo-Espinoza Carlos Puente Omar González-Santiago Nayely Pineda-Aguilar Isaías Balderas-Rentería Israel López Mónica A. Ramírez-Cabrera Synthesis of silver nanoparticles using a Mentha spicata extract and evaluation of its anticancer and cytotoxic activity PeerJ Silver nanoparticles Anticancer Citotoxicity Mentha spicata |
| title | Synthesis of silver nanoparticles using a Mentha spicata extract and evaluation of its anticancer and cytotoxic activity |
| title_full | Synthesis of silver nanoparticles using a Mentha spicata extract and evaluation of its anticancer and cytotoxic activity |
| title_fullStr | Synthesis of silver nanoparticles using a Mentha spicata extract and evaluation of its anticancer and cytotoxic activity |
| title_full_unstemmed | Synthesis of silver nanoparticles using a Mentha spicata extract and evaluation of its anticancer and cytotoxic activity |
| title_short | Synthesis of silver nanoparticles using a Mentha spicata extract and evaluation of its anticancer and cytotoxic activity |
| title_sort | synthesis of silver nanoparticles using a mentha spicata extract and evaluation of its anticancer and cytotoxic activity |
| topic | Silver nanoparticles Anticancer Citotoxicity Mentha spicata |
| url | https://peerj.com/articles/8142.pdf |
| work_keys_str_mv | AT yuridiatorresmartinez synthesisofsilvernanoparticlesusingamenthaspicataextractandevaluationofitsanticancerandcytotoxicactivity AT ederarredondoespinoza synthesisofsilvernanoparticlesusingamenthaspicataextractandevaluationofitsanticancerandcytotoxicactivity AT carlospuente synthesisofsilvernanoparticlesusingamenthaspicataextractandevaluationofitsanticancerandcytotoxicactivity AT omargonzalezsantiago synthesisofsilvernanoparticlesusingamenthaspicataextractandevaluationofitsanticancerandcytotoxicactivity AT nayelypinedaaguilar synthesisofsilvernanoparticlesusingamenthaspicataextractandevaluationofitsanticancerandcytotoxicactivity AT isaiasbalderasrenteria synthesisofsilvernanoparticlesusingamenthaspicataextractandevaluationofitsanticancerandcytotoxicactivity AT israellopez synthesisofsilvernanoparticlesusingamenthaspicataextractandevaluationofitsanticancerandcytotoxicactivity AT monicaaramirezcabrera synthesisofsilvernanoparticlesusingamenthaspicataextractandevaluationofitsanticancerandcytotoxicactivity |